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Çѱ¹È¯°æ»ý¹°ÇÐȸ / v.22, no., 2004³â, pp.54-62
½Ä¹°ÇöûũſÀÇ ¼ºÀå°ú ±¤ÇÕ¼º¿¡ ´ëÇÑ benzo¡²a¡³pyreneÀÇ ¿µÇâ
( Effects of Benzo¡²a¡³pyrene on Growth and Photosynthesis of Phytoplankton )
±è¼±ÁÖ;½Å°æ¼ø;¹®Ã¢È£;¹Úµ¿¿ø;À常; ºÎ°æ´ëÇб³ ÇØ¾çÇаú;Çѱ¹Çؾ翬±¸¿ø ³²ÇØ¿¬±¸¼Ò ³²ÇØÆ¯¼º¿¬±¸º»ºÎ;ºÎ°æ´ëÇб³ ÇØ¾çÇаú;Çѱ¹Çؾ翬±¸¿ø ³²ÇØ¿¬±¸¼Ò ³²ÇØÆ¯¼º¿¬±¸º»ºÎ;Çѱ¹Çؾ翬±¸¿ø ³²ÇØ¿¬±¸¼Ò ³²ÇØÆ¯¼º¿¬±¸º»ºÎ;
 
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º» ¿¬±¸´Â ÇØ¾ç ½Ä¹°Çöûũſ¿¡ ´ëÇÑ Áö¼Ó¼º À¯±â¿À¿°¹°ÁúÀÇ ¿µÇâÀ» ÀÌÇØÇϱâ À§ÇÑ ¸ñÀûÀ¸·Î, ¿ì¸®³ª¶ó ¿¬¾È¿ª¿¡¼­ ¿¬°£ ¿ìÁ¡ÀûÀ¸·Î ³ªÅ¸³ª´Â ½Ä¹°Çöûũſ ÁÖ¿ä 5Á¾ Áï, ±ÔÁ¶·ù Skeletonema costatun, ħÆí¸ðÁ¶·ù Heterosigma akashiwo, ¿ÍÆí¸ðÁ¶·ù Prorocentrum dentatum, P. minimum, Akashiwo sanguinea¸¦ ÀÌ¿ëÇÏ¿© benzo¡²a¡³pyrene(PAHs)¿¡ 72½Ã°£µ¿¾È ³ëÃâ½ÃŲ ´ÙÀ½ °¢ Á¾ÀÇ ¼ºÀå ¹× ÀúÇØ, ȸº¹´É·Â µîÀ» Á¶»çÇÏ¿´°í, benzo¡²a¡³pyreneÀÇ ³óµµ 0.1, 1, 5, 10 $mu extrm{g}$ L$^{-1}$¿¡ H. akashiwo (Raphidophyceae)¸¦ ³ëÃâ½ÃÄÑ ½Ã°£¿¡ µû¸¥ ±¤ÇÕ¼º·üÀÇ º¯È­¸¦ ÃøÁ¤ÇÏ¿´´Ù. Benzo¡²a¡³pyrene¿¡ 72½Ã°£ ³ëÃâ½ÃŲ ÈÄ S. costatum, P. minimum, P. dentatumÀÇ ¼¼Æ÷¼ö´Â 1¡­10 $mu extrm{g}$ L$^{-1}$ÀÇ ³óµµ»çÀÌ¿¡¼­ ±Þ°ÝÇÑ °¨¼Ò¸¦ º¸¿´°í, A. sunguinea, H. akashiwo´Â 0.1¡­1 $mu extrm{g}$ L$^{-1}$ÀÇ ³·Àº ³óµµ¹üÀ§¿¡¼­ Áö¼öÇÔ¼öÀûÀÎ °¨¼Ò¸¦ ³ªÅ¸³Â´Ù. ¼ºÀåÀúÇØ ³óµµ ($IC_{50}$/) ´Â A. sanguinea°¡ 0.10 $mu extrm{g}$ L$^{-1}$·Î °¡Àå ³·¾Ò°í, H. akashiwo (0.74 $mu extrm{g}$ L$^{-1}$) S. costatum (1.68 $mu extrm{g}$ L$^{-1}$), P. dentatum (2.14 $mu extrm{g}$ L$^{-1}$), P. minimum (6.20 $mu extrm{g}$ L$^{-1}$)ÀÇ ¼ø¼­·Î ¼ºÀåÀúÇØ³óµµ°¡ ³ô°Ô ³ªÅ¸³µ´Ù. Benzota¡²a¡³pyrene 1 $mu extrm{g}$ L$^{-1}$ ÀÌÇÏÀÇ ³óµµ¿¡ ³ëÃâµÇ¾ú´ø ¼¼Æ÷µéÀº 5Á¾ ¸ðµÎ ½Ã°£ÀÌ °æ°úÇÔ¿¡ µû¶ó ȸº¹ÇÏ´Â °æÇâÀ» ³ªÅ¸³ÂÀ¸³ª 10 $mu extrm{g}$ L$^{-1}$ÀÌ»óÀÇ ³óµµ¿¡ ³ëÃâ½Ã¿¡´Â P. minimunÀ» Á¦¿ÜÇϰí´Â ȸº¹µÇÁö ¾Ê¾Ò´Ù. ÀÌ·¯ÇÑ °á°úµéÀº Á¶»çµÈ 5Á¾ Áß¿¡¼­ À¯°¢ ¿ÍÆí¸ð·ù P. minimumÀÌ benzo¡²a¡³pyrene¿¡ °¡Àå ³»¼ºÀÌ °­Çϸç, ¹«°¢ ¿ÍÆí¸ð·ù A. sanguinea°¡ °¡Àå ¾àÇÔÀ» ÀǹÌÇÑ´Ù. Benzo¡²a¡³pyrene 0.1, 1, 5, 10 $mu extrm{g}$ L$^{-1}$ ³óµµ¿¡ Heterosigma akashiwo¸¦ ³ëÃâ½ÃŲ ´ÙÀ½ ¼ºÀå¿¡ µû¸¥ ´ÜÀ§¼¼Æ÷ ´ç ±¤ÇÕ¼º·üÀº 0.1, $mu extrm{g}$ L$^{-1}$ÀÇ benzo¡²a¡³pyrene¿¡ ³ëÃâ½ÃŲ ¼¼Æ÷ÀÇ °æ¿ì Áö¼ö¼ºÀå±â¿¡ ´ÜÀ§¼¼Æ÷ ´ç ź¼Òµ¿È­À²(dpm cells$^{-1}$)ÀÌ °¡Àå ³ôÀº ¼öÄ¡¸¦ ³ªÅ¸³»¾ú°í ¾ÈÁ¤±â¿¡ µé¾î°¡ ¸é¼­ Á¡Â÷ °¨¼ÒÇÏ¿© ´ëÁ¶±º°ú À¯»çÇÑ ¾ç»óÀ» ³ªÅ¸³»¾úÀ¸³ª, ÀüüÀûÀ¸·Î ´ëÁ¶±ºº¸´Ù ³ôÀº ¼öÄ¡¸¦ ³ªÅ¸³»¾ú´Ù. ¹Ý¸é¿¡ 5, 10 $mu extrm{g}$ L$^{-1}$ÀÇ ³óµµ¿¡ ³ëÃâµÈ ¼¼Æ÷´Â Ãʱ⿡ ¸Å¿ì ³·Àº ź¼Òµ¿È­À²À» º¸¿´À¸¸ç, 5 $mu extrm{g}$ L$^{-1}$ÀÇ ³óµµ¿¡ ³ëÃâ½ÃŲ ¼¼Æ÷ÀÇ °æ¿ì 12ÀϰºÎÅÍ ´ÜÀ§¼¼Æ÷ ´ç ź¼Òµ¿È­À²ÀÌ ¸Å¿ì Å©°Ô Áõ°¡ÇÏ¿´´Ù. º» ¿¬±¸ÀÇ °á°úµéÀº ¿¬¾ÈÇØ¿ª¿¡ benzo¡²a¡³pyrene°ú °°Àº Áö¼Ó¼º À¯±â¿À¿°¹°ÁúÀÌ À¯ÀԵǾúÀ» ¶§ ³»¼º¿©ºÎ¿¡ µû¶ó ½Ä¹°Çöûũſ ±ºÁý³» Á¾ õÀÌ¿Í ÀÏÂ÷»ý»ê·Â¿¡ Å©°Ô ¿µÇâÀ» ¹ÌÄ¥ ¼ö ÀÖÀ½À» ½Ã»çÇÑ´Ù.
