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한국환경생물학회 / v.23, no.1, 2005년, pp.21-26 
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MCF-7 세포주의 γ선에 의한 DNA 손상 반응 유전자 발현 양상의 분석
( A DNA-Damage Response Gene Expression Analysis in MCF-7 followed by γ-Radiation ) |
| 박지윤;황창일;박웅양;김진규;채영규; 한양대학교 생화학 및 분자생물학과;서울대학교 의과대학 생화학교실;서울대학교 의과대학 생화학교실;한국원자력연구소 동위원소 방사선 응용팀;한양대학교 생화학 및 분자생물학과;
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| 초 록 |
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| Cell response to genotoxic agents is complex and involves the participation of different classes of genes including cell cycle control, DNA repair and apoptosis. In this report, we presented a approach to characterize the cellular functions associated with the altered transcript profiles of MCF-7 exposed to low-dose in vitro gamma-irradiation. We used the method of human 2.4 k cDNA microarrays containing apoptosis, cell cycle, chromatin, repair, stress and chromosome genes to analyze the differential gene expression characterization that were displayed by radiation-exposed cell, human breast carcinoma MCF-7 cell line, such as 4 Gy 4 hr, 8 Gy 4 hr, and 8 Gy 12 hr. Among these genes, 66 were up-regulated and 49 were down-regulated. Specific genes were concomitantly induced in the results. Cyclin dependent kinase 4 (Cdk4) is induced for starting the cell cycle. This regulation is required for a DNA damageinduced G1 arrest. In addition to, an apoptotic pathways gene Bcl-w was concomitantly induced. Mismatch repair protein homologue-l (hMLH1), a necessary component of DNA mismatch protein repair (MMR), in G2-M cell cycle checkpoint arrest. The present study provides new information on the molecular mechanism underlying the cell response to genotoxic stress, with relevance to basic and clinical research. |
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| 키워드 |
| irradiation;MCF-7;DNA-damage response;gene expression; |
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환경생물 / v.23, no.1, 2005년, pp.21-26
한국환경생물학회
ISSN : 1226-9999
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200516610524612)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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