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Çѱ¹È¯°æ»ý¹°ÇÐȸ / v.19, no.1, 2001³â, pp.79-86
Á¦ÁÖµµ ¹ÙÁö¶ô¿¡ ±â»ýÇÏ´Â ¹ÙÁö¶ôÆ÷ÀÚÃæ, Perkinsus sp.ÀÇ °ø°£Àû ºÐÆ÷ ¹× Á¶Á÷ÇÐÀû °üÂû
( Histopathology and Spatial Distribution of the Protozoan Parasite, Perkinsus sp. Found in the Manila Clam Ruditapes philippinarum Distributed in Cheju, Korea )
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¹ÙÁö¶ôÆ÷ÀÚÃþ Perkinsus´Â Apicomplexa ¹®¿¡ ¼ÓÇÏ´Â ±â»ý¼º ¿ø»ýµ¿¹°·Î¼­ À¯·´¿¡¼­´Â ¹ÙÁö¶ôÀÇ ´ë·®Æó»ç ¿øÀÎ »ý¹°·Î Àß ¾Ë·ÁÁ® ÀÖÀ¸¸ç ¿ì¸®³ª¶ó¿¡¼­µµ 90³â´ë ÃʹݺÎÅÍ ³ªÅ¸³ª°í ÀÖ´Â ¹ÙÁö¶ô Æó»çÀÇ ¿øÀλý¹°·Î ÃßÁ¤µÇ°í ÀÖ´Ù. ÀÌ ¿¬±¸´Â ¿ì¸®³ª¶ó ¹ÙÁö¶ôÆ÷ÀÚÃæ °¨¿°ºÐÆ÷ Á¶»çÀÇ ÀÏȯÀ¸·Î Á¦ÁÖ ¿¬¾È¿¡ ¼­½ÄÇÏ´Â ¹ÙÁö¶ôÀÇ ¹ÙÁö¶ôÆ÷ÀÚÃæ °¨¿° ÇöȲ°ú °¨¿°µµ, Á¶Á÷º´¸®ÇÐÀû Çö»ó, À¯ÁÖÀÚ Çü¼º ±×¸®°í ¼­½ÄÁöÀÇ ÅðÀû¹° Á¶¼º¿¡ µû¸¥ °¨¿° Ư¼ºÀ» Á¶»çÇϱâ À§ÇÏ¿© ½Ç½ÃµÇ¾ú´Ù. RayÀÇ Fluid Thioglycollate Medium (FTM) ¹æ¹ý°ú ChoiÀÇ NaOH lysis ¹æ¹ýÀ» ÀÌ¿ëÇÑ Áø´Ü°á°ú ±è³çÇ×°ú ¿ë¸Ó¸® Áö¿ªÀÇ ¹ÙÁö¶ôÀº ºñ°¨¿° »óÅ¿´À¸¸ç, À̵é Áö¿ªÀ» Á¦¿ÜÇÑ Áö¿ªÀÇ ¹ÙÁö¶ôÆ÷ÀÚÃæÀÇ ¹ßÇöÀ²Àº Ç¥¼± 100%, ¼º»êÇ× 70%, ±Ý´É 63%, Á¾´Þ¸® 33%, ÀÌÈ£ 21%, ¸ð½½Æ÷ 17%,¼­±ÍÆ÷ 14%, ¼øÀ¸·Î ³ªÅ¸³µ´Ù. ¹ÙÁö¶ô ½ÀÁß·® 1g´ç Ç¥¼± 98,430 ¼¼Æ÷, ¼º»ê 78,553 ¼¼Æ÷, ±Ý´É 18,980 ¼¼Æ÷, Á¾´Þ¸® 4,290 ¼¼Æ÷, ÀÌÈ£ 1,527 ¼¼Æ÷, ¸ð½½Æ÷ 1,069 ¼¼Æ÷, ¼­±ÍÆ÷ 853 ¼¼Æ÷ÀÇ ¹ÙÁö¶ôÆ÷ÀÚÃæÀÌ °ËÃâµÇ¾ú´Ù. Á¶Á÷º´¸®ÇÐÀû °Ë»ç°á°ú Á÷°æ 5-10$mu extrm{m}$ÀÎ ¿øÇüÀÇ trophozoite°¡ ¹ÙÁö¶ôÀÇ ¼ÒÈ­¸Í³¶°ú ¾Æ°¡¹Ì¿¡ ºÐÆ÷Çϰí ÀÖÀ½ÀÌ È®ÀεǾúÀ¸¸ç ¼÷ÁÖÀÇ Ç÷±¸ÁýÁß Çö»óµµ °üÂûµÇ¾ú´Ù. À¯ÁÖÀÚ´Â FTM¿¡¼­ 2Àϵ¿¾È ¹è¾çµÈ hypnospore¸¦ $25^{circ}C$ÀÇ ÇØ¼ö¿¡¼­ 2Àϰ£ ¹è¾çÇÏ¿´À» ¶§ ¼¼Æ÷ºÐ¿­À» °ÅÃÄ ¹æÃâ°üÀ» ÅëÇÏ¿© ¹æÃâµÆ´Ù. ÀÌ»óÀÇ Á¶»ç °á°ú Á¦ÁÖ ¿¬¾È¿¡ ¼­½ÄÇϰí ÀÖ´Â ´ëºÎºÐÀÇ ¹ÙÁö¶ôÀÌ ¹ÙÁö¶ôÆ÷ÀÚÃæ¿¡ °¨¿°µÇ¾î ÀÖÀ½ÀÌ È®ÀεǾú´Ù. ±×·¯³ª ¿ì¸®³ª¶ó ³²¤ý¼­Çؾȿ¡¼­ º¸°íµÈ ¹ÙÁö¶ô Æ÷ÀÚÃæ °¨¿°À²À̳ª °¨¿°µµ º¸´Ù´Â ¸Å¿ì ³·Àº ¼öÁØÀ̾ú´Ù. ÅëÇØ ±â¹Ð¼º Ä§ÇØÀÇ ¿ì·Á°¡ ÀÖ´Â ¸Þ½ÃÁö ¸í·É¾î¸¦ Â÷´ÜÇϰí, ±â´É ¸í·É¾î Â÷´Ü¿¡ ÀÇÇÑ ÀÚ¿øÀÇ ±â¹Ð¼ºÀ» ÇØ°áÇÏ¿´´Ù.