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한국생태학회 / v.20, no.3, 1997년, pp.169-173 
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Anabaena flos-aquae 에서의 세포사멸계수(Cell Death Constant)의 측정
( Measurement of Cell Death Constant in Anabaena flos-aquae (Cyanophyceae) by the Molecular Probe ) |
| 오인혜; 배재대학교 생물학과;
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| 초 록 |
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| The measurement of cell death constant in Anabaena flos-aquae was tested by the Live/Dead BacLight Viability kit(Molecular Probes Co., Seatle, WA). When the Live/Dead BacLight Viability kit was applied to Anabaena flos-aquae, the cells with intact cell membranes(live cells) stained fluorescent green, while the cell with damaged membranes(dead cells) stained fluorescent red and the background remained virtually nonfluorescent. The rations of live : dead cells in the cell suspension were controlled artifically and Live/Dead BacLight Viability kit was applied to them. The ratios of green:red fluorescent cells in the cell suspension were the same as those of live : dead cells controlled artifically. It was also approved by the fluorescence emission. The cell death constant was measured in the P-limited Anabaena flos-aquae chemostal culture in the N-fixing and $KNO_3-supplied$ conditions. The culture in N-fixing chemostat had a dead cell proportion of 1.2% at the growth rate of 0.7/day and increased to 2.6% at the growth rate of 0.3/day. The cell death constant of N-fixing culture was 0.008/day.There was a same trend in the $KNO_3-supplied$ chemostat culture. The proportion of dead cell was 1.6% of dead cell proportion at the growth rate of 0.7/day and increased to 4.3% at the growth rate of 0.3/day. |
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| 키워드 |
| Anabaena flos-aquae;Cell death constant;BacLight Viability Kit;Chemostat; |
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The Korean Journal of Ecology / v.20, no.3, 1997년, pp.169-173
한국생태학회
ISSN : 1225-0317
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199711919962586)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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