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Çѱ¹»ýÅÂÇÐȸ / v.22, no.1, 1999³â, pp.51-58
Allelochemicals ÇÔÀ¯ ½Ä¹°ÀÇ Ç×±Õ È¿°ú
( Antimicrobial Activity of Some Plants Containing Allelochemicals )
ÀÌÈ£ÁØ;±è¿ë¿Á; °Ç±¹´ëÇб³ ÀÚ¿¬°úÇкΠ»ý¸í°úÇÐÀü°ø;°Ç±¹´ëÇб³ ÀÚ¿¬°úÇкΠ»ý¸í°úÇÐÀü°ø;
 
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ÀÚ»ý½Ä¹°°ú ±ÍÈ­½Ä¹°À» ´ë»óÀ¸·Î allelochemicals¸¦ ºÐ¼®ÇÏ°í ±× Ç×±ÕÈ¿°ú¸¦ Á¶»çÇÏ¿´´Ù. ÀÚ»ý½Ä¹°°ú ±ÍÈ­½Ä¹° ÃßÃâ¾× °£ÀÇ °¡¿ë¼º °íÇü¹° ÇÔ·®¿¡´Â Â÷À̸¦ º¸ÀÌÁö ¾Ê¾Ò°í ÀÚ»ý½Ä¹°ÀÎ ±î¸¶ÁßÀÇ °¡¿ë¼º °íÇü¹° ÇÔ·®ÀÌ 90 mg/ml·Î °¡Àå ³ô°Ô ³ªÅ¸³µ´Ù. ÀÚ»ý½Ä¹°ÀÇ ¿¡ÅºÀ» ÃßÃâ¾×¿¡ ÀÇÇÑ Aspergillus phoenicis KCTC 1228ÀÇ Ç×±Õ·ÂÀº ±î¸¶Áß¿¡¼­¸¸ ³ªÅ¸³µÀ¸¸ç clear zoneÀÌ 18 mm¿´°í Æ÷ÀÚÇü¼ºÀº ¾ïÁ¦µÇ¾ú´Ù. ±ÍÈ­½Ä¹° Áß µÅÁöÇ®°ú ¸ÁÃÊ¿¡¼­ Æ÷ÀÚÇü¼ºÀÌ ¾ïÁ¦µÇ¸é¼­ clear zoneÀÌ 24 mm¿Í 22 mm·Î Çü¼ºµÇ¾ú°í ¹Ì±¹ÀÚ¸®°ø°ú ¿øÃßõÀα¹Àº Æ÷ÀÚ°¡ Çü¼ºµÇ¸é¼­ clear zoneÀÌ 22 mm¿Í 19 mm·Î ³ªÅ¸³µ´Ù. ÀÚ»ý½Ä¹°ÀÎ ±î¸¶Áß°ú ±ÍÈ­½Ä¹°ÀÎ ¹Ì±¹ÀÚ¸®°ø°ú µÅÁöÇ®¿¡¼­ Åä¾ç¹Ì»ý¹°ÀÎ Bacillus sphiaericus 2362¿Í Bacillus sphiaericus 2291, Bacillus thuringiensis var. subtilis¿Í Bacillus thuringiensis var. cereus¿¡¼­ Ç×±Õ·ÂÀÌ ³ªÅ¸³µ´Âµ¥ µÅÁöÇ®ÀÌ °¢ ±ÕÁÖ¿¡¼­ Ç×±ÕȰ¼ºÀÌ °¡Àå Å©°Ô ³ªÅ¸³µ´Ù. ƯÈ÷ Bacillus thuringiensis var. subtilis¿¡¼­ clear zoneÀÌ 32 mm·Î Ȱ¼ºÀÌ °­ÇÏ°Ô Á¶»çµÇ¾ú´Ù. ÀϹÝÀûÀ¸·Î ÃßÃâ¾×³»ÀÇ °¡¿ë¼º °íÇü¹° ÇÔ·®ÀÌ ³ôÀ»¼ö·Ï Ç×±Õ È°¼ºÀÌ Å©°Ô ³ªÅ¸³²À» ¾Ë ¼ö ÀÖ´Ù. ÀÚ»ý½Ä¹°ÀÎ ±î¸¶Áß°ú ±ÍÈ­½Ä¹°ÀÎ ¹Ì±¹ÀÚ¸®°ø°ú µÅÁöÇ®ÀÇ ÃßÃâ¾×À» HPLC·Î ¼ººÐºÐ¼®ÇÑ °á°ú ±î¸¶ÁßÀº hydroquinone¿Ü 3Á¾ÀÌ, ¹Ì±¹ÀÚ¸®°øÀº cinnamic acid¿Ü 5Á¾ÀÌ, µÅÁöÇ®Àº benzoic acid¿Ü 7Á¾ÀÌ ºÐ¼®µÇ¾ú´Ù. Phenolic compound °¢°¢¿¡ ´ëÇÑ Aspergillus phoenicis KCTC 1228¿¡ ´ëÇÑ Ç×±Õ·ÂÀº coumaric acid¿Í benzoic acid¿¡¼­ Á¶»çµÇ¾ú´Ù.
Korean native plants and naturalized plants were analyzed for allelochemicals, and their antimicrobial effects were studied. The difference in soluble solid contents between Korean native plants and naturalized species was not significant, and the Korean native plant, Solanum nigrum showed the highest soluble solid content of 90 mg/ml. The ethanol extract of the Korean native plant, Solanum nigrum showed antifungal activity to Aspergillus phoenicis KCTC 1228, with a clear zone of 18 mm, and spore formation was not observed from the treatment. The naturalized plants Ambrosia artemisiifolia var. elatior and Erigeron canadensis showed inhibition of spore formation and the clear zones were at 24 mm and 22 mm, respectively. The clear zones of Aspergillus phoenicis KCTC 1228 treated with ethanol extrats of Phytolacca americana and Rudbeckia bicolor were 22 mm and 19 mm, respectively, and spore formation was observed from the treatment. The Korean native plant, Solanum nigrum and naturalized plants, Phytolacca americana and Ambrosia artemisiifolia var. elatior showed antimicrobial activity against Bacillus sphiaericus 2362, and Bacillus sphiaericus 2297, Bacillus thuringiensis var. subtilis and Baicillus thuringiensis var. cereus. The antimicrobial activity of Ambrosia artemisiifolia var. elatior showed the largest clear zone of 32 mm against Bacillus thuringiensis var. subtilis. In general, the more soluble the solid contents of the extracts, the greater were the antifungal and antimicrobial activities. The phenolic compounds from the Korean native plant, Solanum nigrum and the naturalized species, Phytolacca americana and Ambrosia artemisiifolia var. elatior were analyzed by high performance liquid chromatography. Three phenolic compounds including hydroquinone were identified in Solanum nigrum. In contrast, five and seven phenolic compounds were identified in Phytolacca americana and Ambrosia artemisiifolia var. elatior, respectively. The antifungal activity against Aspergillus phoenicis KCTC 1228 was found to be due to the coumaric and benzoic compounds.
 
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The Korean Journal of Ecology / v.22, no.1, 1999³â, pp.51-58
Çѱ¹»ýÅÂÇÐȸ
ISSN : 1225-0317
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199911922412827)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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