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Çѱ¹È¯°æ»ý¹°ÇÐȸ / v.20, no.1, 2002³â, pp.85-90
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( Effects of Acidification on the Species Composition and the Changes of Extracelluar Enzymes of Heterotrophic Bacterial Community )
ÃÖ¿µ±æ;ÇѸí¼ö;±è¼¼È­;À̰æ;À¯±¤ÀÏ; ÇѾç´ëÇб³ ÀÚ¿¬°úÇдëÇÐ »ý¸í°úÇаú;ÇѾç´ëÇб³ ÀÚ¿¬°úÇдëÇÐ »ý¸í°úÇаú;¿ëÀδëÇб³ »ý¸í°úÇаú;°¡Å縯´ëÇб³ »ý¹°ÇкÎ;ÇѾç´ëÇб³ ÀÚ¿¬°úÇдëÇÐ »ý¸í°úÇаú;
 
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ÀÎÀ§ÀûÀ¸·Î pH ±¸¹è°¡ Á¶¼ºµÈ ȸºÐ¹è¾ç ½Ã½ºÅÛ (batch culture system)³»¿¡¼­ »ê¼ºÈ­¿¡ µû¸¥ Á¾¼Ó¿µ¾ç ¼¼±Õ ±ºÁýÀÇ Á¾Á¶¼º º¯È­¸¦ ºÐ¼®ÇÏ¿´´Ù. ÃѼ¼±ÕÀÇ °³Ã¼¼ö´Â »ê¼ºÈ­¿¡ ¿µÇâÀ» ¹ÞÁö ¾Ê¾ÒÀ¸¸ç, Á¾¼Ó¿µ¾ç¼¼±Õ ±ºÁýÀÇ Å©±â´Â pH°¡ ³·¾ÆÁú¼ö·Ï °¨¼ÒÇÏ¿´´Ù. Àü pH±¸°£¿¡¼­ ºÐ¸®µÈ Á¾¼Ó¿µ¾ç ¼¼±ÕµéÀº ¸ðµÎ 12¼Ó 22Á¾À¸·Î ³ªÅ¸³µÀ¸¸ç, ÀÌÁß ±×¶÷À½¼º ¼¼±ÕÀº 64%,±×¶÷¾ç¼º ¼¼±ÕÀº 36%ÀÇ ºñÀ²·Î ºÐÆ÷ÇÏ¿´´Ù. pH°¡ ³·¾ÆÁü¿¡ µû¶ó ±×¶÷¾ç¼º ¼¼±ÕÀÇ ºÐÆ÷ ºñÀ²Àº °¨¼ÒÇÏ´Â ¹Ý¸é, ±×¶÷À½¼º ¼¼±ÕÀÇ ºÐÆ÷ ºñÀ²Àº Áõ°¡ÇÏ´Â ¾ç»óÀ» º¸¿´´Ù. °¢ pH ±¸°£º° ¼ÓÀÇ ºÐÆ÷ ºñÀ²Àº pH 7¿¡¼­´Â 12°³ ¼ÓÀÌ ÃâÇöÇÏ¿´À¸³ª, pH 3¿¡¼­´Â 5°³ ¼Ó¸¸ ÃâÇöÇÏ¿© pH°¡ ³·¾ÆÁü¿¡ µû¶ó ¼ÓÀÇ ´Ù¾ç¼ºÀÌ °¨¼ÒÇÏ´Â ¾ç»óÀ» ³ªÅ¸³»¿´´Ù. ¼¼Æ÷¿Ü È¿¼ÒÀÇ È°¼ºµµ´Â $0.008-0.292;mu{M}ell^{-1};hr^{-1}$ÀÇ ¹üÁÖ·Î ³ªÅ¸³µ°í, »ê¼ºÈ­¿¡ µû¶ó ¼¼Æ÷¿Ü È¿¼ÒÀÇ È°¼ºµµ´Â ±Þ°ÝÈ÷ °¨¼ÒÇÏ¿© pH 5ÀÌÇÏ¿¡¼­´Â 5-38%ÀÇ ³·Àº Ȱ¼ºÀ» ³ªÅ¸³»¾ú´Ù.
In an artificial pH-gradient batch culture system, the author analyzed the effects of acidification on the species composition of heterotrophic bacteria. As the result of this study, it was found that the numbers of total bacteria were not affected by acidification and that the population size of heterotrophic bacteria decreased as pH became lower. The heterotrophic bacteria isolated from all of the pH gradient were 12 genera and 22 species, and among them, gram negative and gram positive bacteria were 04% and 30%, respectively. As pH decreased, the distribution rate of gram negative bacteria increased while that of gram positive bacteria decreased. Regarding to distribution rate of genuses in each pH gradient, 13 genuses appeared at pH 7 while only 5 genuses appeared at pH 3, which means that the diversity of genera decrease as pH decreased. The activities of extracellular enzyme showed the ranges of $0.008-0.292;mu{M}ell^{-1};hr^{-1}$ in bioreactor system. The enzymatic activities decreased rapidly below pH 5 and then sustained 5-38% at the lower pH values.
 
Ű¿öµå
acidification;heterotrophic bacteria;pH;extracellular enzyme;
 
ȯ°æ»ý¹° / v.20, no.1, 2002³â, pp.85-90
Çѱ¹È¯°æ»ý¹°ÇÐȸ
ISSN : 1226-9999
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200211921492534)
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