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Çѱ¹»ýÅÂÇÐȸ / v.26, no.6, 2003³â, pp.307-312
16S rDNA¿°±â¼­¿­¿¡ ÀÇÇÑ ºÒ°¡»ç¸®(Asterias amurensis) Àå³»¿¡¼­ ºÐ¸®µÈ Á¾¼Ó¿µ¾ç¼¼±Õ ±ºÁýÀÇ ´Ù¾ç¼º
( The Diversity of Heterotrophic Bacteria Isolated from Intestine of Starfish(Asterias amurensis) by Analysis of 16S rDNA Sequence )
ÃÖ°­±¹;ÀÌ¿ÀÇü;À̰ÇÇü; Ãæ³²´ëÇб³ ÀÚ¿¬°úÇдëÇÐ ¹Ì»ý¹°Çаú;¸ñÆ÷´ëÇб³ ÀÚ¿¬°úÇдëÇÐ ºÐÀÚÀ¯ÀûÇÐ;±º»ê´ëÇб³ ÀÚ¿¬°úÇдëÇÐ °úÇбâ¼úÇкÎ;
 
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º» ¿¬±¸´Â 2000³â 7¿ù¿¡ Àü³² ÀåÈﱺ¿¡¼­ äÁýÇÑ ºÒ°¡»ç¸®ÀÇ Àå³»¿¡ Á¸ÀçÇÏ´Â Á¾¼Ó¿µ¾ç¼¼±ÕÀÇ ´Ù¾ç¼º¿¡ ´ëÇØ¼­ ¾Ë¾Æº¸¾Ò´Ù. ºÒ°¡»ç¸® Àå³»¿¡ Á¸ÀçÇÏ´Â ±Õü¼ö¸¦ ÃøÁ¤ÇÏ¿´À¸¸ç, ¼ø¼ö ºÐ¸®µÈ ±ÕÁÖ¸¦ ´ë»óÀ¸·Î 16S rDNA ÁõÆø±â¹ýÀ» ÀÌ¿ëÇÏ¿© ¼¼±ÕÀÇ ´Ù¾ç¼ºÀ» Á¶»çÇÏ¿´´Ù. ºÒ°¡»ç¸® Àå³»¿¡ ºÐÆ÷ÇÏ´Â Á¾¼Ó¿µ¾ç¼¼±ÕÀÇ ±Õü¼ö´Â 8.65${pm}$0.65${ imes}10^3;dfu;g^{-1}$À̾ú´Ù. 29 ±ÕÁÖÀÇ ¼¼±ÕÀÌ ¼ø¼ö ºÐ¸®µÇ¾úÀ¸¸ç, ±× Áß ±×¶÷¾ç¼º ¼¼±ÕÀº ºÐ¸®µÈ ±ÕÁÖÀÇ 59% (17±ÕÁÖ)¸¦ Â÷ÁöÇÏ¿´´Ù. ºÒ°¡»ç¸® Àå³»¿¡¼­ ºÐ¸®µÈ ±ÕÁÖ´Â Bacillus¼Ó, Microbacterium ¼Ó, ±×¸®°í Marinobacter ¼Ó µîÀÌ ¿ìÁ¡À̾úÀ¸¸ç, À̿ܿ¡µµ Staphylococcus ¼Ó, Psychrobacter ¼Ó, Paracoccus ¼Ó, Erythrobacter ¼Ó, Zoogloea ¼Ó, Kocuria ¼Ó°ú Arthrobacter ¼Ó µîÀÌ Æ÷ÇԵǾú´Ù. ºÐ¸®µÈ ±ÕÁÖ °¡¿îµ¥ Bacillus ¼Ó¿¡ ¼ÓÇÏ´Â 8±ÕÁÖ Áß 3±ÕÁÖ´Â type strain°ú 97% ÀÌ»óÀÇ À¯»çµµ¸¦ º¸ÀÎ ¹Ý¸é, 5 ±ÕÁÖ´Â À¯»çµµ°¡ 90%·Î ºñ±³Àû ³·Àº À¯»çµµ¸¦ º¸¿© ÇöÀç±îÁö ¾Ë·ÁÁöÁö ¾ÊÀº ½ÅÁ¾ÀÏ °¡´É¼ºÀÌ ³ô´Ù°í ÇϰڴÙ.
To study the diversity of heterotrophic bacteria isolated from intestine of starfish, Asterias amurensis, we collected starfishes from the coastal area near Jangheung-Gun, Jeollanam-Do, Korea during July, 2000. Population density and bacterial diversity in the intestine of starfish were measured. The results were as follows; The population densities of heterotrophic bacteria in the intestine of starfish were 8.65${pm}$0.65${ imes}10^3;dfu;g^{-1}$. Gram positive bacteria occupied 59% among 29 isolates. The community structure of dominant heterotrophic bacteria in the intestine of starfish consisted of Bacillaceae in the low G+C gram positive bacteria subphylum, Microbacteriaceae in the high G+C gram positive bacteria subphylum, and Alteromonadaceae in ${gamma}$-Proteobacteria subphylum. Among eight strains of Bacillus spp., three strains showed more than 97% identity, but five strains showed about 90% identity with type strain on the basis of partial 16S rDNA sequence.
 
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°èÅë¼ö;±Õü¼ö;ºÒ°¡»ç¸®;¿ìÁ¡¼Ó;16S rDNA;Dominant genus;Phylogenetic tree;Population density;Starfish (Asterias amurensis);
 
The Korean Journal of Ecology / v.26, no.6, 2003³â, pp.307-312
Çѱ¹»ýÅÂÇÐȸ
ISSN : 1225-0317
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200311922123317)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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