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Çѱ¹È¯°æ»ý¹°ÇÐȸ / v.27, no.4, 2009³â, pp.384-390
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Ç×Áø±Õ¼º Ȱ¼º Bacillus sp.ÀÇ ºÐ¸® ¹× »ý»ê Á¶°Ç
( Isolation and Cultural Conditions of an with Antifungal Activity Bacillus sp. ) |
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| »õ·Î¿î Ç×Áø±Õ¼º Ç×»ý¹°Áú°³¹ßÀ» ¸ñÀûÀ¸·Î °íÃßźÀúº´±ÕÀÎ Colletotrichum gloeosporioides¿¡ ´ëÇÑ Ç×»ýȰ¼ºÀ» °¡Áö´Â ¹Ì»ý¹°À» Åä¾çÀ¸·ÎºÎÅÍ ºÐ¸®ÇÏ¿© µ¿Á¤ÇÑ °á°ú, Bacillus sp. CJ-1·Î µ¿Á¤ÇÏ¿´´Ù. Ç×»ý¹°ÁúÀÇ »ý»êÀ» À§ÇÑ ¹è¾çÁ¶°ÇÀ» °ËÅäÇÑ °á°ú, glucose 1.5% (w/v), yeast extract 0.5% (w/v), pH 6.0ÀÇ ¹èÁö¸¦ »ç¿ëÇÏ¿© $35^{circ}C$¿¡¼ 72½Ã°£ ÁøÅÁ ¹è¾çÇÏ¿´À» ¶§ Ç×Áø±ÕȰ¼ºÀÌ °¡Àå ÁÁ¾ÒÀ¸¸ç, À̶§ Çǰ˱ÕÀÎ C. gloeosporioides¿¡ ´ëÇÑ ¹è¾ç¾×ÀÇ »ýÀ°ÀúÁöȯÀº ¾à 27.8mm·Î ÃÖ´ëÀÇ È°¼ºÀ» º¸¿©ÁÖ¾ú´Ù. Çǰ˱ÕÀÎ C. gloeosporioides¿¡ ´ëÇÑ ¹è¾ç¾×ÀÇ »ýÀ°ÀúÁöÇö»óÀ» Çö¹Ì°æÀ» ÅëÇØ °üÂûÇÑ °á°ú ¹è¾ç¾× $12.5;{mu}L;mL^{-1}$ÀÌ»óÀÇ ³óµµ¿¡¼ ¶Ñ·ÇÇÑ »ýÀ°ÀúÇØÇö»óÀ» °üÂûÇÒ ¼ö ÀÖ¾úÀ¸¸ç ÀÌ´Â ÇÇ°Ë±Õ Æ÷ÀÚÀÇ ¹ß¾Æ¾ïÁ¦Çö»ó°ú ¿¬°üµÇ¾î ÀÖÀ» °ÍÀ¸·Î ÃßÃøµÈ´Ù. |
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| An antifungal antibiotic-producing bacterium was isolated from soil and identified as Bacillus sp. CJ-1. The culture supernatant was found to have a strong and stable antifungal activity against Colletotrichum gloeosporioides. Culture conditions for the maximum antifungal activity were examined. Glucose and yeast extract were selected as the best carbon and nitrogen sources. The optimum C/N ratio was 3. The optimum temperature and initial pH were determined as $35^{circ}C$ and 6.0, respectively. Under these conditions, the production for the antibiotic was maximized at 72 hr at $35^{circ}C$ after cultivation. Microscopic observation showed that the culture supernatant of Bacillus sp. CJ-1 had a strong inhibitory activity on the mycelial growth of the test strain at above $12.5;{mu}L;mL^{-1}$ of concentration. |
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| antifungal;Bacillus sp.;Colletotrichum gloeosporioides; |
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ȯ°æ»ý¹° / v.27, no.4, 2009³â, pp.384-390
Çѱ¹È¯°æ»ý¹°ÇÐȸ
ISSN : 1226-9999
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200920460307091)
¾ð¾î : Çѱ¹¾î |
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| ³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø |
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