|
|
Çѱ¹È¯°æ»ý¹°ÇÐȸ / v.24, no.1, 2006³â, pp.38-45
|
¼öÀº, 6°¡Å©·Ò ¿À¿°Åä¾çÀ¸·ÎºÎÅÍ ºÐ¸®µÈ ¹æ¼±±Õ±ºÀÇ »ýÅÂÇÐÀû Ư¼º
( Ecological Characteristics of Actinomycetes from Mercury and Chrome Polluted Soil ) |
Á¶¹ÎÇý;ÇÑ»ó¹Ì;¹éÇÏÁÖ;Ȳ°æ¼÷; ¸ñ¿ø´ëÇб³ ¹ÙÀÌ¿À»ê¾÷Çаú;³óÃÌÁøÈïû ³ó¾÷°úÇбâ¼ú¿ø ³ó¾÷»ý¹°ºÎ;°æ»óºÏµµ º¸°Çȯ°æ¿¬±¸¿ø;¸ñ¿ø´ëÇб³ ¹ÙÀÌ¿À»ê¾÷Çаú;
|
|
|
 |
|
|
ÃÊ ·Ï |
°æ»óºÏµµ³»ÀÇ ±Ý¼Ó±¤»ê, °øÀå ¹× °ø¾÷Áö¿ªÀÇ Åä¾çÀÇ ¿À¿°µµ¸¦ Á¶»çÇϰí Áß±Ý¼Ó ¿À¿°Åä¾ç Áß¿¡ ºÐÆ÷ÇÏ´Â ¹Ì»ý¹° floraÀÇ Á¤·®Àû Æò°¡¸¦ À§ÇÏ¿© ûÁ¤Áö¿ªÀÎ µ¶µµÅä¾ç°ú ºñ±³ÇÑ °á°ú, ÀϹݼ¼±ÕÀÇ °æ¿ì $10sim100$¹è ³·Àº ºÐÆ÷À²À» ³ªÅ¸³»¾úÀ¸¸ç, 6°¡Å©·Ò ¿À¿°Åä¾ç Áß °õÆÎÀÌ´Â °ÅÀÇ °ËÃâµÇÁö ¸¹¾Ò´Ù. ¹æ¼±±ÕÀº ¼öÀº ¿À¿°Åä¾çÀÇ °æ¿ì $6.7X10^5sim7.5X10^7;cfu;g^{-1}$, 6°¡Å©·Ò ¿À¿°Åä¾ç ³» ¹æ¼±±Õ ¼ö´Â $4.8X10^4sim5.0X10^5;cfu;g^{-1}$ÀÌ ÃøÁ¤µÇ¾î ´ëÁ¶±¸ Åä¾ç ³» ¹æ¼±±Õ ºÐÆ÷¿Í ºñ½ÁÇÑ ³ôÀº ºÐÆ÷À²À» ³ªÅ¸³»¾ú´Ù. ¼öÀº ¿À¿°Åä¾ç ½Ã·á¸¦ $HgCl_2$ 50ppm°ú 100ppmÀ» °¢°¢ ÷°¡ÇÑ ¹èÁö¿¡ Á¢Á¾ÇÏ¿© ¹æ¼±±Õ¼ö¸¦ ÃøÁ¤ÇÑ °á°ú ´ëÁ¶±¸ Åä¾ç¿¡ ºñÇØ 10¹è ÀÌ»óÀÇ ³ôÀº ¼ö¸¦ È®ÀÎÇÏ¿´´Ù. ¼öÀº ¹× 6°¡Å©·ÒÀÇ ¿À¿°Åä¾çÀ¸·ÎºÎÅÍ ºÐ¸®µÈ ¹æ¼±±Õ 150 ±ÕÁÖ¿¡ ´ëÇØ Ç×±Õ·ÂÀ» °ËÅäÇÑ °á°ú 31±ÕÁÖ°¡ ¾ç¼º¹ÝÀÀÀ» ³ªÅ¸³»¾ú´Ù. ÀÌµé ¹æ¼±±Õ Áß °ÇÑ Ç×±ÕȰ¼ºÀ» ³ªÅ¸³¾ 10±ÕÁÖ¸¦ ¼±¹ßÇÏ¿© °èÅëÇÐÀû Ư¼ºÀ» °ËÅäÇÑ °á°ú Streptomyces ¼Ó, Saccharopolyspora ¼Ó, ±×¸®°í Nocardioides ¼Ó¿¡ ¼ÓÇÏ´Â 3°³ÀÇ °èÅ뱺À¸·Î ºÐ·ùµÇ¾ú´Ù. ÀÌµé ºÐ¸®±ÕÁÖÁß¿¡´Â ±âÁö ¹Ì»ý¹°Á¾°ú 97% ÀÌÇÏÀÇ ³·Àº »óµ¿¼ºÀ» ³ªÅ¸³»´Â ½Å±Ô¹Ì»ý¹°µµ ´Ù¼ö Æ÷ÇԵǾî ÀÖ¾ú´Ù. ÀÌ»óÀÇ °á°ú·ÎºÎÅÍ, º» ¿¬±¸¿¡¼ Á¶»çµÈ ¼öÀº ¹× 6°¡Å©·Ò ¿À¿°Åä¾ç³» Áß±Ý¼Ó ³»¼º ¹× Ç×±ÕȰ¼ºÀ» °®´Â ¹æ¼±±ÕÀÌ ³ô°Ô ºÐÆ÷ÇØ ÀÖÀ½ÀÌ È®ÀεǾî, ³ºÐÇØ¼º ÈÇÕ¹°À» Æ÷ÇÔÇÑ ´Ù¾çÇÑ ±¸Á¶ÀÇ À¯ÇØ µ¶¼º¹°ÁúÀÇ Á¤±ÕÀÛ¿ëÀ¸·Î ÀÎÇÑ Áß±Ý¼Ó ¿À¿°Åä¾ç ³» ȯ°æÀÇ º¹¿øÀÌ È¿À²ÀûÀ¸·Î ÀÌ·ç¾î Áú ¼ö ÀÖÀ» °ÍÀ¸·Î ±â´ëµÇ¾ú´Ù.