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Çѱ¹»ýÅÂÇÐȸ / v.28, no.6, 2005³â, pp.375-382
¹ÌÅäÄܵ帮¾Æ ND1/tRNA À¯ÀüÀÚ ¼­¿­ ºñ±³¸¦ ÅëÇÑ ±¹³» ¼­½Ä Ȳ¼Ò°³±¸¸®ÀÇ À¯ÀüÀû ´Ù¾ç¼º Á¶»ç
( Genetic Diversity of Rana catesbeiana in Korea based on Mitochondrial ND1/tRNA Sequence Analysis )
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1970³â ´ë ½Ä¿ëÀ» À§ÇÑ ¾ç½ÄÀ» ¸ñÀûÀ¸·Î ÀϺ»¿¡¼­ µµÀÔµÈ È²¼Ò°³±¸¸®´Â 1990³â´ë ÃʹݱîÁö ±¹³» ÇÏõ°ú È£¼Ò »ýŰ迡 Å« ÇÇÇØ¸¦ ÁÖ¾úÀ¸³ª ÃÖ±Ù ±Þ¼ÓÈ÷ ±× °³Ã¼¼ö°¡ ÁÙ¾îµç °ÍÀ¸·Î ÃßÁ¤µÈ´Ù. À̹ø ¿¬±¸¿¡¼­´Â Ȳ¼Ò°³±¸¸®ÀÇ °³Ã¼±º µ¿Å¿¡ ¹ÌÄ¡´Â À¯ÀüÀû ¿äÀÎÀ» Á¶»çÇϱâ À§ÇÏ¿© ±¹³»¿¡ ¼­½ÄÇϴ Ȳ¼Ò°³±¸¸®ÀÇ À¯ÀüÀÚ ºÐ¼®À» ½Ç½ÃÇÏ¿´´Ù. Ȳ¼Ò°³±¸¸® ÃâÇöÀÌ ¸¹Àº Àü¶ó³²µµ 5°³ Áö¿ª¿¡¼­ Áö¿ªº°·Î äÁýÇÑ °³Ã¼ÀÇ ¹ÌÅäÄܵ帮¾Æ ND1/tRNA À¯ÀüÀÚ 1,215 bpÀÇ ¿°±â ¼­¿­À» °áÁ¤Çϰí À̸¦ ±âÁ¸¿¡ ¹ßÇ¥µÈ Ȳ¼Ò°³±¸¸®ÀÇ ¿°±â¼­¿­°ú ºñ±³, ºÐ¼®ÇÑ °á°ú ¸ðµç °³Ã¼ÀÇ 1°³ À§Ä¡¿¡¼­ ¿°±â º¯È­°¡ ¹ß°ßµÇ¾ú´Ù. ¶ÇÇÑ Á¶»çÇÑ °³Ã¼ ÀϺο¡¼­ À¯ÀüÀÚ ¿°±â ¼­¿­ÀÇ 6°³ À§Ä¡¿¡¼­ÀÇ º¯À̰¡ ¹ß°ßµÇ¾úÀ¸³ª ÀÌ´Â 1,215bp¿¡¼­ ±ØÈ÷ ÀϺκÐÀÌ º¯È­µÈ °ÍÀ¸·Î À¯ÀüÀû º¯À̰¡ ÀÏ¾î ³µ´Ù°í ÆÇ´ÜÇÏ±â ¾î·Æ´Ù. Kimura-2-parameter distance ºÐ¼®¿¡¼­ ±¹³» ¼­½Ä Ȳ¼Ò°³±¸¸®´Â $98.7%sim100%$ÀÇ ³ôÀº À¯»çµµ¸¦ º¸¿© Á¾³» À¯ÀüÀû °Å¸®ÀÇ Â÷À̰¡ °ÅÀÇ ¾ø¾úÀ¸¸ç À¯ÀüÀûÀ¸·Î À¯»çÇÑ °³Ã¼µéÀÌ cluster¸¦ Çü¼ºÇÏ´Â Neighbor-Joining ºÐ¼® °á°ú¿¡¼­ ¿ø»êÁö Ȳ¼Ò°³±¸¸®°¡ ±¹³» ¼­½Ä Ȳ¼Ò°³±¸¸®ÀÇ ÀÏºÎ¿Í ÇÔ²² °°Àº cluster¿¡ ¼ÓÇϹǷΠ¿ø»êÁö¿Í ±¹³» ¼­½Ä Ȳ¼Ò°³±¸¸®´Â À¯ÀüÀû Â÷À̰¡ ÀûÀº °ÍÀ» ¾Ë ¼ö ÀÖ¾ú´Ù. ÀÌ·¯ÇÑ °á°úµé·ÎºÎÅÍ ±¹³»¿¡ ¼­½ÄÇϴ Ȳ¼Ò°³±¸¸®´Â Á¾³» À¯ÀüÀû À¯»çµµ°¡ ¸Å¿ì ³ôÀ¸¸ç ±¹³»¿¡ µµÀÔµÈ ÈÄ Áö¿ª ƯÀÌÀûÀ¸·Î À¯ÀüÀû ºÐÈ­°¡ °ÅÀÇ ÀϾÁö ¾ÊÀº °ÍÀ» ¾Ë ¼ö ÀÖ´Ù.
The American bullfrog, Rana catesbeiana was imported from Japan for farming for the human consumption in 1970's and introduced populations were a great threat to native habitats in the pond and lake ecosystem. However, it is thought that the population of bullfrog has rapidly declined for past years in Korea. In this study, we investigated the intra-genetic diversity of R. catesbeiana habitated in Korea. The nucleotide sequences of 1,215bp mitochondrial ND1/tRNA region in bullfrogs sampled from 5 sites in Jeollanamdo were analyzed and compared to the original sequence of R. catesbeiara reported in Genbank. The nucleotide similarity between Korean and North American bullfrog was ranged from 98.7% to 100% based on kimura-2-parameter distance. In addition, bullfrogs analyzed in this study were clustered into two groups with one including Jangheung and the other including Gwangju populations in the neighbor-joining tree. North American R. catesbeiana was grouped in Jangheung cluster, indicating that there is the very low genetic difference between Korean and North American populations. The maximum parsimony tree in which North American R. catesbeiana was set as an outgroup suggests that Jangheung group represents the introduced population to Korea. Taken together, the results indicate that the population of R. catesbeiana in Korea has not segregated geographically yet, after the introduction.
 
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Genetic similarity;Mitochondrial ND1/tRNA;Rana catesbeiana in Korea;
 
The Korean Journal of Ecology / v.28, no.6, 2005³â, pp.375-382
Çѱ¹»ýÅÂÇÐȸ
ISSN : 1225-0317
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200509905770963)
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