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Çѱ¹»ýÅÂÇÐȸ / v.22, no.5, 1999³â, pp.305-309
ÇÏõ¿¡¼­ tetQ¿Í aacC2 À¯ÀüÀÚÀÇ ºÐÆ÷ ¾ç»ó
( Distributional Pattern of tetQ and aacC2 genes in Stream Water )
Á¤À缺;ÀÌ¿µÁ¾;±èÁ¾È«; ¼øÃµ´ëÇб³ ÀÚ¿¬°úÇдëÇÐ »ý¹°Çаú;¼øÃµ´ëÇб³ ÀÚ¿¬°úÇдëÇÐ »ý¹°Çаú, ±¤ÁÖº¸°Ç´ëÇÐ ÀÓ»óº´¸®°ú;¼øÃµ´ëÇб³ ÀÚ¿¬°úÇдëÇÐ »ý¹°Çаú;
 
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ÇÏõ¿¡¼­ tetracycline°ú gentamicin ÀúÇ×¼º À¯ÀüÀÚÀÎ tetQ¿Í aacC2ÀÇ ºÐÆ÷¸¦ ¾Ë¾Æº¸±â À§ÇØ ¼øÃµÁö¿ªÀÇ ÇÏõ¼ö·ÎºÎÅÍ Àüü ¼¼±Õ±ºÁýÀÇ DNA¸¦ ºÐ¼®ÇÏ¿´´Ù. ¹è¾çµÇÁö ¾Ê´Â ¼¼±ÕÀÇ ÀúÇ×¼ºÀ» °í·ÁÇÏ¿© 1literÀÇ ÇÏõ¼ö¿¡ µé¾î ÀÖ´Â Àüü ¼¼±ÕÀÇ DNA¸¦ freeze-thaw ¹æ¹ýÀ¸·Î ÃßÃâÇÏ¿© PCRÀ» ÅëÇØ Ç¥Àû À¯ÀüÀÚÀÇ ÃâÇö Á¤µµ¸¦ Á¶»çÇÏ¿´´Ù. ±× °á°ú tetQ À¯ÀüÀÚ´Â Ãà»ê³óÀåÀÌ ÀÖ´Â Á¦ 1ÁöÁ¡¿¡¼­ °¡Àå ¸¹Àº °ÍÀ¸·Î ³ªÅ¸³­µ¥ ¹ÝÇØ aacC2 À¯ÀüÀÚ´Â ÇÏõÀÇ ÇÏ·ùÀÎ Á¦5ÁöÁ¡¿¡¼­ °¡Àå ¸¹ÀÌ ÃâÇöÇÏ¿´´Ù. ÀÌ·¯ÇÑ °á°ú´Â Ç×»ý¹°Áú ÀúÇ×¼º À¯ÀüÀÚ°¡ ¼öÁúÀÇ ¿À¿°¿øÀ» ¾Ë ¼ö Àִ ǥÁö·Î »ç¿ëµÉ ¼ö ÀÖ´Â °¡´É¼ºÀ» ½Ã»çÇÑ´Ù.
The occurrence of tetQ and aacC2 genes encoding tetracycline and gentamicin resistance determinant, respectively, was assessed in total bacterial community DNA isolated from Dongchon stream of Sunchon area. To examine the resistance potential of bacteria that were not cultured, total DNA from 1 liter of stream water was extracted by freeze-thaw method. The PCR technique was employed to determine the abundance of the target genes. The highest frequency of tetQ gene was obtained from site 1, located near the animal farms area, whereas the incidence of aacC2 was highest in site 5, the downstream area. These results showed that the occurrence of antibiotic resistance gene may be used as a convenient marker of water quality related to source.
 
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ÀúÇ×¼ºÀ¯ÀüÀÚ;Ç×»ý¹°Áú;aacC2;Antibiotics resistance;PCR;tetQ;
 
The Korean Journal of Ecology / v.22, no.5, 1999³â, pp.305-309
Çѱ¹»ýÅÂÇÐȸ
ISSN : 1225-0317
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199911922413251)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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