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Çѱ¹»ý¹°È¯°æÁ¶ÀýÇÐȸ / v.16, no.1, 2007³â, pp.54-61
³»Àú¿Â¼º°ú ¹Î°¨¼º È£¹Ú ǰÁ¾ÀÇ Àú¿Â ½ºÆ®·¹½º¿¡ ´ëÇÑ Ç×»êÈ­È¿¼ÒÀÇ È°¼º Â÷ÀÌ
( Changes in the Activities of Antioxidant Enzymes during Chilling Stress in Chilling-Tolerant and Chilling-Sensitive Cultivars of Cucurbita spp. )
°­³²ÁØ;±ÇÁر¹;Á¶¿ë¼·;ÃÖ¿µÇÏ; ¿ø¿¹¿¬±¸¼Ò ½Ã¼³¿ø¿¹½ÃÇèÀå;¿ø¿¹¿¬±¸¼Ò ½Ã¼³¿ø¿¹½ÃÇèÀå;¿ø¿¹¿¬±¸¼Ò ½Ã¼³¿ø¿¹½ÃÇèÀå;¿ø¿¹¿¬±¸¼Ò ½Ã¼³¿ø¿¹½ÃÇèÀå;
 
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Àú¿Â¿¡ ´ëÇÑ »ýÀ° ¹ÝÀÀÀÌ ´Ù¸¥ È£¹Ú µÎ ǰÁ¾ °£ Ç×»êÈ­È¿¼ÒÀÇ È°¼º º¯È­¸¦ ºÐ¼®ÇØ º» °á°ú, Àú¿Â¿¡ °­ÇÑ 'ÈæÁ¾'ÀÇ ÀÙ¿¡´Â RmÀÌ 0.20ÀÎ Mn-SOD¿Í ¿Í 0.52ÀÎ Fe-SOD°¡ ÁÖµÈ ¹êµå¿´°í, Àú¿Â¿¡ ¾àÇÑ 'Àç·¡ 13'È£ÀÇ ÀÙ¿¡´Â anÀÌ 0.20ÀÎ Mn-SOD¿Í 0.58ÀÎ Cu/Zn-SOD°¡ ÁÖµÈ ¹êµå¿´´Ù. Àú¿Â ó¸® ÈÄ 10Àϰ¿¡ 'ÈæÁ¾'ÀÇ ÀÙ¿¡´Â RmÀÌ 0.58ÀÎ Cu/Zn-SOD¹êµå°¡ ¹ßÇöµÇ¾ú°í, 'Àç·¡ 13È£'ÀÇ °æ¿ì ¹êµåÀÇ ¹Ðµµ°¡ Áõ°¡ÇÏ´Â °æÇâÀ» º¸¿´´Ù. APX ¹êµå ¹ßÇö ¾ç»óÀº µÎ ǰÁ¾ ¸ðµÎ Àû¿Â 󸮿¡¼­´Â Â÷À̰¡ ¾ø¾úÁö¸¸, Àú¿Â ó¸® ÈÄ 10ÀϰæºÎÅÍ Àú¿Â¿¡ °­ÇÑ 'ÈæÁ¾'ÀÇ ÀÙ¿¡¼­ »õ·Î¿î APX¹êµå°¡ ¹ßÇöµÇ¾ú´Ù. POB¹êµåÀÇ ¹ßÇö ¾ç»óÀº ǰÁ¾°£¿¡ ¶Ñ·ÇÇÑ Â÷À̰¡ ÀÖ¾ú´Âµ¥, Àû¿Â ÇÏ¿¡¼­ 'ÈæÁ¾'ÀÇ ÀÙ¿¡¼­´Â 4°³ÀÇ ÁÖµÈ ¹êµå°¡, 'Àç·¡ 13È£'ÀÇ ÀÙ¿¡¼­´Â ÇÑ °³ÀÇ ÁÖµÈ ¹êµå°¡ ¹ßÇöµÇ¾ú´Ù. ±×·¯³ª Àú¿Â 󸮽à 'ÈæÁ¾'ÀÇ ÀÙ¿¡¼­´Â RmÀÌ 0.36, 0.40 ¹× 0.54ÀÎ ¹êµåÀÇ ¹Ðµµ°¡ ±Þ°ÝÇÏ°Ô Áõ°¡ÇÑ ¹Ý¸é 'Àç·¡ 13È£'ÀÇ ÀÙ¿¡¼­´Â RmÀÌ 0.36°ú 0.54ÀÎ ¹êµåÀÇ ¹Ðµµ°¡ Áõ°¡ÇÏ¿´´Ù. Àú¿Â¿¡ ´ëÇÑ ³»¼º°ú °ü°è¾øÀÌ µÎ ǰÁ¾ ¸ðµÎ Àú¿Â ó¸® ÈÄ Ãʱ⿡´Â SOD, APX ¹× PODÀÇ È°¼ºÀÌ ±Þ°ÝÇÏ°Ô Áõ°¡ÇÏ´Â °æÇâÀ» º¸¿´Áö¸¸, Àú¿Â ó¸® ÈÄ ±â°£ÀÌ °æ°úÇÒ¼ö·Ï ǰÁ¾°£ Â÷À̰¡ ¶Ñ·ÇÇÏ¿´´Ù. 'ÈæÁ¾'ÀÇ ÀÙ¿¡¼­´Â ÀÌ·¯ÇÑ È°¼ºÀÌ Áö¼ÓÀûÀ¸·Î À¯ÁöµÇ¾úÁö¸¸, 'Àç·¡ 13È£'ÀÇ ÀÙ¿¡¼­´Â Àú¿Â ó¸® ÈÄ 5ÀϰæºÎÅÍ ±Þ°ÝÇÏ°Ô °¨¼ÒÇÏ¿© Àû¿Â 󸮱¸º¸´Ù ³·Àº Ȱ¼ºÀ» º¸¿© Àú¿Â¿¡ ´ëÇÑ Ç°Á¾°£ ³»¼ºÂ÷À̸¦ Àß ¹Ý¿µÇØ ÁÖ¾ú´Ù.
To determine whether antioxidant enzyme systems are related to chilling tolerance, changes of antioxidant enzyme activities during the chilling stress were determined in the leaves of a chilling-tolerant cultivar (Cucurbita ficifolia, cv. Heukjong) and a chilling-sensitive cultivar (Cucurbita moschata, cv. Jaerae 13). Leaves of chilling-tolerant