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Çѱ¹È¯°æ»ý¹°ÇÐȸ / v.22, no.3, 2004³â, pp.394-399
Serratia marcescens LSY4 Áß±Ý¼Ó ³»¼ºÁÖ¸¦ ÀÌ¿ëÇÑ BOD¼¾¼­ÀÇ °¨ÀÀµµ
( Sensitivity of BOD Sensor with Heavy Metal Tolerant Serratia marcescens LSY4 )
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S. marcescens LSY4°¡ ÃæÀüµÈ BOD¼¾¼­¸¦ Á¦ÀÛÇÏ¿© Ç¥ÁØ¿À¿°¹°ÁúÀ» Æ÷ÇÔÇÑ ¼ö¿ë¾×ÀÇ BOD¸¦ ÃøÁ¤ÇÏ¿´´Ù. ¹è¾ç½Ã°£ÀÌ 9¡­16½Ã°£ÀÏ ¶§¿¡´Â ¹è¾ç½Ã°£¿¡ µû¸¥ BOD¼¾¼­ÀÇ °¨ÀÀµµ¿¡ Å« Â÷À̸¦ ³ªÅ¸³»Áö ¾Ê¾ÒÀ¸¸ç, ±Õü·®µµ 0.22~0.75mg $cm^{-2}$ÀÇ ¹üÀ§¿¡¼­´Â °ÅÀÇ µ¿ÀÏÇÑ ¼¾¼­ÀÇ °¨ÀÀµµ¸¦ ³ªÅ¸³»¾ú´Ù. ¼ö¿ë¾×ÀÇ pH°¡ 4~9»çÀÌ·Î º¯È­ÇÒ °æ¿ì ¼¾¼­ÀÇ °¨ÀÀµµ°¡ °¡¿ªÀûÀ¸·Î º¯È­ÇÏ¿´À¸³ª ¼ö¿ë¾×ÀÌ ´õ »ê¼ºÀ̰ųª ´õ ¿°±â¼ºÀÌ µÇ¸é ¼¾¼­ÀÇ °¨ÀÀµµ°¡ ºñ°¡¿ªÀûÀ¸·Î ÀúÇϵǾú´Ù. ¼ö¿ë¾×¿¡ Á߱ݼÓÀÌ¿ÂÀÌ Ã·°¡µÇ¸é ¼¾¼­ÀÇ °¨ÀÀµµ°¡ °¨¼ÒÇÏ¿´À¸¸ç, $Zn^{2+}$³ª $Cd^{2+}$º¸´Ù $Cu^{2+}$ȤÀº $Ag^+$°¡ ÷°¡µÇ¾úÀ» ¶§ ¼¾¼­ÀÇ °¨ÀÀµµ°¡ ´õ ±Þ°ÝÈ÷ °¨¼ÒÇÏ¿´´Ù. Á߱ݼӿ¡ ´ëÇÑ ³»¼ºÀÌ À¯µµµÈ ±Õü¸¦ ÃæÀüÇÏ¿´À» ¶§ Á߱ݼÓÀÌ¿ÂÀÇ Ã·°¡¿¡ µû¸¥ ¼¾¼­ÀÇ °¨ÀÀµµ °¨¼Ò°¡ Å©°Ô ¿ÏÈ­µÇ¾úÀ¸¸ç, ÀÌ·± È¿°ú´Â $Cd^{2+}$³»¼ºÁÖº¸´Ù $Cu^{2+}$³»¼ºÁÖ¿¡¼­ ´õ ÇöÀúÇÏ¿´´Ù.
A BOD sensor was prepared with S. marcescens LSY4 and was applied for measurement of BOD values of a solution containing the standard organic pollutants. The sensor sensitivity was nearly independent of the culture time in the range of 9-16 hours. It was also affected little by the cell mass in the range of 0.22-0.75 mg $cm^{-2}$. A cyclic change in the solution pH in the range of 4-9 was accompanied by a reversible variation in the sensor sensitivity. However, the reversibility was lost when the solution pH became more acidic or more basic. Heavy metal ions lowered the sensor sensitivity, which took place more precipitously in the presence of $Cu^{2+}$ and $Ag^+$ rather than in the presence of $Zn^{2+}$ and $Cd^{2+}$. The reduction of the sensor sensitivity was significantly attenuated by loading heavy metal ion tolerance induced strain. The $Cu^{2+}$tolerance induced strain was more efficient for the attenuation than $Zn^{2+}$ and $Cd^{2+}$ tolerance induced strain.
 
Ű¿öµå
Microbial BOD sensor;heavy metal ion tolerance;Serratia marcescens LSY4;
 
ȯ°æ»ý¹° / v.22, no.3, 2004³â, pp.394-399
Çѱ¹È¯°æ»ý¹°ÇÐȸ
ISSN : 1226-9999
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200412910473770)
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