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Çѱ¹»ýÅÂÇÐȸ / v.22, no.1, 1999³â, pp.45-50
±èÄ¡ ¼­½Äó¿¡¼­ Listeria monocytogenes¸¦ ¾ïÁ¦ÇÏ´Â lactococciÀÇ ºÐ¸®¿Í 16S rDNAºÐ¼®¿¡ ÀÇÇÑ µ¿Á¤
( Isolation of Lactococci Inhibiting Listeria monocytogenes from Kimchi Habitat and Its Identification by 16S rDNA Analysis )
¹ÚÀºÁÖ;ÇÑÈ«ÀÇ;¹ÎºÀÈñ; ÀÎÇÏ´ëÇб³ À̰ú´ëÇÐ »ý¹°ÇؾçÇкÎ;ÀÎÇÏ´ëÇб³ À̰ú´ëÇÐ »ý¹°ÇؾçÇкÎ;´ë±¸´ëÇб³ ÀÚ¿¬°úÇдëÇÐ »ý¹°Çаú;
 
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±èÄ¡ ¹ßÈ¿ Ãʱ⿡ bacteriocin »ý¼º À¯»ê±ÕÀ» ºÐ¸®ÇϰíÀÚ ÇÏ¿´´Ù. ºÐ¸® À¯»ê±ÕÀº ÇüÅÂ, ¹è¾ç ¹× »ý¸®ÇÐÀûÀΠƯ¡°ú 16S rDNAÀÇ ºÎºÐÀûÀÎ ¿°±â¼­¿­·ÎºÎÅÍ Lactococcus lactis·Î µ¿Á¤µÇ¾ú´Ù. ºÐ¸®±ÕÁÖ°¡ »ý¼ºÇÑ bacteriocinÀº Listeria monocytogenes, Staphylococcus aureus¿Í °°Àº ±×¶÷ ¾ç¼º º´¿ø¼º ¼¼±Õ°ú ¸î¸î À¯»ê±Õ¿¡ ´ëÇØ Ç×±ÕȰ¼ºÀÌ ÀÖ¾ú°í, ±×¶÷À½¼º±ÕÀÎ Yesinia¿¡´Â Ȱ¼ºÀÌ ¾ø¾ú´Ù. bacteriocinÀÇ È°¼ºÀº protease, protease ¥¹¥³, a-chymotrypsin°ú pepsin¿¡ ´ëÇØ¼­ Ȱ¼ºÀÌ ¼Ò½ÇµÇ¾úÀ¸³ª, lipase, trypsin, lysozyme¿¡ ´ëÇØ¼­´Â Ȱ¼ºÀÌ À¯ÁöµÇ¾ú´Ù. bacteriocinÀÇ È°¼ºÀº pH 2¡­11¿¡¼­ ¾ÈÁ¤ÀûÀ̸ç l00¡É¿¡¼­ 10ºÐ°£ ¿­Ã³¸®½Ã¿¡µµ º¯ÇÏÁö ¾Ê¾Ò´Ù. µû¶ó¼­ L. monocytogenes´Â ¹ßÈ¿Ãʱ⿡ L. lactis¿¡ ÀÇÇÏ¿© ¾ïÁ¦µÉ ¼ö ÀÖ´Ù.
A bacteriocin-producing strain was isolated from kimchi at the early stage of kimchi fermentation. It was identified as Lactococcus lactis by morphological, cultural and physiological characteristics and partial sequence of 16S rDNA. The bacteriocin from isolate had antimicrobial activity against gram positive pathogenic bacteria, such as Listeria monocytogenes. Staphylococcus aureus and several strains of lactic acid bacteria but not to gram negative bacteria, Yersinia enterocolitica. The bacteriocin was sensitive to protease, protease ¥¹¥³, a-chymotrypsin and pepsin but not to lipase, trypsin and lysozyme. The bacteriocin activity was stable at pH 2-11 and temperature of 100 for 10 min. Thus, Listeria monocytogenes could be inhibited by Lactococcus lactis at early stage of fermentation.
 
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±èÄ¡;16S rDNA ¿°±â¼­¿­;Bacteriocin;Kimchi;Lactococcus lactis;Listeria monocytogenes;Sequence of 16S rDNA.;
 
The Korean Journal of Ecology / v.22, no.1, 1999³â, pp.45-50
Çѱ¹»ýÅÂÇÐȸ
ISSN : 1225-0317
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199911922412807)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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