¼º ´ÙÇÑÁõ ¹ß»ýÀ» ºñ±³ÇÑ °á°ú ±³Åë°¡Áö ÀýÁ¦¼ú¿¡¼­ º¸»ó¼º ´ÙÇÑÁõ ¹ß»ýÀÇ À¯ÀÇÇÑ °¨¼Ò¸¦ º¸¿© º¸»ó¼º ´ÙÇÑÁõÀ» °¨¼Ò½Ã۱â À§Çؼ­´Â ±³Åë°¡Áö ÀýÁ¦¼úÀÌ ¿ì¼öÇÔÀ» ¾Ë ¼ö ÀÖ¾ú´Ù.°Ô ³ªÅ¸³µ´Ù. ±×·¯¹Ç·Î ½Äµµ¿­°øÀ» ÅëÇÑ ½ÄµµÀýÁ¦¼úÀº ÁÖº¯ Àå±â¿¡ ħ¹üÀÌ ¾ø°í ¾ÏÀÇ ±æÀ̰¡ ºñ±³Àû ª°í ¼ú Àü Àü½Å»óŰ¡ ÁÁÁö ¾Ê¾Æ È£Èí±â°è ÇÕº´Áõ µîÀÌ ¿ì·ÁµÇ´Â ȯÀÚ¿¡°Ô ¼±ÅÃÀûÀ¸·Î ½ÃÇàÇÑ´Ù¸é ÁÁÀº °á°ú¸¦ ¾òÀ» ¼ö ÀÖÀ¸¸®¶ó »ç·áµÈ´Ù. ±×¸®°í µÎ ¼ú½ÄÀº Áýµµ¿Ü°£ÀÇ ±âÈ£¿Í °æÇè¿¡µµ Á¿ìµÉ ¼ö ÀÖ´Ù°í ÇϰڴÙ.<0.05), ¸é·ù(p<0.01)¿Í Á¾·ùº°·Î´Â µ¤¹ä, Â¥Àå¹ä(p<0.01), ÇØÀå±¹, ÄŶó¸é(p<0.05) µî¿¡ ´ëÇØ À¯ÀÇÇÑ Â÷À̰¡ ÀÖ¾úÀ¸¸ç ¸ðµÎ 26¼¼ ÀÌ»óÀÌ °¡Àå ¸¹ÀÌ ÀÌ¿ëÇÏ´Â °ÍÀ¸·Î Á¶»çµÇ¾ú´Ù. Áï¼®°¡°ø½ÄǰÀ» Á¢ÇÏ°Ô µÈ µ¿±â´Â 'TV±¤°í', 'È£±â½É ', 'Ä£±¸/°¡Á·µéÀÇ ±ÇÀ¯¿Í ¼Ò°³', '½Å¹®À̳ª ÀâÁö±¤°í', 'ÀÎÅͳݱ¤°í ¹× È«º¸' µîÀÇ ¼øÀ̾úÀ¸¸ç, Áï¼®°¡°ø½ÄǰÀÇ ±¸¸ÅÀå¼Ò·Î´Â 'ÆíÀÇÁ¡'À» °¡Àå ¸¹ÀÌ ÀÌ¿ëÇÏ¿´°í ±× ´ÙÀ½À¸·Î 'µ¿³× Àαٽ´ÆÛ', '´ëÇü¸¶Æ®', '¹éÈ­Á¡ ' µîÀÇ ¼øÀ¸·Î ³ªÅ¸³µ´Ù. À§ÀÇ °á°ú·Î º¼ ¶§ Áï¼®°¡°ø½ÄǰÀÇ ÀÌ¿ë·üÀÌ °è¼ÓÀûÀ¸·Î Áõ´ëµÇ°í ÀÖÀ¸³ª ³²ÀÚ°¡ ¿©ÀÚº¸´Ù ¶Ç´Â ºñÁ¶¸®Àü°øÇлýÀÌ Á¶¸®°ü·ÃÀü°ø Çлýº¸´Ù Áï¼®°¡°ø½ÄǰÀ» ¼±È£ÇÏ´Â °ÍÀ¸·Î ³ªÅ¸³ª ¼¼ºÐÈ­ °í°´¿¡ ´ëÇÑ Á¦Ç° °³¹ß°ú È«º¸¹æ¾ÈÀ» °í·Á ÇØ º¸¾Æ¾ß ÇÒ °ÍÀ¸·Î »ç·áµÈ´Ù ¶ÇÇÑ ´ëÇлýµéÀº ÀϺΠÁï¼®°¡°ø ½Äǰ¿¡ ´ëÇØ¼­ »ó´ëÀûÀ¸·Î ±âÈ£µµ¿Í À̿뵵°¡ ³ôÀº °ÍÀ¸·Î ³ªÅ¸³µ´Âµ¥, ´ëÇлýµéÀÇ ÀÎÅÍ³Ý »ç¿ëÀ²ÀÌ ³ôÀº Á¡À» ÀÌ¿ëÇÑ ÀÎÅͳݻóÀÇ Àû±ØÀûÀÎ ±¤°í¿Í ´Ù¾çÇÑ ¸Þ´º °³¹ß°ú È«º¸¸¦ À§ÇÑ ²ÙÁØÇÑ ³ë·ÂÀÌ ÇÊ¿äÇÒ °ÍÀ¸·Î º»´Ù. ±×·¯³ª, º» ¿¬±¸¿¡¼­´Â Ç¥º»ÀÌ °ü±¤°ü·Ã Àü°øÀÇ ´ëÇлýÀ¸·Î ÆíÁߵǾúÀ¸¹Ç·Î, ÀÌ
