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Çѱ¹È¯°æ»ý¹°ÇÐȸ / v.18, no.2, 2000³â, pp.205-213
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Åä¾ç ¹× ¼ö°èȯ°æ¿¡¼ Transformation¿¡ ÀÇÇÑ ¼¼±Õµé°£ÀÇ ¼öÆòÀû À¯Àü¹°Áú ÀüÀÌ
( Horizontal Gene Transfer among Bacteria by Transformation in Soil and Aquatic Environments ) |
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| ½ÇÇè½Ç¿¡¼ ÇüÁúÀüȯµÉ ¼ö ÀÖ´Â ¼¼±ÕµéÀº ÀÚ¿¬È¯°æ Á¶°Ç¿¡¼µµ ÇüÁúÀüȯ ´É·ÂÀÌ ¹ß´ÞÇÏ´Â °ÍÀ¸·Î ¾Ë·ÁÁ® ÀÖ´Ù. µû¶ó¼, ȯ°æ ³»¿¡¼ ÇüÁúÀüȯ ´É·ÂÀÌ ÀÖ´Â ¼¼±ÕÀÇ Á¸Àç´Â È®½ÇÇÑ °ÍÀ¸·Î ¿©°ÜÁø´Ù. DNA´Â ¹«±â¹°¿¡ ºÎÂøµÈ »óÅ¿¡¼´Â ÇÙ»êºÐÇØÈ¿¼Ò¿¡ ÀÇÇÑ ºÐÇØ·ÎºÎÅÍ º¸È£µÇ´Â °ÍÀ¸·Î ¾Ë·ÁÁ® ÀÖ´Ù. ºñ·Ï DNA°¡ Åä¾ç ¼Ó¿¡ ºÐ»êµÇ¾îÁ® ÀÏÁ¤ ºñÀ²·Î °¡¼öºÐÇØµÇ´õ¶óµµ, ¼ö ÁÖÀÏ ÈÄ¿¡µµ ³·Àº ºñÀ²·Î °¨ÁöµÉ ¼ö ÀÖ´Ù. µû¶ó¼ free DNA´Â ÀÚ¿¬Àû ÇüÁúÀüȯÀ» ÇÒ ¼ö ÀÖÀ» ¸¸Å ÃæºÐÈ÷ Áö¼ÓµÉ ¼ö ÀÖ´Ù. ½ÇÇè½Ç Á¶°Ç¿¡¼´Â ¼¼±ÕÀÇ ÇüÁúÀüȯÀÌ ¿©·¯ °æ¿ì º¸°íµÇ¾úÁö¸¸, ÀÚ¿¬»óÅ¿¡¼ ÇüÁúÀüȯ°ú °ü·ÃµÈ ÀÚ·á´Â ¸Å¿ì Àû´Ù. »ýÅÂÇÐÀûÀ¸·Î GMMs·ÎºÎÅÍ ÀçÁ¶ÇÕ DNA°¡ ÅäÂø ¹Ì»ý¹°¿¡ ÀüÀÌµÉ ¼ö ÀÖ´Â ÀáÀç·Â¿¡ ´ëÇÑ ¹®Á¦°¡ ÁÖ¿ä Çö¾ÈÀÌ µÇ¾ú´Âµ¥, ÀÌ´Â ÀüÀÌµÈ DNA°¡ ¹æÃâµÈ ¼¼±ÕÀÇ »ýÅÂÇÐÀûÀÎ ÀûÀÀ·ÂÀ» º¯È½ÃÄÑ »ý¹°ÇÐÀû ¾ÈÀü¼ºÀÇ ¹®Á¦¸¦ ¾ß±âÇÒ ¼ö Àֱ⠶§¹®ÀÌ´Ù. ¹°·Ð, ¹æÃâµÈ GMMs·ÎºÎÅÍ ÀçÁ¶ÇÕ DNA°¡ ÅäÂø ¹Ì»ý¹°¿¡ ÀüÀ̵Ǵ À²Àº ¾ÆÁÖ ³·Àº ºóµµ·Î ÀϾÁö¸¸, ºóµµ°¡ ³·´Ù´Â °ÍÀº ±×¸® Áß¿äÇÏÁö ¾Ê´Ù. ¿Ö³ÄÇϸé, ºñ·Ï ³·Àº ºóµµ·Î ÀüÀ̵Ǵõ¶óµµ À¯¸®ÇÑ Á¶°ÇÀ» ¸¸³ª°Ô µÇ¸é ÀüÀÌµÈ À¯ÀüÀÚ´Â ¼±ÅÃµÉ ¼ö Àֱ⠶§¹®ÀÌ´Ù. ÀÌÁ¦±îÁö GMMs´Â ½ÇÇè½ÇÀ̳ª Á¦ÇÑµÈ È¯°æ¿¡¼ ÁÖ·Î »ç¿ëµÇ¾úÁö¸¸ ¾ÕÀ¸·Î´Â °³¹æµÈ ÀÚ¿¬ »ýŰ迡¼ ÀÌ·ç¾îÁú Àü¸ÁÀÌ´Ù. ±×·¯¹Ç·Î GMMs°¡ ÅäÂø¼¼±Õ¿¡ ¹ÌÄ¡´Â ¿µÇâ¿¡ ´ëÇØ¼µµ ¿¬±¸µÇ¾î¾ß ÇÏ°í µ¿½Ã¿¡ GMMs°¡ »ýŰ迡 ¹æÃâµÉ °æ¿ì ±×¿¡ µû¸¥ ¿µÇâÆò°¡¸¦ ¹Ýµå½Ã ¼öÇàÇØ¾ß ÇÑ´Ù. |
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| Laboratory studies have revealed that naturally transformable bacteria develop competence under in situ conditions. Thus, the occurrence of competent bacteria in the environment can be considered as a certainty The persistence of free DNA in natural habitats is influenced by nucleolytic degradation and protection from degradation by adsorption to minerals. Although DNA seeded into natural environment was hydrolysed at substantial rates, but was still detectable at low levels after even several weeks. Compared to the number of laboratory based studies, only a few data have been published dealing with transformation of bacteria in the field. Recently, the potential transfer of recombinant DNA (rDNA) from deliberately or accidentally released bacteria to indigenous microbes has raised biosafety issues, since the persistence of rDNA becomes independent of the survival of its original host and leads to unpredictable, long-term ecological effects. The aim of the present review is to summarise recent literature on horizontal gene transfer (HGT) by transformation among bacteria in both soil and aquatic habitat and special emphasis is placed on recent reports which have addressed HGT among bacteria in the field. [Transformation, Horizontal gene transfer (HGT), recombinant DNA (rDNA), Genetically modified microorganisms (GMMs), Biosafety] |
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ȯ°æ»ý¹° / v.18, no.2, 2000³â, pp.205-213
Çѱ¹È¯°æ»ý¹°ÇÐȸ
ISSN : 1226-9999
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200011921491755)
¾ð¾î : Çѱ¹¾î |
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| ³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø |
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