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Çѱ¹È¯°æ»ý¹°ÇÐȸ / v.22, no.1, 2004³â, pp.148-152
¹éµÎ»ê ÀÚ»ý Âüµ¹²ÉÀÇ »ýÀ°°ú ÀÌ¿ë I. ¹è¾ç¼¼Æ÷ À¯µµ¿Í À¯¸®¾Æ¹Ì ³ë»ê Á¶¼º
( Growth and Utility of Rhodiola sachalinensis in Baekdu Mountain I. Induction of Callus and Composition of Free Amino Acids )
¿ÀÀμ÷;¼Ò»ó¼·;Çã¸íÀÚ; ÀüºÏ´ëÇб³ ÀÚ¿¬°úÇдëÇÐ »ý¹°°úÇкÎ;ÀüºÏ´ëÇб³ ÀÚ¿¬°úÇдëÇÐ »ý¹°°úÇкÎ;¿¬º¯´ëÇÐ ³óÇпø ³óÇаè;
 
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º» ½ÇÇèÀº ¹éµÎ»ê ÀÚ»ý Âüµ¹²ÉÀÇ ¹ß¾ÆÁ¶°Ç°ú Á¾ÀÚ, À¯½Ä¹° ¹× »Ñ¸®À¯·¡ Ķ·¯½ºÀÇ À¯¸® ¾Æ¹Ì³ë»ê Á¶¼º°ú ÇÔ·®À» Á¶»çÇÏ¿© ´ÙÀ½°ú °°Àº °á°ú¸¦ ¾ò¾ú´Ù. »Ñ¸®·ÎºÎÅÍ Ä¶·¯½º À¯µµ´Â 2,4-D ¹× NAA°¡ ÷°¡µÈ MS¹èÁö¿¡¼­ À¯µµµÇ¾ú´Ù. Ķ·¯½º Çü¼ºÀ²Àº 2,4-D 2.0 mg $L^{-1}$¿¡ kinetin 1.0 mg $L^{-1}$ È¥¿ë 󸮱¸°¡ NAA 2.0 mg $L^{-1}$¿¡ kinetin 1.0 mg $L^{-1}$ È¥¿ë 󸮱¸º¸´Ù Ķ·¯½º Çü¼ºÀ²ÀÌ ³ô¾Ò´Ù. À¯¸® ¾Æ¹Ì³ë»êÀº Á¾ÀÚ, ÁÙ±â, »Ñ¸® ±×¸®°í Ķ·¯½º¿¡¼­ aspartic acid, glutamic acid, serine ¹× glycine µîÀÇ 25¡­26Á¾ÀÌ °ËÃâµÇ¾úÀ¸¸ç Á¾ÀÚ, Áٱ⠱׸®°í »Ñ¸®ºÎÀ§¿¡¼­´Â lysineÀÌ °¡Àå ¸¹Àº ¾çÀ» ³ªÅ¸³ÂÀ¸¸ç Ķ·¯½º¿¡¼­´Â histidineÀÌ ³ôÀº ¾çÀ» ³ªÅ¸³Â´Ù. ÀüüÀûÀÎ À¯¸®¾Æ¹Ì³ë»ê ºÐÆ÷´Â lysine, histidine ¹× arginineÀÇ ÇÔ·®ÀÌ ³ô¾Æ Âüµ¹²É À¯½Ä¹° ¹× Ķ·¯½ºÀÇ °æ¿ì ¿°±â¼º ¾Æ¹Ì³ë»êÀÇ ºÐÆ÷°¡ ³ôÀº ½Ä¹°ÀÓÀ» ¾Ë ¼ö ÀÖ¾ú´Ù. ¶ÇÇÑ ÃÑ À¯¸® ¾Æ¹Ì³ë»ê ÇÔ·®Àº Á¾ÀÚ¿Í »Ñ¸®, Áٱ⿡ ºñÇØ Ķ·¯½º¿¡¼­ ½Å¼±Áß g´ç 500¡­l,000$mu$mo1eÁ¤µµ ³ô¾Æ ¿ì¼öÇÑ ¾à¸®ÀÛ¿ëÀ» Áö´Ï°í ÀÖ´Â Âüµ¹²ÉÀÇ ¼ººÐÀ» ¹è¾ç¼¼Æ÷¸¦ ÅëÇÏ¿© ´ë·®»ý»êÀÌ °¡´ÉÇÔÀ» ½Ã»çÇϰí ÀÖ´Ù.
This study was carried out to investigate the callus formation and to determine the composition and contents of the Hee amino acids in seedlings and callus of Rhodiola sachalinensis. The callus formation from the part of seedling-roots was the most effective on MS (Murashige and Skoog) media supplemented with 2.0 mg $L^{-1}$2,, 4-D included in 1.0 mg $L^{-1}$ kinetin than the other experimental plots which prepared with the different concentration of the various growth regulators. Free amino acids extracted from the explants and the callus were a total of 25¡­26 kinds. Especially, the basic amino acids such as arginine, lysine and histidine released in callus were found relatively in a large quantity. These results suggest that the cultured callus of Rhodiola sachatinensis could induce for the mass production of the other useful ingredients.
 
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Rhodiola sachalinensis;free amino acids;callus;
 
ȯ°æ»ý¹° / v.22, no.1, 2004³â, pp.148-152
Çѱ¹È¯°æ»ý¹°ÇÐȸ
ISSN : 1226-9999
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200411922293216)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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