plant have two major isoforms, Fe-SOD and Mn-SOD, at the Rm values of 0.20 and 0.52, respectively. In leaves of chilling-sensitive plant, two major isozymes of SOD was observed, one isoform is Mn-SOD at the Rm value of 0.20, and the other isoform is Cu/zn-SOD at the nm value of 0.58. When plants were treated with chilling stress, Cu/zn-SOD at the Rm value of 0.58 was newly expressed at 10 days after chilling stress in the chilling-tolerant plants, and density of this band increased at five days after chilling stress in the chilling-sensitive plants. One APX isozyme band was observed in unstressed plants of both cultivars. Under the chilling stress one APX isozyme band was newly expressed at 10 days after chilling stress in the chilling-tolerant cultivar. Significant genotype differences were observed fnr POD isozyme banding patterns such as few main isozyme bands in chilling-tolerant plants, and one band in chilling-sensitive plants. Densities of three POD isozyme bands at the Rm of 0.36, 0.40 and 0.54 increased at 10 days after chilling stress in the chilling-tolerant plants, while two bands at the nm of 0.36 and 0.54 increased at 10 days and 20 days after chilling stress in the chilling-sensitive plants, respectively. Activities of SOD, APX and POD significantly increased during five days after chilling stress in both cultivars. In the chilling-tolerant cultivar, activities of these enzymes were higher in chilling-stressed plant than in unstressed plants. However, activities of these enzymes in the chilling-sensitive cultivar decreased rapidly after five days of chilling stress, and were lower in chilling stressed plants than in unstressed plants.
 
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Àú¿Â;ascorbic peroxidase;chilling;peroxidase;superoxide dismutase;
 
»ý¹°È¯°æÁ¶ÀýÇÐȸÁö / v.16, no.1, 2007³â, pp.54-61
Çѱ¹»ý¹°È¯°æÁ¶ÀýÇÐȸ
ISSN : 1229-4675
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200714539166376)
¾ð¾î : ¿µ¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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