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Çѱ¹È¯°æ»ý¹°ÇÐȸ / v.21, no.3, 2003³â, pp.234-239
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Cd$^{2+}$ ¿¡ ÀÇÇÑ ´ßÀÇÀåÇ®ÀÇ ½Ä¹° µ¶¼º¿¡ Indole acetic acid°¡ ¹ÌÄ¡´Â ¿µÇâ
( The Effects of Indole Acetic Acid (IAA) on Cd$^{2+}$_induced Physiological Toxicities in Commelina communis L. ) |
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| 3ÁÖ°£ Á¤»óÀûÀ¸·Î »ýÀåÇÑ ´ßÀÇÀåÇ®À» Hoagland¿ë¾× ($pm 100 mu M ;Cd^{2+}, 100 mu M ;Cd^{2+}+10 mu M;IAA,100 mu M; Cd^{2+}+100 mu M ; IAA,100 mu M ; Cd^{2+} +1 mu M ; IAA)$¿¡¼ 3ÁÖ°£ ¼ö°æÀç¹è ÇÑ ÈÄ »ýÀå, ±â°ø°³Æó, ¿±·Ï¼Ò ÇÔÀ¯·®, ¿±·Ï¼Ò Çü±¤ ¹× ¼öºÐÆÛÅÙ¼ÈÀ» Á¶»çÇÏ¿´´Ù. Hoagland¿ë¾×¿¡ $Cd^{2+}$ 󸮽à 3ÁÖ µ¿¾È 2.0 cm »ýÀåÇßÀ¸³ª, ´ëÁ¶±¸´Â 7.0 cm»ýÀåÇß´Ù. ¹Ý¸é¿¡ Ä«µå¹Å¿¡ 10$mu$M IAA¸¦ °°ÀÌ Ã³¸®ÇÑ °æ¿ì´Â 3ÁÖ µ¿¾È 3.7 cmÀÚ¶úÀ¸¸ç, 100 $mu$M IAA¸¦ °°ÀÌ Ã³¸®Çϸé 3ÁÖ µ¿¾È 5 cm±×¸®°í 1mM IAAÀÇ °æ¿ì´Â 3.3 cm »ýÀåÇÏ¿´´Ù. $Cd^{2+}$Àº ±â°ø¿¸²À» ÃËÁøÇÏ¿´À¸¸ç, ¾Æ¿ï·¯ IAA÷°¡´Â $Cd^{2+}$À» ´Üµ¶Ã³¸® ÇÑ °æ¿ìº¸´Ù ±â°ø¿¸²À» ´õ ÃËÁøÇÏ¿´´Ù. ¿±·Ï¼Ò ÇÔ·®ÀÇ º¯È´Â $Cd^{2+}$ 󸮱¸¿¡¼ ¶Ñ·ÇÇÏ°Ô °üÂûµÇ¾ú´Ù. Cd$^{2£«}$ 󸮱¸´Â ´ëÁ¶±¸¿¡ ºñÇØ 3ÁÖ ¶§ ¿±·Ï¼Ò ÇÔ·®ÀÌ 13%¾ïÁ¦µÇ¾ú´Ù. $Cd^{2+}$°ú IAA¸¦ ÇÔ²² ó¸®ÇÑ °æ¿ì´Â 10 $mu$M°ú 100 $mu$M¿¡¼´Â ¶Ñ·ÇÇÑ ¿±·Ï¼Ò ÇÔ·®ÀÇ º¯È°¡ °üÂûµÇÁö ¾Ê¾Ò´Ù. 1 mM IAA 󸮱¸¿¡¼´Â ¾à 9% ¿±·Ï¼Ò ÇÔ·®ÀÌ °¨¼ÒÇÏ¿´´Ù. ¸ðµç 󸮱¸¿¡¼ Á¤»óÀûÀÎ ¿±·Ï¼Ò a/bºñÀ²À» º¸¿©ÁÖ¾úÀ¸³ª, $Cd^{2+}$󸮱¸¿¡¼ 3ÁÖ ¹è¾çÇÑ °æ¿ì 2.38À» º¸¿©ÁÖ¾ú´Ù. Fv/Fm ÃøÁ¤ °á°ú $Cd^{2+}$ 󸮴 Fv/Fm°ª¿¡ ¿µÇâÀ» ÁÖ¾ú´Ù. Ä«µå¹Å ó¸® 1ÁÖ¿¡´Â Å« º¯È°¡ ¾ø¾úÀ¸³ª,2ÁÖ¿Í 3ÁÖ Ã³¸®½Ã¿¡´Â ´ëÁ¶±¸¿¡ ºñÇØ °¢°¢ 9%¿Í 11% ¾ïÁ¦µÇ¾ú´Ù. $Cd^{2+}$󸮽à ¾à 3ÁÖ ÈÄ¿¡ ¾à 67%°¡ ¼öºÐÆÛÅÙ¼ÈÀÌ °¨¼ÒÇÏ¿´´Ù. ¹Ý¸é¿¡ $Cd^{2+}$¿¡ IAA¸¦ ´Ù¾çÇÑ ³óµµ¿¡¼ ÷°¡ÇÏ¸é ¼öºÐÆÛÅÙ¼ÈÀÇ º¯È´Â ¹Ì¹ÌÇÏ¿´´Ù. À§ÀÇ °á°úµé·ÎºÎÅÍ IAA´Â ½Ä¹°ÀÇ »ýÀå, ¿±·Ï¼Ò ÇÔ·®,Fm/Fv±×¸®°í ¼öºÐÆÛÅټȿ¡¼ $Cd^{2+}$¿¡ ÀÇÇÑ ½É°¢ÇÑ µ¶¼ºÀ» ¶Ñ·ÇÇÏ°Ô ¿ÏȽÃŰ´Â °ÍÀ¸·Î »ç·áµÈ´Ù. |