We examined the impacts of anthyopogenic pollutant (benzo¡²a¡³pyrene) on the growth and photosynthesis of five marine phytoplankton species (Skeletonema costatum, Heterosigma akashiwo, Prorocentrum dentatum, P. minimum, Aknshiwo sanguinea), which are dominant in Korean coastal water. After the 72 h exposure to benzo¡²a¡³pyrene, the dramatic decrease in cell numbers was observed in the range of 1 to 10 $mu extrm{g}$ L$^{-1}$ for S. costatum, P. minimum, P. dentatum, whereas for A. sanguinea and H. akashiwo at the low concentrations 0.1 to 1 $mu extrm{g}$ L$^{-1}$ . Among the 5 phytoplankton species, the highest growth inhibition concentration ($IC_{50}$/) was 6.20 $mu extrm{g}$ L$^{-1}$ for P. minimum, followed by 2.14 $mu extrm{g}$ L$^{-1}$ for P. dentatum, 1.68 $mu extrm{g}$ L$^{-1}$ for S. costatum, 0.74 $mu extrm{g}$ L$^{-1}$ for H. akashiwo, 0.10 $mu extrm{g}$ L$^{-1}$ for A. sanguinea. The five species exposed to the low concentration of 1 $mu extrm{g}$ L$^{-1}$ were recovered after transferring to new media, but the species exposed to the high concentrations of 10 and 100 $mu extrm{g}$ L$^{-1}$ were not recovered, with the exception of P. minimum. Those results indicate that the thecate dinoflagellate P. minimum is most tolerant to the chemical and the athecate dinoflagellate A. sanguinea is not. Geneyally, the cell-specific photosynthetic capacity of H. akashiwo exposed to the low concentrations of 0.1 and 1 $mu extrm{g}$ L$^{-1}$ was higher than that of the cells in the control, whereas the cells exposed to the high concentrations of 5 and 10 $mu extrm{g}$ L$^{-1}$ showed the negligible photosynthetic level by the first few days of the experiment. In the case of the cells exposed to the concentration of 5 $mu extrm{g}$ L$^{-1}$ , after 12 days of the experiment the photosynthetic capacity was increased toward the end of the experiment. This indicates that H. akashiwo may utilize the benzo¡²a¡³pyrene as a carton source for its growth when exposed to low concentrations. Results suggest that anthropogenic pollutants such as benzo¡²a¡³pyrene may have significant influence on the succession of phytoplankton species composition and the primary production in coastal marine environments.
 
Ű¿öµå
benzopyrene;72 h$IC_{50}$;growth inhibition;photosynthesis;phytoplankton;PAHs;
 
ȯ°æ»ý¹° / v.22, no., 2004³â, pp.54-62
Çѱ¹È¯°æ»ý¹°ÇÐȸ
ISSN : 1226-9999
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200411922292889)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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