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Çѱ¹»ýÅÂÇÐȸ / v.16, no.4, 1993³â, pp.515-526
¾Æ±î½Ã³ª¹«(Robinia pseudo-acacia)Á¾ÀÚ ´Ü¹éÁúÀÇ Àü±â ¿µµ¿ º¯ÀÌ
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Çѹݵµ ³²ºÎ Áö¿ª¿¡ ºÐÆ÷ÇÏ´Â ¾Æ±î½Ã³ª¹«(Robinia pseudo-acacia L.)Á¾ÀÚÀÇ »ýÅÂÇÐÀû ÇüÁúº¯À̸¦ ÃßÀûÇϱâ À§ÇÏ¿©, ºÏÀ§34$^{circ}$18'~38$^{circ}$36'»çÀÌ¿¡ À§Ä¡ÇÑ 15°³ Áö¿ª(´ëÁø, ¼ÓÃÊ, °­¸ª, ¼ö¶ô»ê, ȫõ, ±¤¸ª, ³²Çѻ꼺, ÃæÁÖ, ¿¹»ê, ¾Èµ¿, ÀüÁÖ, ´Þ¼º, â¿ø, ¸ñÆ÷, ¿Ïµµ)À» ¼±Á¤ÇÏ¿© äÁ¾ÇÑ Á¾ÀÚ¸¦ Àç·á·Î, ´Ü¹éÁú ÆÐÅÏ ¹× Á¤·® ºÐ¼®À» ½Ç½ÃÇÏ¿´´Ù. SDS-PAGE¿¡ ÀÇÇÑ ´Ü¹éÁú ÆÐÅÏ ºÐ¼®°á°ú, Àü Áö¿ªµ¥ °ÉÃÄ ÃÑ 35°³ÀÇ ¹êµå°¡ ºÐ¸®µÇ¾úÀ¸¸ç ÃÖ°í ºÐÀÚ·®Àº 142,232 daltons, ÃÖÀú ºÐÀÚ·®Àº 17,258 daltons¿´´Ù. ºÐ¸®µÈ ´Ü¹éÁú ¹êµå ¼ö¸¦ Áö¿ªº°·Î ºñ±³ÇÑ °á°ú, ´ëÁø, ¼ÓÃʰ¡ 32°³·Î °¡Àå ¸¹Àº ¹Ý¸é, ȫõ°ú ´Þ¼ºÀº 23°³·Î °¡Àå Àû¾úÀ¸¸ç, ´ëü·Î °íÀ§µµ·Î °¥¼ö·Ï Áõ°¡ÇÏ´Â °æÇâÀ» ³ªÅ¸³Â´Ù. °¢ ´Ü¹éÁú ¹êµåÀÇ À¯¹«¿¡ µû¶ó Áö¿ª¿¡ µû¸¥ ´Ü¹éÁú ÆÐÅÏÀÇ Â÷À̸¦ ºñ±³ÇØ º» °á°ú, ÁߺϺΠµ¿ÇؾÈÇü(´ëÁø, ¼ÓÃÊ, °­¸ª)°ú ÁߺÎÇü(¼ö¶ô»ê, ȫõ, ±¤¸ª, ³²Çѻ꼺, ÃæÁÖ) ¹× ³²ºÎÇü(¿¡»ê, ¾Èµ¿, ÀüÁÖ, ´Þ¼º, â¿ø, ¸ñÆ÷, ¿Ïµµ)µî 3°¡Áö Áö¿ª À¯ÇüÀÌ ±¸ºÐµÇ¾ú´Ù. ´Ü¹éÁú ÆÐÅÏÀÇ Áö¿ª°£ À¯»çµµ Áö¼ö(Jaccard °è¼ö)¸¦ ±Ù°Å·Î UPGMAdp ÀÇÇÑ cluster analysis °á°ú, ÁߺϺΠµîÇØ¾ÈÇü(¼ÓÃÊ, °­¸ª), ÁߺϺΠIÇü(¼ö¶ô»ê, ȫõ), ÁߺϺΠIIÇü(³²Çѻ꼺, ÃæÁÖ, ´ëÁø), ÁߺϺÎIIIÇü(±¤¸ª), Áß³²ºÎÇü(¿¹»ê, ´Þ¼º, ÀüÁÖ) ¹× ³²ºÎÇü(¾Èµ¿, â¿ø, ¸ñÆ÷, ¿Ïµµ)µî 6°³ À¯ÇüÀÌ ½Äº°µÇ¾ú´Ù. ºÐ¸®µÈ ´Ü¹éÁú ¹êµå °¡¿îµ¥, ¸ðµç Áö¿ª¼¼¾î No.12¹êµå(78,162daltons)°¡ °¡Àå ³ôÀº ¿°»ö °­µµ¸¦ ³ªÅ¸³Â°í, No.11.12.13.°úNo.23~28 ¹êµå ¿ª½Ã ºñ±³Àû ³ôÀº ¿°»ö°­µµ¸¦ º¸¿´´Ù. ÀüüÀûÀ¸·Î ÃÑ ´Ü¹éÁú ÇÔ·®ÀÌ ³ôÀº â¿ø, ¸ñÆ÷, ¿Ïµµ µî ³²ºÎÇü Áö¿ª¿¡¼­ ƯÈ÷ ³ôÀº ¿°»ö °­µµ¸¦ ³ªÅ¸³Â´Ù. ÃÑ ´Ü¹éÁú ÇÔ·®Àº °¢ Áö¿ªº°·Î ÃÖÀú9.68mg/g(¼ö¶ô»ê)¿¡¼­ ÃÖ°í17.30mg/g(ÀüÁÖ)¿¡ À̸£´Â ´Ù¾çÇÑ ¼öÄ¡¸¦ ³ªÅ¸³ÂÀ¸¸ç, ´ëü·Î ÀúÀ§µµ·Î °¥¼ö·Ï Áõ°¡ÇÏ´Â °æÇâÀ» º¸¿´´Ù.
In order to study the ecotypic variation of Rohinia pseudo-acacia L. distributed in southern area of Korean peninsula, 15 local populations(Daejin, Sokcho, Kangneung, Mt. Surak, Hongcheon, Kwangneung, Namhansanseong, Chungju, Yesan, Andong, Jeonju, Dalseong, Changweon, Mokpo and Wando), located from $34^{circ}18'N;to;38^{circ}36'N$, were selected based on the latitudes and geographical distances. Seeds