>¸¦ »ý»êÇÏ´Â °ÍÀ» È®ÀÎÇÏ¿´´Ù., ±× ´ÙÀ½ÀÌ ¸¶´ëÆ÷Àå ³ÃÀåÀúÀå, Ç×±ÕÆ÷Àå ½Ç¿ÂÀúÀå, ¸¶´ëÆ÷Àå ½Ç¿ÂÀúÀå ¼øÀ¸·Î ÇâÃë°¡ °¨¼ÒÇÏ¿´´Ù. ÀÌ´Â Æ÷ÀåÀçÀÇ Â÷À̺¸´Ù´Â Àú¿Âº¸°ü ¿©ºÎ°¡ ÁÖ¿ä º¯ÀÎÀ¸·Î ÀÛ¿ëÇÑ´Ù°í ÇÒ ¼ö ÀÖ´Ù. °á·ÐÀûÀ¸·Î ´õ´ö °íÀ¯ÀÇ ÇâÃë¿¡ ´ëÇÑ °ü´ÉÆò°¡¸¦ ÅëÇØ ÀúÀå´õ´öÀÇ »óǰ°¡Ä¡¸¦ ÆÇ´ÜÇÒ ¼ö ÀÖ´Â ±âÁØÀÌ ¸¶·ÃµÉ ¼ö ÀÖÀ» °ÍÀ¸·Î ±â´ëµÈ´Ù.1%·Î »ó´ç¼ö¿¡¼ °ñ»óŰ¡ ºñÁ¤»óÀÓÀ» ¾Ë ¼ö ÀÖ¾ú´Ù. º» ¿¬±¸¿¡¼´Â ½ºÅ×·ÎÀÌµå »ç¿ë±â°£ ¹× ÃàÀû¿ë·®ÀÌ °ñ»ó³»ÀÇ º¯È¿¡ Ưº°ÇÑ ¿µÇâÀ» ¹ÌÄ¡Áö ¾Ê´Â °ÍÀ¸·Î ³ªÅ¸³µ´Ù. µ¿ÀÏ È¯ÀÚµéÀÇ °ñ»óÅÂÀÇ º¯È°üÂû°ú ½ÅÁúȯ °ü·Ã °ñ°¨¼ÒÀÇ ¿äÀÎÀ» ¹àÇô³»±â À§ÇÑ Ãß°¡ÀûÀÎ ¿¬±¸°¡ ÇÊ¿äÇÒ °ÍÀ¸·Î »ç·áµÈ´Ù. Á¤È®ÇÑ Áø´Ü ¹× µ¿¹ÝµÈ ÁúȯÀ» °¨º°Çϱâ À§ÇÑ ³ë·ÂÀÌ ÇÊ¿äÇÏ´Ù.½ÉµÇ³ª X-ray VCUG·Î ¹ß°ßµÇÁö ¾ÊÀº °æ¿ì¿¡´Â RI VCUG¸¦ ²À ½ÃÇàÇÏ´Â °ÍÀÌ ¹æ±¤¿ä°ü¿ª·ùÀÇ Á¤È®ÇÑ Áø´ÜÀ» Çϴµ¥ µµ¿òÀÌ µÈ´Ù..25% sodium ½ÄÀÌ enalapril±º¿¡¼ »ç±¸Ã¼¿©°úÀ²ÀÌ Áõ°¡µÊÀ» °üÂûÇÒ ¼ö ÀÖ¾ú´Ù. 4) ½ÅÀýÁ¦¼úÈÄ ³²¾Æ ÀÖ´Â ½ÅÁ¶Á÷¹«°Ô¸¦ ºñ±³ÇÏ¿© º¸¸é 24ÁÖ° 0.25% sodium ½ÄÀ̱º, 0.25% sodium ½ÄÀÌ enalapril±º, 0.25% sodium ½ÄÀÌ nicardipine±º¿¡¼ 16ÁÖ° 0.49% sodium ½ÄÀ̱º, 0.49% sodium ½ÄÀÌ enalapril±º, 0.49% sodium ½ÄÀÌ nicardipine ±ºº¸´Ù ÀÇÀÇÀÖ°Ô ½ÅÁ¶Á÷¹«°Ô°¡ Áõ°¡µÊÀ» °üÂûÇÒ ¼ö ¾ø¾ú´Ù. 5) 0.25% sodium ½ÄÀ̱ºÀº 0.49% sodium ½ÄÀ̱º°ú ºñ±³ÇÏ¿© MESÀÇ ÇöÀúÇÑ °¨¼Ò¸¦ º¸¿´°í (0.25% sodium½ÄÀ̱º: 12ÁÖ; $1.97{pm}0.02$, 24ÁÖ; $2.06{pm}0.03$ vs. 0.49% sodiu |
|
