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Çѱ¹»ý¹°È¯°æÁ¶ÀýÇÐȸ / v.11, no.3, 2002³â, pp.115-120
½ºÅ¸Æ¼½º ¡®¿À¼Ç ºí·ç¡¯ÀÇ ÀÚ°¡¿µ¾ç¹è¾ç½Ã ±¤µµ, ȯ±âȽ¼ö ¹× $ extrm{CO}_2$ ³óµµ°¡ ¼Ò½Ä¹°Ã¼ÀÇ ±â³» »ýÀ°¿¡ ¹ÌÄ¡´Â ¿µÇâ
( Autotrophic Growth of Limonium spp. 'Ocean Blue' Plantlets In Vitro as Affected by PPF, NAEH and $ extrm{CO}_2$ Concentration )
Á¤±â¿ø;Á¤º´·æ; °æ»ó´ëÇб³ ÀÀ¿ë»ý¸í°úÇкÎ;°æ»ó´ëÇб³ ³ó¾÷»ý¸í°úÇבּ¸¿ø;
 
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ÀÚ°¡¿µ¾ç¹è¾ç½Ã ±¤µµ,ȯ±âȽ¼ö ¹× $CO_2$³óµµ°¡ ½ºÅ¸Æ¼½º ¡®Ocean Blue¡¯ ¼Ò½Ä¹°Ã¼ÀÇ »ýÀå¿¡ ¹ÌÄ¡´Â ¿µÇâÀ» Á¶»çÇÏ¿´´Ù MS ¹èÁö¿¡¼­ ±¤ÈçÇÕ¿µ¾çÇØ¾çÀ» ÅëÇÏ¿© Áõ½ÄµÈ ½ºÅ¸Æ¼½º ½Ä¹°Ã¼¿¡¼­ 2~3ÀåÀÇ ÀÙÀ» °¡Áø ÁÙ±â ÀýÆíü¸¦ äÃëÇÏ¿© ¹è¾ç¿ë±â´ç 4°³¾¿ Ä¡»óÇÏ¿´´Ù. ÀýÆíü´Â ¿Âµµ 25$pm$1$^{circ}C$. »ó´ë½Àµµ 70~ 80%ÀÎ ¹è¾ç½Ç¿¡¼­ cool-white Çü±¤·¥ÇÁ·Î ¸í±âµ¿¾È 70, 150, ¶Ç´Â 220$mu$mol. $m^{-2}$ . $s^{-1}$ÀÇ ±¤À» °ø±ÞÇϸ鼭 41ÀÏ µ¿¾È ¹è¾çÇÏ¿´´Ù. MS ±âº»¹èÁö¸¦ »ç¿ëÇÏ¿´À¸¸ç, sucrose¿Í vitaminÀº ÷°¡ÇÏÁö ¾Ê¾Ò´Ù. ±¤µµ°¡ ³ô¾ÆÁú¼ö·Ï »ýü Áß,°Ç¹°Áß ¹× ¿±¸éÀûÀÌ Áõ°¡ÇÏ¿´´Ù. $CO_2$³óµµ¸¦ ³ô¿©ÁØ °æ¿ì ÃÊÀå, »ýüÁß, °Ç¹°Áß ¹× ¿±¸éÀûÀÌ Áõ°¡ÇÏ¿´´Ù. »Ñ¸®±æÀÌ¿Í ¿±·Ï¼Ò ³óµµ´Â ±¤µµ³ª $CO_2$ÀÇ ¿µÇâÀ» ¹ÞÁö ¾Ê¾Ò´Ù. ¹è¾ç¿ë±â ³»ÀÇ È¯±âȽ¼ö¸¦ ³ôÀ̰í, °í±¤µµÀÇ $CO_2$¸¦ °ø±ÞÇÒ¼ö·Ï,±×¸®°í PPF¸¦ Áõ°¡½Ãų¼ö·Ï ½ºÅ¸Æ¼½º ¼Ò½Ä¹°Ã¼ÀÇ ÀÚ°¡¿µ¾ç¹è¾ç¿¡¼­ÀÇ »ýÀ°ÀÌ ÃËÁøµÇ¾ú´Ù.
Growth and development of Limonium spp.¡®Ocean Blue¡¯plantlets were studied under three levels of photosynthetic photon flux (PPF),70,150 and 220 $mu$mol. $m^{-2}$ . $s^{-1}$ , two levels of $CO_2$ concentration, 500 and 1000 $mu$mol. $m^{-1}$ , and two levels of number of air exchanges per hour (NAEH),0.1 $h^{-1}$ and 2.8 $h^{-l}$. Explants were obtained from photomixotrophically-micropropagated plantlets. Four explants per vessel were cultured under cool-white fluorescent lamps for 16 h. $d^{-1}$ at 25$pm$11$^{circ}C$ and 70~80% relative humidity. In treatments of 2.8 $h^{-1}$ NAEH, a 10 mm round hole made on the vessel cap was sealed with a microporous filter and two $CO_2$ concentrations in the culture rooms were provided from a liquefied $CO_2$ tank. Fresh and dry weights, height, length of the longest root, number of loaves, and leaf area significantly increased with increasing PPF and especially, $CO_2$ concentration. Growth was enhanced by a 2.8 $h^{-1}$ NAEH. Overall, treatment with a 220 $mu$mol. $m^{-2}$ . $s^{-1}$ PPF and a 1000 $mu$mol. $m^{-1}$ $CO_2$ resulted in the most vigorous growth of Limonium spp. ¡®Ocean Blue¡¯ plantlets.s.
 
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¹Ì¼¼Áõ½Ä;¹è¾çȯ°æ;±¤µµ;Åë±â;culture environment;light intensity;micropropagation;ventilation;
 
»ý¹°È¯°æÁ¶ÀýÇÐȸÁö / v.11, no.3, 2002³â, pp.115-120
Çѱ¹»ý¹°È¯°æÁ¶ÀýÇÐȸ
ISSN : 1229-4675
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200211922407202)
¾ð¾î : Çѱ¹¾î
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