of these populations were collected and protein contents of seeds and their band patterns were investigated. The seed proteins of all populations were electrophoresed on SDS-polyacrylamide gel. Total number of protein bands were 35, whose molecular weights ranged from 17, 258 daltons to 142, 232 daltons. The number of bands of seed proteins was 23 in Dalseong and Hongcheon and was 32 in Daejin and Sokcho, showing an increasing tendency in the number of bands as the latitude goes high. The local populations were classified into 3 local types based on protein analysis: the middle north east coastal type(Daejin, Sokcho. Kangneung), the central type (Mt. Surak, Hongcheon, Kwangneung, Namhansanseong, Chungju) and the southern type(Yesan, Andong, Jeonju, Dalseong, Changweon, Mokpo, Wando). According to the results of cluster analysis by UPGMA based on the similarity index(c0efficient of Jaccard) of the patterns, 3 local types were subdivided further into 6 types: the middle north east coastal type(Sokcho, Kangneung), the north central type I (Mt. Surak, Hongcheon), the north central type II (Narnhansanseong, Chungju, Daejin), the north central type III (Kwangneung), the south central type (Yesan, Dalseong, Jeonju) and the southern type(Andong, Changweon, Mokpo, Dalseong, Wando). The No. 12 band of the separated seed proteins showed the highest colored density in the preparations from all the populations. The No. 11~13 and No. 23~28 bands also showed high densities. As a whole, southern type populations (Changweon, Mokpo, Wando) showed high protein contents and high colored density. Total protein contents of the seeds in each population were variable from 9. 68mg / g (Mt. Surak) to 17.30mg/g (Jeonju), showing an increasing trends toward low latitudes.
 
Ű¿öµå
Robinia pseudo-acacia;Electrophoretic variation;Seed proteins;Ecotypic variation;Protein band patterns;
 
The Korean Journal of Ecology / v.16, no.4, 1993³â, pp.515-526
Çѱ¹»ýÅÂÇÐȸ
ISSN : 1225-0317
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199311919965959)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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