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Çѱ¹È¯°æ»ý¹°ÇÐȸ / v.20, no.3, 2002³â, pp.232-236

( Toxicity Decrease of Cadmium by the Pigment Produced by Azomonas agilis PY101 in the Culture of Bacterial Cells and Vero Cells )
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The morphological patterns and the cytopathogenicity time of the Vero cells induced by free $Cd^{2+}$ and pigment-bound $Cd^{2+}$ were observed by inverted microscope in order to investigate the difference of cadmium toxicity. The Vero cells induced by Hee $Cd^{2+}$ of 0.1 mM were shown to have a fatal toxic effect and the cytopathogenicity could be seen early after 6$pm$2 hours of incubation. Partially affected cells induced by pigment-bound $Cd^{2+}$ of 0.1 mM were shown and the cytopathogenicity could be seen after 20 hours of incubation. The Vero cells grown with free 0.001 mM $Cd^{2+}$ were also affected and the cytopathogenicity could be seen after 17 hours of incubation, whereas the Vero cells grown with 0.001 mM pigment- bound $Cd^{2+}$ were unaffected. The sensitivity of Escherichia coli DH5$alpha$ bacterial cells was also examined after a short treatment with free $Cd^{2+}$ or pigment-bound $Cd^{2+}$. About 5% of cells survived after 0.01 mM of free $Cd^{2+}$ treatment, while about 68% of cells survived after 0.01 mM of pigment- bound $Cd^{2+}$.
 
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Vero cells;Azomonas agilis;pigment;cadmium;toxicity;
 
ȯ°æ»ý¹° / v.20, no.3, 2002³â, pp.232-236
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ISSN : 1226-9999
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200211921492749)
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