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Çѱ¹È¯°æ»ý¹°ÇÐȸ / v.17, no.2, 1999³â, pp.209-215
¿Âµµ, pH ¹× ¿°µµ°¡ ±¼(Crassostrea gigas)ÀÇ MDH isozyme¿¡ ¹ÌÄ¡´Â ¿µÇâ
( Changes of the Malate Dehydrogenase Isozymes in Oyster (Crassostrea gigas) Exposed to Different Temperature, pH and Salinity )
±èÁö½Ä;±èÁ¾È¯; ±º»ê´ëÇб³ ÀÚ¿¬°úÇдëÇÐ »ý¹°Çаú;±º»ê´ëÇб³ ÀÚ¿¬°úÇдëÇÐ »ý¹°Çаú;
 
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¿Âµµ, pH¹× ¿°µµ stress°¡ ±¼(Crassostrea gigas)ÀÇ malate dehydrogenase isozyme¿¡ ¹ÌÄ¡´Â ¿µÇâÀ» Àü±â¿µµ¿¹ýÀ¸·Î ºÐ¼®ÇÏ¿´´Ù. ´ëÁ¶±ºÀÇ MDH isozymeÀº 2°³ÀÇ band°¡ ¾ç±Ø ÂÊÀ¸·Î À̵¿µÇ¾î ºÐ¸®µÇ¾ú´Ù. °¢ ½ÇÇè ¿Âµµ¿Í pH¸¦ 12½Ã°£ ³ëÃâ½ÃŲ ½ÇÇ豺¿¡¼­´Â 1°³ÀÇ band¸¸ ¾ç±Ø ÂÊ¿¡¼­ ºÐ¸®µÇ¾ú°í, 24½Ã°£°ú 48½Ã°£ ³ëÃâÇÑ ½ÇÇ豺¿¡¼­´Â 2°³ÀÇ band°¡ ¸ðµÎ ¾ç±Ø ÂÊ¿¡¼­ ºÐ¸®µÇ¾ú´Ù. ¿°ºÐ ³óµµ°¡ 5ppt ¿Í 3ppt¿¡¼­ 12½Ã°£ ³ëÃâ½ÃÄ×À» ¶§ °¢°¢ 2°³ÀÇ band°¡, 10ppt¿Í 40ppt¿¡¼­´Â °¢°¢ 3°³ÀÇ band°¡ ºÐ¸®µÇ¾ú°í, 24½Ã°£°ú 48½Ã°£ ³ëÃâ ½ÇÇ豺¿¡¼­´Â 2°³ÀÇ band°¡ ¾ç±Ø ÂÊ¿¡¼­ ºÐ¸®µÇ¾ú´Ù. ¸ðµç isozyme pattern¿¡¼­ °¢ bandÀÇ È°¼ºµµ´Â ½ÇÇè Á¶°Ç¿¡ µû¶ó °¢°¢ÀÇ Æ¯À̼ºÀÌ ³ªÅ¸³µ´Ù. °á°úÀûÀ¸·Î MDH isozymeÀÇ º¯È­´Â ±¼ÀÌ È¯°æ stress¿¡ ´ëÇÑ »ýÈ­ÇÐÀû ¹ÝÀÀÀÇ °á°ú·Î °£ÁֵȴÙ.
Changes of malate dehydrogenase isozyme in oyster exposed to different temperature, pH and salinity were investigated by polyacrylamide gel electrophoresis. MDH isozyme in control group was separated into two bands on the positive side. In case of temperature and pH stress, MDH isozyme was separated into only one band after 12 hours exposure but two bands after 24, 48 hours exposure on the positive side. In case of salinity stress, after 12 hours exposure, MDH isozyme bands were separated into two bands in 5 ppt, 30 ppt and three bands in 10 ppt, 40 ppt concentration on the positive side. After 24 hours and 48 hours exposure case in salinity stress, MDH isozyme bands was separated into two bands on the positive side in all concentration. Activities of isozyme bands show their characteristics according to the condition of experiment. In conclusion, changes of MDH isozyme was a biochemical defense mechanism in oyster and result from effect of environmental stress to oyster.
 
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MDH isozyme;salinity;pH;temperature;PAGE;oyster;
 
ȯ°æ»ý¹° / v.17, no.2, 1999³â, pp.209-215
Çѱ¹È¯°æ»ý¹°ÇÐȸ
ISSN : 1226-9999
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199911921494729)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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