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Çѱ¹»ý¹°È¯°æÁ¶ÀýÇÐȸ / v.4, no.2, 1995³â, pp.136-143
Àú¿Â ȯ°æÀÌ Canola ¿øÇüÁú¸·ÀÇ Fatty Acid ±¸¼º¿¡ ¹ÌÄ¡´Â ¿µÇâ
( Effect of Low Temperature upon the Fatty Acid Composition Plasma Membrane of Canola )
±Ç¼ºÈ¯;;ÀüÈñ;±èÀçö; ¹Ì³×¼ÒŸÁÖ¸³´ë;¹Ì³×¼ÒŸÁÖ¸³´ë;¿ø¿¹¿¬±¸¼Ò;ÀüºÏ´ëÇб³ ¿ø¿¹Çаú;
 
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Canola(Brassica napus) ¿±¿¡¼­ ÃßÃâÇÑ ¹Ì¼¼¸·À¸·ÎºÎÅÍ PEG-dextran 2»óºÐȹ¹ýÀ» ÀÌ¿ëÇÏ¿© ¼¼Æ÷¸·°ú ¼¼Æ÷³»¸·À» ºÐ¸®ÇÏ¿´´Ù. U$_2$ »ó¿¡ ÀÖ´Â ¿øÇüÁú¸·ÀÇ $K^{+}$-ATPaseÀÇ Æ¯ÀÌȰ¼ºµµ°¡ ¹Ì¼¼¸·¿¡ ºñÇÏ¿© $25^{circ}C$¿¡¼­ ÀÚ¶õ canola´Â 6.6¹è, 1$0^{circ}C$¿¡¼­ 4.6¹è °¢°¢ Áõ°¡µÇ¾ú´Ù. ¿øÇüÁú¸·(U$_2$)Àº ¹Ì¼¼¸·À̳ª ¼¼Æ÷³»¸·(L$_2$) º¸´Ù cytochrome-c-oxidase Ȱ¼ºÀÌ Àû°Ô ³ªÅ¸³­ ¹Ý¸é, ¼¼Æ÷³»¸·¿¡¼­´Â $K^{+}$-ATPaseÀÇ Æ¯ÀÌȰ¼ºµµ°¡ °¡Àå Àû°Ô ³ªÅ¸³µ´Ù. 1$0^{circ}C$¿¡¼­ »ýÀåÇÑ canolaÀÇ 18:3/18:2 ·üÀº $25^{circ}C$º¸´Ù 29.2% ´õ ³ô°Ô ³ªÅ¸³µ´Ù. ¿øÇüÁú¸·ÀÇ 2Áß°áÇÕÁö¼ö´Â 1$0^{circ}C$¿¡¼­ »ýÀåÇÑ canola°¡ $25^{circ}C$¿¡¼­ »ýÀåÇÑ °Íº¸´Ù 8.9% ´õ Áõ°¡µÇ¾úÀ¸¸ç ¼¼Æ÷³»¸·¿¡¼­µµ °°Àº °æÇâÀ¸·Î½á 1$0^{circ}C$¿¡¼­ 19.7% ´õ Áõ°¡µÇ´Â Çö»óÀ» º¸¿´´Ù. ¶ÇÇÑ ¿±·Ï¼Ò ÇÔ·®Àº 1$0^{circ}C$¿¡¼­ »ýÀåÇÑ °ÍÀÌ $25^{circ}C$¿¡ ºñÇÏ¿© 17.3% ³·¾Ò´Ù. Canola°¡ Àú¿Â¿¡¼­ »ýÀå½Ã ÁÖ·Î $C_{18}$ Áö¹æ»êµéÀÌ º¯È­µÇ¾î, ¼¼Æ÷¸· ³»¿¡ ºÒÆ÷È­ Áö¹æ»êÀÌ ¸¹¾ÒÀ¸¸ç, ±× Áß¿¡¼­µµ ¸®·Ñ·»»ê(18:3)ÀÌ Å©°Ô º¯È­µÇ´Â Çö»óÀ» º¸¿´´Ù. ÀÌ·¯ÇÑ º¯È­´Â »ý¸®ÀûÀ¸·Î canolaÀÇ ¼¼Æ÷¸·ÀÌ Àú¿Â¿¡ »ì¾Æ°¡±â À§ÇÑ ÇϳªÀÇ ¼ö´ÜÀ¸·Î ÃßÁ¤µÈ´Ù.
Using a PEG- dextran two phase partition method, plasma and intracellular membrane separated from microsomal membrane of canola (Brassica napus) leaves have been fractionated by centrifugation. $K^{+}$- ATPase specific activity in the plasma membrane (U$_2$ phase) of plants grown at $25^{circ}C$ and 1$0^{circ}C$ were 6.6 and 4.6 times, respectively that of the microsomal membrane. Plasma membrane had a lower cytochrome- c- oxidase specific activity than the microsomal membrane or intracellular membrane, while intracellular membrane (L$_2$ phase) had a high cytochrome-c- oxidase but little $K^{+}$- ATPase specific activity. The plasma membrane of canola grown at 1$0^{circ}C$ had higher 18:3 to 18:2 (linolenic to linoleic acid) ratio (29.2% ) and higher degree of unsaturation than that grown at $25^{circ}C$ The double bond index of plasma membrane from canola grown at 1$0^{circ}C$ increased by 8.9% relative to canola grown at $25^{circ}C$. Similar, intracellular membrane increased by 19.7% at 1$0^{circ}C$. Canola grown at 1$0^{circ}C$ was lower in chlorophyll contents (17.3%) than that grown at $25^{circ}C$. These changes in fatty acid unsaturation were attributable largely to change in Cl8 fatty acid, with major changes occurring in linolenic acid (18 :3) which might have a physiological role of membrane to adaptation on low temperature.ure.
 
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Ä«³î¶ó;2»óºÐ¸®;¿øÇüÁú¸·;¼¼Æ÷³»¸·;¹Ì¼¼¸·;Áö¹æ»ê;ÀÌÁß°áÇÕÁö¼ö;canola;two phase partition;plasma membrane;intracelluar membrane;microsomal membrane;fatty acid;double bond index.;
 
»ý¹°È¯°æÁ¶ÀýÇÐȸÁö / v.4, no.2, 1995³â, pp.136-143
Çѱ¹»ý¹°È¯°æÁ¶ÀýÇÐȸ
ISSN : 1229-4675
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199511922404598)
¾ð¾î : Çѱ¹¾î
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