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한국생태학회 / v.21, no.1, 1998년, pp.35-45 
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미국자리공 (Phytolacca americana)에서 추출한 생물활성 물질의 동정 및 생물검정에 관 하여
( Identification and Bioassay of Bioactive Compounds Isolated from Phytolacca americana ) |
| 한상미;최관삼;배기환; 충남대학교 응용생물화학부;충남대학교 응용생물화학부;충남대학교 약학과;
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| 초 록 |
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| This study was carried out for the identification and bioassay of bioactive compounds which isolated from Phyutolacca americana L. obtained results are summarized as follows: 1. Two biological active compounds were found from crude extracts of P. americana roots, using the systematic solvent fractionations, such as ethyl acetate fraction and butanol fraction. One biological compound was isolated from the ethyl acetate fraction with silicagel column chromatography, which was identified as a ${alpha}-spinasterol$ by spectral analysis of IR, H-NMR, C-NMR and MS. The other one is isolated from butanol fraction which was identified as phytolaccoside E by spectral analysis of IR, H-NMR, C-NMR and MS. 2. The ${alpha}-spinasterol$ and phytolaccoside E induced necrosis of primary root and resulted in death of the tested plant. These two compounds strongly inhibited to the growth of Mucor racemous and Phytophthora infestants but did not inhibited the growth of Colletotricum lagenarium and Fusarium oxisporum. 3. Cytotoxicity of the two biological active compound was exammined to the two different animal cancer cell lines (L1210, K562). The phytolaccoside E has some cytotoxical activity to the growth of cancer cell lines (L1210, K562) but $alpha$-spinasterol has not cytotoxical effect. |
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| 키워드 |
| Extraction of biological active compound;${alpha}$-Spinasterol;Phytolaccoside E;Bioassay; |
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The Korean Journal of Ecology / v.21, no.1, 1998년, pp.35-45
한국생태학회
ISSN : 1225-0317
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199811919962944)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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