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Çѱ¹»ý¹°È¯°æÁ¶ÀýÇÐȸ / v.16, no.2, 2007³â, pp.121-129
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( Differential Responses of Antioxidant Enzymes on Chilling and Drought Stress in Tomato Seedlings (Lycopersicon esculentum L.) )
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Àú¿Â ¶Ç´Â °ÇÁ¶ 󸮿¡ µû¸¥ Å丶Åä À¯¹¦ÀÇ »ýÀ°°ú ºÎÀ§º° Ç×»êÈ­È¿¼ÒÀÇ ¹ÝÀÀ ¾ç»óÀ» ºÐ¼®ÇÑ °á°ú, Å丶Åä À¯¹¦ÀÇ »ýüÁßÀº ó¸® ÈÄ 12Àϰ¿¡ ´ëÁ¶±¸¿¡ ºñÇØ °¢°¢ 69.5% ¿Í 50.6% °¨¼ÒÇÏ¿´´Ù. SOD¿Í PODÀÇ È°¼ºÀº ´ëÁ¶±¸¿¡ ºñÇØ Àú¿Â ¶Ç´Â °ÇÁ¶ 󸮿¡¼­ ³ôÀº Ȱ¼ºÀ» º¸¿´´Âµ¥, Àú¿Â 󸮽ÿ¡´Â »Ñ¸®¿¡¼­ ´õ ³ôÀº Ȱ¼ºÀ» º¸¿´°í °ÇÁ¶ 󸮿¡¼­´Â ÀÙ°ú Áٱ⿡¼­ ³ôÀº Ȱ¼ºÀ» º¸¿´´Ù. ÀÌ·¯ÇÑ °á°ú´Â µ¿À§È¿¼ÒÀÇ ¹ßÇö¾ç»ó¿¡¼­µµ ÀÏÄ¡ÇÏ¿´´Ù. GRÀÇ È°¼ºÀº Àú¿Â ¶Ç´Â °ÇÁ¶ 󸮽à ´ëÁ¶±¸º¸´Ù ³ôÀº Ȱ¼ºÀ» º¸¿´´Âµ¥, ÀÙ°ú Áٱ⿡¼­´Â Àú¿Â°ú °ÇÁ¶ 󸮰£ÀÇ Â÷ÀÌ´Â ¾ø¾úÁö¸¸, »Ñ¸®¿¡¼­´Â °ÇÁ¶ 󸮰¡ ³ôÀº °æÇâÀ» º¸¿´´Ù. GR µ¿À§È¿¼Ò ¹ßÇö¾ç»óÀº Àú¿Â°ú °ÇÁ¶Ã³¸®½Ã¿¡´Â GR-3 ¹êµå°¡ ÀÙ¿¡¼­´Â ¹ßÇöµÇ¾î ´ëÁ¶±¸¿Í Â÷À̰¡ ÀÖ¾úÁö¸¸, ÁÙ±â¿Í »Ñ¸®¿¡¼­´Â Å« Â÷À̰¡ ¾ø¾ú´Ù. PPO Ȱ¼ºÀº ÀÙ¿¡¼­´Â ¸ðµç 󸮿¡¼­ Â÷À̰¡ ¾ø¾úÁö¸¸, ÁÙ±â¿Í »Ñ¸®¿¡¼­´Â Àú¿Â ¶Ç´Â °ÇÁ¶ 󸮿¡¼­ ´ëÁ¶±¸º¸´Ù ³ôÀº °æÇâÀ» º¸¿´´Ù. ƯÈ÷ ÁÙ±âÀÇ PPO Ȱ¼ºÀº Àú¿Â 󸮺¸´Ù °ÇÁ¶Ã³¸®¿¡¼­ ³ô¾Ò°í »Ñ¸®ÀÇ PPO Ȱ¼ºÀº °ÇÁ¶ 󸮺¸´Ù Àú¿Â 󸮿¡¼­ ³ô¾Ò´Ù. µ¿À§È¿¼ÒÀÇ ¹ßÇö¾ç»ó¿¡¼­µµ °ÇÁ¶Ã³¸®¿¡¼­´Â Áٱ⿡¼­, Àú¿Â 󸮿¡¼­´Â »Ñ¸®¿¡¼­ ³ôÀº ¹Ðµµ¸¦ º¸¿© ºÒ·® ȯ°æ¿¡ µû¸¥ ºÎÀ§º° ¹ÝÀÀ Â÷À̸¦ Àß ¹Ý¿µÇØ ÁÖ¾ú´Ù.
Responses of antioxidant enzymes on chilling and drought stress in tomato seedlings were investigated. Growing patterns of tomato based on fresh weight of tomato seedlings were severely affected by chilling and drought stress. Fresh weight of tomato seedlings were reduced by 69.5% in chilling stress and 50.6% in drought stress compared to those in the unstressed control seedlings after 12 days of stress. The specific and gel activity of SOD and POD in the leaves, shoots, and roots of tomato seedlings were significantly increased by chilling and drought stress. Activation of SOD and POD activity by chilling stress were higher in the roots than those of drought stress. However, activation of SOD and POD activity by drought stress were higher in the leaves and shoots than those of chilling stress. The specific and gel activity of GR in the leaves, shoots, and roots of tomato seedlings were also significantly increased by chilling and drought stress. When the seedlings were treated with chilling or drought stress, one GR isozyme band (GR-3) was newly expressed in the leaves of tomato seedlings. The specific and gel activity of PPO was significantly increased in the roots and shoots of tomato seedlings by chilling and drought stress, respectively. However, the specific and gel activity of PPO in the leaves is no difference between stressed and controlled tomato seedlings.
 
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°ÇÁ¶;Àú¿Â;Å丶Åä;chilling;drought;GR;POD;PPO;SOD;tomato;
 
»ý¹°È¯°æÁ¶ÀýÇÐȸÁö / v.16, no.2, 2007³â, pp.121-129
Çѱ¹»ý¹°È¯°æÁ¶ÀýÇÐȸ
ISSN : 1229-4675
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200725522698692)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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