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| 3-weeks old Commelina was transferred to and grown in Hoagland solution (($pm 100 mu M ;Cd^{2+}, 100 mu M ;Cd^{2+}+10 mu M;IAA,100 mu M; Cd^{2+}+100 mu M ; IAA,100 mu M ; Cd^{2+} +1 mu M ; IAA)$) for three weeks and then a number of physiological activities was investigated. In the control the length of stem was increased to 7 cm after 3 weeks, but in the treatment of $Cd^{2+}$, 2.0 cm was grown. In cases of IAA with $Cd^{2+}$, the growth of the plants was increased to 3.7 cm, 5.0 cm and 3.3 cm in 100 $mu$M, 10 $mu$M and 1 mM IAA respectively, Cadmium stimulated stomatal opening. The stomata, treated with $Cd^{2+}$ opened to n degree of about 6.1 $mu extrm{m}$, but the stomata, treated with no cadmium opened to 4.5 $mu extrm{m}$. In the treatment of various concentation of IAA with cadmium the stomata opend to about 1 $mu extrm{m}$ more than that of cadmium alone. Cadmium reduced total chlorophyll content up to 13% for 3 weeks. In the treatment of $Cd^{2+}$ £« IAA (10 $mu$M and 100 $mu$M) the clear change of total chlorophyll content was not observed, but in the addition of 1 mM IAA to $Cd^{2+}$ reduced the total chlorophyll content to about 9%. $Cd^{2+}$ reduced to 9% and 11% of Fv/Fm after two and three weeks respectively. In cases of IAA with $Cd^{2+}$, there were no clear changes of Fv/Fm. Cadmium reduced water potential to 67% after 3 weeks incubation, but in cases of IAA with $Cd^{2+}$, water potential was not clearly changed. Therefore, it could be concluded that the treatment of IAA showed clear alleviation of $Cd^{2+}$-induced several physiological toxicities. |
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| cadmium;calcium;indole acetic acid;Commelina communis; |
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ȯ°æ»ý¹° / v.21, no.3, 2003³â, pp.234-239
Çѱ¹È¯°æ»ý¹°ÇÐȸ
ISSN : 1226-9999
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200311921879623)
¾ð¾î : Çѱ¹¾î |
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| ³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø |
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