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Çѱ¹»ý¹°È¯°æÁ¶ÀýÇÐȸ / v.17, no.4, 2008³â, pp.297-305
ġĿ¸®(Cichorium intybus L.)¿Í ¾¦°«(Chrysanthemum coronarium L.)ÀÇ ±â³» Á¾ÀÚ¹ß¾Æ °³¼±
( Improvement of In Vitro Seed Germination in Chicory (Cichorium intybus L.) and Garland Chrysanthemum (Chrysanthemum coronarium L.) )
ȲÇöÁ¤;ÃÖ±Ù¿ø; °æÈñ´ëÇб³ »ý¸íÀÚ¿ø°úÇבּ¸¿ø;°æÈñ´ëÇб³ »ý¸íÀÚ¿ø°úÇבּ¸¿ø;
 
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ġĿ¸®¿Í ¾¦°«¿¡ ÀÖ¾î ±â³» ÆÄÁ¾ ½Ã ¹ß¾Æ ¹× ¹«±Õ¹¦ »ý»êÀ» ÁõÁø½Ã۱â À§ÇÑ ÀûÀýÇÑ ¹æ¹ýÀ» Á¦¾ÈÇϱâ À§ÇØ ÀÏ·ÃÀÇ ½ÇÇèµéÀÌ ¼öÇàµÇ¾ú´Ù. NaOClÀÇ ÀûÁ¤ ó¸®³óµµ¿Í ½Ã°£ °ËÁ¤À» ÅëÇØ ġĿ¸® 'Precole'°ú 'Chiavari' ¹× ¾¦°« 'Okiku 3' ¸ðµÎ Naocl 5%·Î 15ºÐ°£ ó¸®ÇÏ´Â °ÍÀÌ ¹ß¾Æ ¹× À¯¹¦Çü¼ºÀ» ÃËÁø½ÃŰ°í ¿À¿°¹ß»ýÀ» ¾ïÁ¦ÇÏ´Â °ÍÀ¸·Î °üÂûµÇ¾ú´Ù. ¹èÁöÁ¶¼º¿¡ ÀÖ¾î MS¿Í 1/2 MS󸮱¸µéÀÇ ¹ß¾Æ¹ÝÀÀ¼ºÀº ¶Ñ·ÇÀÌ Â÷À̰¡ ³ªÅ¸³ªÁö ¾Ê¾ÒÀ¸¸ç, sucrose ³óµµ°¡ ³ô¾ÆÁü¿¡ µû¶ó ¹ß¾Æ ¹× À¯¹¦Çü¼ºÀÌ Áö¿¬µÇ¾ú´Ù. ¹è¾ç¿ë±â¿¡ µû¸¥ ¹ÝÀÀ¼ºÀÇ Â÷À̴ ġĿ¸® 'Precole'ÀÇ °æ¿ì petri-dish¿¡¼­ ¾çÈ£ÇÑ ¹ß¾ÆÀ² ¹× À¯¹¦Çü¼ºÀ²À» º¸¿©ÁÖ¾úÁö¸¸, ġĿ¸® 'Chiavari'¿Í ¾¦°« 'Okiku 3'¿¡¼­´Â ¿ë±â¿¡ Å« ¿µÇâÀ» ¹ÞÁö ¾Ê¾Ò´Ù. ÃÊÀ½ÆÄ 󸮴 ġĿ¸®ÀÇ ¹ß¾ÆÀ² ¹× À¯¹¦Çü¼ºÀ²À» Áõ°¡½ÃÄ×Áö¸¸, ¾¦°« 'Okiku 3'¿¡¼­´Â ÃÊÀ½ÆÄ ó¸®È¿°ú°¡ ³ªÅ¸³ªÁö ¾Ê¾Ò´Ù. µû¶ó¼­ ġĿ¸®¿Í ¾¦°« ¸ðµÎ¿¡¼­ NaOCl 5%, 15ºÐ°£ Á¾ÀÚ¼Òµ¶Çϰí, sucrose°¡ ¹«Ã·°¡µÈ 1/2 MS ¹èÁö·Î petri-dish¸¦ ÀÌ¿ëÇϸç, ÃÊÀ½ÆÄ 󸮴 NaOCl 5% ¿ë¸Å¿¡¼­ 120ºÐ°£ ÇÏ´Â °ÍÀÌ ±â³» ¹ß¾ÆÇâ»ó¿¡ Àû´çÇÑ Á¶°ÇÀ¸·Î ¼±Á¤µÇ¾ú´Ù.
A series of experiments were conducted to suggest efficient in vitro germination conditions to improve germination rate and aseptic seedling production in chicory and garland chrysanthemum. For this purpose, various levels of NaOCl concentration and soaking treatment time combinations were tasted. Fifteen minutes of soaking treatment with 5% NaOCl solution significantly improved germination and seedling formation percentages and prevented contamination in both chicory 'Precole' and 'Chiavari' and garland chrysanthemum 'Okiku 3'. There was no significant difference in germination response between MS and 1/2 MS medium compositions, but germination and seedling formation were delayed as sucrose concentration increased. Although petri-dish among various culture containers gave rise to improved germination responses in chicory 'Precole', there was no significant difference in germination responses among culture container types in chicory 'Chiavari' and garland chrysanthemum 'Okiku 3'. The ultrasonic treatment stimulated germination and seedling formation in both chicory cultivars but there was no effect in garland chrysanthemum 'Okiku 3'. The results suggested 15 minutes with 5% NaOCl for seed sterilization, 1/2 MS medium with no sucrose and petri-dish as proper medium composition and culture container, and 120 minutes with 5% NaOCl solution of ultrasonic treatment as promoting method for in vitro germination in chicory and garland chrysanthemum.
 
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±â³»Á¾Àڹ߾Æ;¹è¾ç¿ë±â;¹èÁöÁ¶¼º;ÃÊÀ½ÆÄó¸®;culture container;in vitro germination;medium composition;NaOCl;ultrasonic treatment;
 
»ý¹°È¯°æÁ¶ÀýÇÐȸÁö / v.17, no.4, 2008³â, pp.297-305
Çѱ¹»ý¹°È¯°æÁ¶ÀýÇÐȸ
ISSN : 1229-4675
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200806942468214)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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