Ecological characteristics of microbial populations inhabiting heavy metal polluted soil were investigated. The samples were collected from 293 sites around an factory and industry at Gyeoungsangbuk-do. We measured the contents of seven heavy metal elements (Cd, Cu, As, Hg, Pb, $Cr^{6+}$, CN), seven sites have been seriously contaminated by mercury and chrome. A quantitative evaluation of microbial populations in mercury and chrome contaminated soil was examined by using plate count method. Bacterial numbers in polluted soil samples ranged from $7.4X10^5;to;9.3X10^7;cfu;g^{-1}$, about $10sim100$ fold less than the count for the unpolluted soil. Moulds were not detected in chrome polluted soil. The log values of actinomycetes of each contaminated soil samples were log ranged from 6.18 to 7.52. The ratio of actinomycetes was similar to unpolluted soil. The investigation showed actinomycetes to be the major microbial population inhabiting the mercury and chrome polluted soil. Thirty-one isolates among the total isolates were examined for antibacterial activity. These isolates were identified based on a phylogenetic analysis using 16S rRNA gene nucleotide sequences, they were categorized in three major phylogenetic groups, belong to the Streptomyces (6 strains), Saccharopolyspora (3 strains), Nocardiodes (1 strain). On the phylogenetic tree, the clade consisting of five isolates were distantly related to all of the established Streptomycetes genera, indicating the possibility as members of new species. |
|
Ű¿öµå |
Actinomycetes;Chrome;Mercury;Micrflora phylogeng; |
|
|
|
 |
|
ȯ°æ»ý¹° / v.24, no.1, 2006³â, pp.38-45
Çѱ¹È¯°æ»ý¹°ÇÐȸ
ISSN : 1226-9999
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200617033417278)
¾ð¾î : Çѱ¹¾î |
|
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø |
|
|
|
|
|