The apicomplexan parasite, Perkinsus sp., has been reported as a causative pathogen responsible for the mass mortality of the Manila clams, Ruditapes philippinarum in European countries as well as in Korea. Prevalence, infection intensity, histopathologic features and zoosporulation of Perkinsus found in the Manila clams distributed along the coast of Cheju Island were investigated in this study. Clams were collected from nine different beaches along the Cheju Island from May to July in 2000. Ray's Fluid Thioglycollate Medium (RFTM) and Choi's NaOH lysis methods were applied in the diagnosis and quantification of the Perkinsus infection. Prevalence, percentage infection of the parasite examined was 100% in Pyosun, 70% in Sungsan, 63% in Kumneong, 33% in Jongdalri, 21% in Iho, 17% in Moslpo, and 14% in Seogwipo. No Perkinsus was found in the clams collected from Kimnyong and Yongmeo-ri. Infection intensity as a number of Perkinsus cells per gram tissue wet weight (twwt), was 98,430 cells/g twwt in Pyosun, 78,553 cells/g twwt in Sungsan, 18,980 cells/g twwt in Kumneong, 4,290 cells/g twwt in Jongdalri, 1,527 cells/g twwt in Iho, 1,069 cells/g twwt in Moslpo, and 853 cells/g twwt in Seogwipo. Histological preparation of the infected tissues revealed trophozoites of Perkinsus sp., ranged from 5 to 10 ${mu}{ extrm}{m}$, in diameter mostly distributed in the digestive gland and the gill filaments. Zoospores were discharged from the hypnospore via discharging tube about 2 days after incubated in filtered and aerated seawater. In general, the prevalence and infection intensity of Perkinsus in Cheju Island were much lower than that reported from the western and southern coast of Korea.
 
Ű¿öµå
Perkinsus;Manila clam;Ruditapes philippinarum;
 
ȯ°æ»ý¹° / v.19, no.1, 2001³â, pp.79-86
Çѱ¹È¯°æ»ý¹°ÇÐȸ
ISSN : 1226-9999
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200111921492183)
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