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한국환경생물학회 / v.23, no.3, 2005년, pp.269-274 
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( Genetic Engineering for Detection of Endocrine Disruption using I-18 C Gene Expression in Chironomus riparius ) |
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| 초 록 |
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| The 2D/E gel analysis for polypeptide expression reflecting I-18 C gene (early-ecdysterone inducible gene) has conducted the emerged C. riparius adults from larval phase exposure to tebufenozide acting as an ecdysteroidal molting hormone. Control group, the amount of ORE II of the I-18 C gene was larger than that of ORE I of this gene. After treatments, ORE I of the I-18 C gene was overexpressed as the polypeptide, whereas ORF II of this gene was expressed as the polypeptide and was clearly reduced expression. Accordingly, we consider that tebufenozide exhibited endocrine disruptions related processing of ecdysteroid receptor protein reflecting ORF II of I-18 C gene. Also, earlier emergence day was related overexpressed polypeptide reflecting ORE I of I-18 C gene. In this study result, tebufenozide induced changing of physiological condition, and then polypeptide expression reflecting early-ecdysterone inducible I-18 C gene was different between control group and exposure group. |
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| 키워드 |
| Chironomus riparius;I-l8C gene;polypeptide expression;tebufenozide; |
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환경생물 / v.23, no.3, 2005년, pp.269-274
한국환경생물학회
ISSN : 1226-9999
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200504840632335)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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