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Çѱ¹È¯°æ»ýÅÂÇÐȸ / v.17, no.2, 2003³â, pp.169-176
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RAPD¸¦ ÀÌ¿ëÇÑ ÀÚ»ý ¹Îµé·¹ Á¾°ú ±ÍÈ ¹Îµé·¹ Á¾°£ÀÇ ¿¬°ü°è ºÐ¼®
( Analysis of Genetic Relationship Among Native Taraxacum and Naturalized Taraxacum species using RAPD ) |
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RAPD¸¦ ÀÌ¿ëÇÏ¿© ±¹³»¿¡ »ýÀ°ÇÏ´Â 4Á¾ÀÇ ÀÚ»ý ¹Îµé·¹¿Í 2Á¾ÀÇ ±ÍÈ ¹Îµé·¹ Á¾°£ÀÇ À¯ÀüÀûÀÎ À¯¿¬°ü°è¸¦ ºÐ¼®ÇÏ¿´´Ù Primer ScreeningÀ» ÅëÇØ ¼±¹ßµÈ 30°³ÀÇ Primer¸¦ ÀÌ¿ëÇÏ¿© RAPD¸¦ ÇàÇÑ °á°ú, Polymorphic band ¼ö´Â 141°³·Î ³ªÅ¸³ª ¹Îµé·¹ Á¾°£ÀÇ À¯ÀüÀûÀÎ Â÷ÀÌ ºÐ¼®ÀÌ °¡´ÉÇÏ¿´´Ù. Primer OPC12, OPD16, OPK16, OPK17, OPK20, OPSI¿¡¼´Â °¢ Á¾¸¶´Ù. ƯÁ¤ ¹êµå°¡ ÀÖ´Â °ÍÀ¸·Î Á¶»çµÇ¾ú´Ù. ƯÈ÷ OPS8¿¡¼´Â 564bp¿¡¼ ±ÍÈÁ¾ÀÎ ¼¾ç¹Îµé·¹ Á¾¿¡¼¸¸ ƯÀÌ ¹êµå°¡ ³ªÅ¸³µ´Ù. RAPDºÐ¼®°á°ú ÀÚ»ýÁ¾°ú ±ÍÈÁ¾ ¹Îµé·¹µéÀº ¸íÈ®È÷ 2±ºÀ¸·Î ±¸ºÐµÇ¾ú´Ù. 1±º¿¡´Â ¼¾ç¹Îµé·¹. ºÓÀº¾¾¼¾ç¹Îµé·¹ µîÀÇ ±ÍÈÁ¾ ±×·ìÀ̾úÀ¸¸ç, II±ºÀº ¹Îµé·¹, »ê¹Îµé·¹, Á»¹Îµé·¹, Ⱥ¹Îµé·¹ µîÀÇ ÀÚ»ýÁ¾ ¹Îµé·¹ ±×·ìÀ¸·Î ³ª´µ¾îÁ³´Ù. Bootstrap ¹æ¹ýÀ¸·Î 6Á¾ÀÇ À¯¿¬°ü°è¸¦ ºÐ¼®ÇÑ °á°úµµ ºñÀ¯»ç°è¼ö¸¦ ÅëÇÑ ¹æ¹ýÀ¸·Î ºÐ¼®ÇÑ °á°ú¿Í ¸Å¿ì À¯»çÇÏ¿´´Ù ¿ì¸® ³ª¶ó¿¡¼ ÀÚ»ýÇÏ´Â ¹Îµé·¹¼Ó 6Á¾ÀÇ ÁÖ¿äÇüÁúÀ» Á¶»çÇÑ °á°ú I±º¿¡ ¼ÓÇÏ´Â ¹Îµé·¹·ù´Â II±º¿¡ ¼ÓÇÑ Á¾µé¿¡ ºñÇØ °³ÈÀϼö°¡ ±æ°í, ¿ÜÃÑÆ÷ÆíÀÇ ¹æÇâÀÌ ´Ù¸£¸ç ÅÐÀÇ À¯¹« ¶ÇÇÑ ´Ù¸¥ Ư¡ÀÌ ÀÖ¾ú´Ù. ll±º¿¡ ¼ÓÇϴ Ⱥ¹Îµé·¹´Â ´Ù¸¥ ¹Îµé·¹Á¾°ú´Â È»ö¿¡¼ È®¿¬ÇÑ Â÷À̸¦ º¸¿´À¸¸ç ÀÚ»ýÁ¾µé ¼Ó¿¡¼µµ ÀÙÀÇ ¹æÇâ°ú ¹ß¾ÆÀÇ »ý¸®Àû Ư¼º µî À¯ÀüÀûÀ¸·Î ¿©·¯ ´Ù¸¥ Á¡ÀÌ º¹ÇÕÀûÀ¸·Î ÀÛ¿ëÇÔÀ¸·Î¼ II±º³»¿¡¼µµ À¯ÀüÀû °Å¸®°¡ °¡Àå ¸Õ °ÍÀ¸·Î ³ªÅ¸³µ´Ù. |
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The genetic relationships between 4 Korean native Taraxacum and 2 naturalized Taraxacum species were analyzed using the random amplified polymorphic DNA (RAPD) method. Because 141 polymorphic bands were generated from 30 random primers selected through the primer screening, it was possible to analyze the genetic relationship among 6 Taraxacum species. In RAED with the primer OPC12, OPD16, OPK16, OPK17, OPK20, OPS1 or OPS8, many specific polymorphic bands have been appeared in each species. Especially RAPD with the primer OPS8, a specific polymorphic band at 564bp was appeared only in the naturalized Taraxacum officinale. Based on RAPD analysis, Korean native Taraxacum and naturalized Taraxacum species are divided into two groups. T. officinale and T. laevigatum are classified into group I which is a naturalized Taraxacum species group, and T. mongolicum, T. hallasanensis, T. ohwianum and T. coreanum are classified into group II which is a Korean native Taraxacum species group. The result from the RAPD method was very similar to the result from the Bootstrap method. From the examination of the physical characteristics of 6 Taraxacum species populated in Korea, flowering period of Taraxacum species in group I are longer than Taraxacum species in group ll, and the direction of involucral bract of Taruxacum species in the group I was also different comparing to the group ll. Because the flowering color, leaf direction, and the specificity of seed germination of T. coreanum were different compared to the other species in the group II, T. coreanum would be genetically divergent and showed the highest dissimilarity index score. |
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¹Îµé·¹¼Ó;À¯ÀüÀû ºÐ¼®;ºñÀ¯»çµµ °è¼ö;GENETIC RELATIONSHIP;TARAXACUM SPECIES;BOOTSTRAP; |
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Çѱ¹È¯°æ»ýÅÂÇÐȸÁö / v.17, no.2, 2003³â, pp.169-176
Çѱ¹È¯°æ»ýÅÂÇÐȸ
ISSN : 1229-3857
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200311921890550)
¾ð¾î : Çѱ¹¾î |
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³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø |
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