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Çѱ¹È¯°æ»ý¹°ÇÐȸ / v.18, no.2, 2000³â, pp.263-268
$Cd^{2+}$ÀÌ ´ßÀÇÀåÇ®ÀÇ ±â°ø°³Æó¿¡ ¹ÌÄ¡´Â ¿µÇâ
( The Effect of $Cd^{2+}$ on Stomatal Apertures of Epidermal Strips in Commelina communis L. )
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$Cd^{2+}$ÀÌ ´ßÀÇÀåÇ®ÀÇ ºÐ¸®Ç¥ÇÇ¿Í ¿ÂÀüÇÑ ÀÙÀÇ ±â°ø°³Æó¿¡ ¹ÌÄ¡´Â ¿µÇâÀ» Á¶»çÇÏ¿´´Ù. $Cd^{2+}$À» ºÐ¸®Ç¥ÇÇ¿¡ ó¸®ÇÏ¿´À» ¶§ ±â°ø¿­¸²ÀÌ ÃËÁøµÇ¾ú´Ù. 100$mu$M$Cd^{2+}$󸮽à ±â°øÀº 8.38$mu extrm{m}$±îÁö ¿­·ÈÀ¸³ª, ´ëÁ¶±¸´Â 3.74$mu extrm{m}$¿´´Ù. ´ëÁ¶±¸ÀÇ ±â°ø Å©±â´Â ÀϹÝÀûÀ¸·Î ÀÛ°Ô ¿­·È´Ù. µû¶ó¼­, ºÐ¸®Ç¥Çǰ¡ »ý¸®ÀûÀ¸·Î °Ç°­ÇÑÁö¸¦ Á¶»çÇϱâ À§ÇØ, »ì¸®½Ç¸¯»ê(SA)°ú ¾Û½Ã½º»ê(ABA)À» ºÐ¸®Ç¥ÇÇ¿¡ ó¸®ÇÏ¿© ±â°øÅ©±â¸¦ ÃøÁ¤ÇÏ¿´´Ù. 100$mu$M SA¿Í 1$mu$M ABA´Â ±â°ø¿­¸²À» °¢°¢ 14%¿Í 28% ¾ïÁ¦ÇÏ¿´´Ù. ºñ½ÁÇÑ È¿°ú°¡ ´Ù¸¥ Á߱ݼÓÀÎ $Al^{3+}°ú Ag^{2+}$¿¡¼­µµ ¹ß°ßµÇ´ÂÁö À̵鿡 ´ëÇÑ ±â°ø°³Æó È¿°ú¸¦ »ìÆìº¸¾Ò´Ù. $Al^{3+}°ú Ag^{2+}$µµ ±â°ø¿­¸²À» °¢°¢ 19%, 41%ÃËÁøÇÏ¿´´Ù. Ä«µå¹Å¿¡ ÀÇÇÑ ±â°ø¿­¸² ±âÀÛÀ» ÀÌÇØÇϱâ À§ÇØ K+Èí¼ö¿¡ ´ëÇÑ Ä«µå¹ÅÀÇ È¿°ú¸¦ Á¶»çÇÏ¿´´Ù. $Cd^{2+}$, SA ±×¸®°í ABA´Â ºÐ¸®Ç¥ÇÇ¿¡¼­ K+Èí¼ö¸¦ °¢°¢ 98%, 28%, 34% ¾ïÁ¦ÇÏ¿´´Ù. $Cd^{2+}$ÀÌ ¿ÂÀüÇÑ ÀÙ¿¡ ¹ÌÄ¡´Â ±â°ø°³ÆóÀÇ ¿µÇâÀ» Á¶»çÇϱâ À§ÇØ 3ÁÖ°£ ¼ºÀåÇÑ ´ßÀÇÀåÇ®À» Hoagland¿ë¾×(0.5mM $Cd^{2+}$, 10mM $Cd^{2+}$)¿¡¼­ ¼ö°æ ¹è¾çÇÑ ÈÄ ¿ÂÀüÇÑ ÀÙÀÇ ±â°øÀüµµµµ¸¦ ÃøÁ¤ÇÏ¿´´Ù. ±× °á°ú, ±â°øÀüµµµµ´Â ´ëÁ¶±¸¿¡ ºñÇØ 5mM $Cd^{2+}$°ú 10mM Cd$^{2+}$¿¡¼­ °¢°¢ 51%, 70%°¨¼ÒµÇ¾ú´Ù. µû¶ó¼­, $Cd^{2+}$Àº ´ßÀÇÀåÇ®ÀÇ ºÐ¸®Ç¥ÇÇ¿Í ¿ÂÀüÇÑ ÀÙÀÇ ±â°ø°³Æó¿¡ »ó¹ÝµÈ ¹ÝÀÀÀ» º¸À̸ç, ºÐ¸®ÇÑ Ç¥ÇÇ¿¡¼­ $Cd^{2+}$¿¡ ÀÇÇÑ ±â°ø¿­¸²Àº $Cd^{2+}ÀÌ K^+$ Èí¼ö¸¦ ¹æÇØÇϰí, ÀÚ½ÅÀÌ °øº¯¼¼Æ÷·Î µé¾î°¡ ÆØ¾ÐÀ» À¯µµÇÑ °á°ú·Î »ç·áµÈ´Ù.
The effect of $Cd^{2+}$ on stomatal apertures of epidermal strips and intact leaves in Commelina communis L. was investigated. Cadmium stimulated stomatal opening. The stomata, treated with 100 $mu$M $Cd^{2+}$ opened to a degree of about 8.38 fm, but the stomata, treated with no cadmium opened to 3.74 ${mu}{ extrm}{m}$. In order to show that there was no mechanical or osmotic impediment preventing the stomata in the epidermal strips, salicylic acid (SA) and abscisic acid (ABA) were used. The treatment of 100 $mu$M SA and 1 $mu$M ABA inhibited 14% and 28% of stomatal opening, respectively. Other heavy metals such as $Al^{3+} and Ag^{2+}$ were also used to investigate the effect of the stomatal apertures. The treatment of $Al^{3+} and Ag^{2+}$ also stimulated 19% and 41% of stomatal opening. To understand how cadmium open stomata, the effect of cadmium on the K+ influx into the epidermal strips was investigated. $Cd^{2+}$, SA, ABA inhibited 98%, 28%, 34% of K+ uptake respectively. 3-weeks old Commelina was transferred to and grown in Hoagland solution (0,5 mM $Cd^{2+}$, 10 mM $Cd^{2+}$) for 4 days and stomatal conductance were measured. The treatment of 5 mM $Cd^{2+}$ and 10 mM $Cd^{2+}$ showed about 51% and 70% inhibition of stomatal conductance, respectively Therefore, it could be concluded that stomata in epidermal strips and intact leaves behaved differently and cadmium- stimulated stomatal opening was due to the result of cadmium uptake into the epidermal strips instead of K+. [cadmium, stomata, stomatal conductance]
 
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ȯ°æ»ý¹° / v.18, no.2, 2000³â, pp.263-268
Çѱ¹È¯°æ»ý¹°ÇÐȸ
ISSN : 1226-9999
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200011921491823)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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