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Çѱ¹»ýÅÂÇÐȸ / v.24, no.1, 2001³â, pp.35-43
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ÃѾ˰íµÕ¿¡¼ Ä«µå¹Å°ú ¾Æ¿¬ÀÇ ÃàÀû°ú Á¦°Å
( Accumulation and Elimination of Cadmium and Zinc in Littorina brevicula ) |
| ÇѼöÁ¤;ÀÌÀμ÷; ÀÌÈ¿©ÀÚ´ëÇб³ »ý¹°°úÇаú;ÀÌÈ¿©ÀÚ´ëÇб³ »ý¹°°úÇаú;
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| Ä«µå¹Å°ú ¾Æ¿¬¿¡ °¢°¢ ±×¸®°í µ¿½Ã¿¡ ³ëÃâÇÑ ÃѾ˰íµÕ(Littorina brevicula)¿¡¼ Á߱ݼÓÀÇ »ýü³» ÃàÀû, Á¦°Å ¹× ¼¼Æ÷³» ºÐÆ÷ ¾ç»óÀ» Á¶»çÇÏ¿´´Ù. ÃѾ˰íµÕÀ» Ä«µå¹Å 400 $mu extrm{g}$/L ¶Ç´Â ¾Æ¿¬ 3000 $mu extrm{g}$/L¿¡ °¢°¢ 90Àϰ£ ³ëÃâÇÏ¿´À» °æ¿ì, °¢ Á߱ݼÓÀÇ ÃàÀû·®Àº ³ëÃâ±â°£¿¡ µû¶ó Áõ°¡ÇÏ¿´À¸¸ç, 70ÀÏ ÀÌÈÄ¿¡´Â ´õ ÀÌ»ó ÃàÀû·®ÀÌ Áõ°¡ÇÏÁö ¾Ê¾Ò´Ù. Ä«µå¹Å°ú ¾Æ¿¬À» µ¿½Ã¿¡ ³ëÃâÇÏ¿´À» °æ¿ì¿¡´Â °¢°¢ÀÇ Á߱ݼӿ¡ ³ëÃâÇÏ¿´À» °æ¿ì¿¡ ºñÇØ ¾Æ¿¬ÀÇ ÃàÀû·®Àº Áõ°¡ÇÏ¿´À¸³ª, Ä«µå¹ÅÀÇ ÃàÀû·®Àº °¨¼ÒÇÏ¿´´Ù. ³ëÃâ½ÇÇè¿¡ ÀÌ¾î ¼öÇàÇÑ 42Àϰ£ÀÇ Ã»Àå½ÇÇè °á°ú, Ä«µå¹ÅÀº ü¿Ü·Î Á¦°ÅµÇÁö ¾Ê¾ÒÀ¸³ª, ¾Æ¿¬Àº Á¦°ÅµÇ¾ú´Ù. ƯÈ÷ Ä«µå¹Å°ú ¾Æ¿¬¿¡ µ¿½Ã ³ëÃâÇÑ ÃѾ˰íµÕÀÇ °æ¿ì¿¡ ¾Æ¿¬Àº ´õ ½Å¼ÓÈ÷ Á¦°ÅµÇ¾ú´Ù. ÃѾ˰íµÕÀ» Ä«µå¹Å°ú ¾Æ¿¬¿¡ °¢°¢ 70ÀÏ µ¿¾È ³ëÃâÇÑ ÈÄ, ü³»·Î Èí¼öµÈ Ä«µå¹ÅÀÇ ¾à 60%°¡ soluble fraction¿¡ ºÐÆ÷Çϰí ÀÖ¾úÀ¸¸ç, ¾Æ¿¬ÀÇ 75%´Â insoluble fraction¿¡ ºÐÆ÷Çϰí ÀÖ¾ú´Ù. ÀÌ·¯ÇÑ °æÇâÀº Ä«µå¹Å°ú ¾Æ¿¬ÀÇ µ¿½Ã ³ëÃâ½Ã¿¡µµ À¯»çÇÏ°Ô ³ªÅ¸³µ´Ù. Ä«µå¹Å°ú ¾Æ¿¬Àº soluble part³»ÀÇ ¸®°£µå(ligand) ¿ÍÀÇ °áÇÕ ¾ç»ó¿¡¼µµ Â÷À̸¦ ³ªÅ¸³Â´Âµ¥, Ä«µå¹ÅÀº 90% ÀÌ»óÀÌ ¾à 6.5 kDaÅ©±âÀÇ MBP-1(Metal-Binding Protein-1)°ú °áÇÕÇϰí ÀÖ¾úÀ¸³ª, ¾Æ¿¬Àº HMW(High molecular weight fraction, >60 kDa), MBP-1, MBP-2, LMW(Low molecular weight fraction <1 kDa)¿¡ °í·ç ºÐÆ÷ÇÏ´Â °ÍÀ¸·Î ³ªÅ¸³µ´Ù. |
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| Accumulation, elimination and subcellular distribution of heavy metals in Littorina brevicula exposed to cadmium and zinc separately and concurrently were investigated. When the winkles had been exposed to 400 §¶/L CdCl©ü and 3000 §¶/l ZnSO©þ separately for 90 days, each of the metal body burden in the whole sofl parts increased in proportion to time of exposure until 70 days. But it didn't increase after 70 days. But when the winkles had been exposed to cadmium and zinc simultaneously, cadmium body burden decreased but zinc body burden increased as compared to the winkles exposed to each of the metal. We also found that cadmium accumulated in the winkles was not depurated for 42 days, but zinc accumulated in them was depurated. Especially, zinc was depurated faster when they had been exposed to mixture of cadmium and zinc. After the winkles had been exposed to cadmium and zinc separately for 70 days, about 60% cadmium of the total body burden was associated with the soluble fraction, while about 75% zinc of the total body burden was associated with insoluble fraction. And these trends of metal partitioning did not alter when the winkles had been exposed to metal mixture. After the soluble fraction applied to gel-filtration chromatography column, the distribution patterns of cadmium and zinc associated with proteins or ligands were different each other. Most of cadmium (£¾90%) in the soluble fraction was bound to MBP-1 (Metal-binding protein-1), about 6.5 kDa), while zinc was distributed evenly to HMW (High molecular weight fraction, >60 kDa), MBP-1, MBP-2 (about 5 kDa), LMW (Low molecular weight fraction, <1 kDa). |
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| ¾Æ¿¬;Á¦°Å;ÃѾ˰íµÕ;ÃàÀû;Ä«µå¹Å;Accumulation;Cadmium;Elimination;Littorina brevicula;Zinc; |
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The Korean Journal of Ecology / v.24, no.1, 2001³â, pp.35-43
Çѱ¹»ýÅÂÇÐȸ
ISSN : 1225-0317
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200111921336542)
¾ð¾î : Çѱ¹¾î |
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| ³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø |
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