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Çѱ¹È¯°æ»ý¹°ÇÐȸ / v.28, no.4, 2010³â, pp.223-230
Á¦ÁÖ ¿¬¾È¿¡¼­ ºÐ¸®µÈ ÇØ¾ç¹æ¼±±ÕÀÇ ÀÌÈ­ÇÐÀû Ư¼º ¹× ´Ù¾ç¼º
( Physico-chemical Characteristics and Diversity of Marine Actinomycetes Isolated from the Coast of Jeju Island )
±è¸¸Ã¶;Çã¹®¼ö; Á¦ÁÖ´ëÇб³ ¼ö»ê»ý¸íÀÇÇаú;Á¦ÁÖ´ëÇб³ ¼ö»ê»ý¸íÀÇÇаú;
 
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Á¦ÁÖµµ ¿¬¾ÈÇØ¿ª 4°³ Áö¿ª(ÇѸ², ¾Ö¿ù, ½ÅÃÌ, ÇÔ´ö) ÇØ¼öÀÇ ¿Âµµ, ¿°ºÐ³óµµ, ¿ëÁ¸»ê¼Ò·®(DO), È­ÇÐÀû »ê¼Ò¿ä±¸·®(COD), ºÎÀ¯¹°Áú(SS), ¾Ï¸ð´Ï¾Æ¼º Áú¼Ò($NH_3-N$), Áú»ê¼º Áú¼Ò($NO_3-N$) ¹× ¾ÆÁú»ê¼º Áú¼Ò($NO_2-N$)¿Í °°ÀÌ ´Ù¾çÇÑ ÀÌÈ­ÇÐÀû Ư¼ºÀ» È®ÀÎÇÏ¿´´Ù. ÇØ¼öÀÇ Æò±Õ ¿Âµµ´Â $26.23{sim}28.6^{circ}C$, ¿°ºÐ³óµµ´Â 31.4~32.88¢¶, pH´Â 8.15~8.35, COD´Â 0.48~0.91 mg $L^{-1}$, DO´Â 6.78~6.87 mg $L^{-1}$·Î ³ªÅ¸³µ´Ù. ÇØ¼ö¿¡¼­ ºÐ¸®µÈ ÇØ¾ç¹æ¼±±ÕÀº ÃÑ 52Á¾À¸·Î Á¦Áֽà µ¿ºÎÁö¿ª(A, B)¿¡¼­´Â 24±ÕÁÖ, ¼­ºÎÁö¿ª(C, D)Àº 28±ÕÁÖ°¡ ºÐ¸®µÇ¾ú´Ù. ºÐ¸®µÈ ÇØ¾ç¹æ¼±±ÕÀº 16S rRNA ¿°±â¼­¿­ ºÐ¼®À» ÀÌ¿ëÇÏ¿© ÃÖÁ¾ÀûÀ¸·Î µ¿Á¤µÇ¾úÀ¸¸ç, ¿°±â¼­¿­ Á¤º¸¸¦ ±âÃÊ·ÎÇÏ¿© À¯»çµµ(similarity)¸¦ Á¶»çÇÏ¿´´Ù. ºÐ¸®µÈ ¹æ¼±±ÕÀÇ 16S rRNA ¿°±â¼­¿­ ºÐ¼®À» ÅëÇÏ¿© °èÅë ºÐ·ùÇÐÀûÀ¸·Î ¾î¶² division¿¡ ¼ÓÇÏ´ÂÁö¸¦ È®ÀÎÇÏ¿© º» °á°ú Á¦ÁÖµµ Á¦Áֽà µ¿ºÎÁö¿ª(Site A, B) ÇØ¾ç¿¡¼­ ºÐ¸®µÈ ¹æ¼±±Õ 24±ÕÁÖ´Â Gram positive bacteria (division)/Actinobacteria (class)/Actinomycetales (order)/Streptomy-cineae (suborder)/Streptomycataceae (family)/Streptomyces(genus)¿¡ 22 ±ÕÁÖ, Actinomycetales (order)/Streptosporangineae (suborder)/Nocardiopsaceae (family)/Nocardiopsis (genus)¿¡ 2 ±ÕÁÖ°¡ ºÐ¸®µÇ¾ú´Ù. Á¦Áֽà ¼­ºÎÁö¿ª(Site C, D) ÇØ¾ç¿¡¼­ ºÐ¸®µÈ ¹æ¼±±Õ 28±ÕÁÖ´Â Gram positive bacteria (division)/Actinobacteria (class)/Actinomycetales (order)/Streptomycineae (suborder)/Streptomycataceae (family)/Streptomyces (genus)¿¡ 27±ÕÁÖ, Actinomycetales (order)/Streptosporangineae (suborder)/Nocardiopsaceae (family)/Nocardiopsis (genus)¿¡ 1±ÕÁÖ°¡ ºÐ¸®µÇ¾ú´Ù.
To investigate the variations of physico-chemical factors in four stations (Hanlim, Aewol, Sinchon, Hamdeok) at Jeju coastal area, Water temperature, Salinity, dissolved oxygen (DO), pH, chemical oxygen demand (COD), suspended solid (SS), ammonia-nitrogen ($NH_4-N$), nitrite-nitrogen ($NO_3-N$), nitrate-nitrogen ($NO_2-N$) were analysed. The ranges of water temperature were from 26.23 to $28.6^{circ}C$, the salinity were from 31.4 to 32.88¢¶, the pH were from 8.15 to 8.35, the chemical oxygen were from 0.48 to 0.91 mg $L^{-1}$. A total of 52 strains of marine actinomycetes was isolated from Jeju Island coastal area. They were characterized by determining morphological and physio-biochemical properties, the API kit and confirmed by molecular methods including partial sequencing of 16S rRNA. A neighbor-joining tree of partial 16S rRNA sequences divided the 52 isolates in 2 major groups, 22 strains of Gram positive bacteria/Actinobacteria (division)/Actinomycetales (order)/Streptomycineae (suborder)/Streptomycetaceae (family)/Streptomyces (93.1%) and 2 strains of Streptospotangineae (suborder)/Nocardiopsaceae (family)/Nocardiopsis (6.9%).
 
Ű¿öµå
physico-chemical;marine actinomycetes;Jeju;16S rRNA;
 
ȯ°æ»ý¹° / v.28, no.4, 2010³â, pp.223-230
Çѱ¹È¯°æ»ý¹°ÇÐȸ
ISSN : 1226-9999
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO201015441599402)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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