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한국환경생물학회 / v.21, no.1, 2003년, pp.52-55 
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MCF-7 세포주에서$gamma$선에 의한 세포신호 전달 관련 유전자의 발현 양상의 분석
( Signal Transduction-related Gene Expression Analysis in MCF-7 followed by $gamma$-radiation ) |
| 박지윤;황창일;박웅양;김진규;채영규; 한양대학교 생화학 및 분자생물학과;서울대학교 의과대학 생화학교실;서울대학교 의과대학 생화학교실;한국원자력연구소 동위원소 방사선 응용팀;한양대학교 생화학 및 분자생물학과;
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| 초 록 |
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| There is considerable evidence that ionizing radiation (IR) mediates checkpoint control, repair and cell death. In this study, we have used a high density microarray hybridization approach to characterize the transcriptional response of human breast carcinoma MCF-7 cell line to ${gamma}$-radiation, such as 4 Gy 4 hr, 8 Gy 4 hr, and 8 Gy 12 hr. We found that exposure to ${gamma}$-ray alters by at least a $log_2$ factor of 1.0 the expression of 115 known genes. Of the 66 genes affected by ${gamma}$-radiation, 49 are down-regulated. In our results, the cellular response to irradiation includes induction of the c-jun and EGR1 early response genes. The present work has examined potential cytoplasmic signaling cascades that transduce IR-induced signals to the nucleus. 40S ribosomal protein s6 kinase modulates the activities of the mitogen activated protein kinase (MAPK) and c-Jun $NH_2$-terminal kinase (JNK1) cascades in human monocytic leukemia (U937/pREP4) cells. 14-3-3 family members are dimeric phosphoserine -binding proteins that participate in signal transduction and checkpoint control pathways. |
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| 키워드 |
| irradiation;MCF-7;cDNA microarray;cell signaling; |
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환경생물 / v.21, no.1, 2003년, pp.52-55
한국환경생물학회
ISSN : 1226-9999
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200311921575189)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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