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한국생태학회 / v.26, no.5, 2003년, pp.263-266 
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( Purification and Characterization of PC-Like Cadmium-Binding Peptide from Root of Rumex crispus ) |
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| 초 록 |
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| This research investigated the process of removing cadmium and tested the detoxification mechanism of the cadmium-binding peptide (Cd-BP) from Rumex crispus. Phytochelatin-like cadmium-binding peptide (PC-Cd-BP) of Rumex crispus was purified and identified. Rumex crispus was exposed to 4.3 mg Cd/L for seven days. Heat-treated supernatant fraction taken by root tissues showed traces of PC-Cd-BP An analysis of the material through Gel-filteration chromatography on the Sephadex G-75 column showed two symmetrical Cd-BP peaks. The major peak with the smaller molecular weight was further purified by $C_{18}$ reverse-phase HPLC to produce apparent homogeneity. The amino acid composition of Cd-BP from Rumex crispus included cysteine (22.6%), glutamate and glutamate acid (20%), and glycine (12%). It was similar the amino acid composition of most PC. The molecular weight of the purified peptide was determined at 568-706 Da by MALDI-TOF MS. Therefore, the Cd-BP of Rumex crispus was PC-Cd-BP consisting of isopeptides. |
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| 키워드 |
| Amino acid composition;MALDI-TOF MS;Phytochelatin-like cadmium-binding peptide (PC-Cd-BP);Rumex crispus; |
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The Korean Journal of Ecology / v.26, no.5, 2003년, pp.263-266
한국생태학회
ISSN : 1225-0317
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200311922043635)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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