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Çѱ¹»ýÅÂÇÐȸ / v.29, no.6, 2006³â, pp.551-558
¼Ò³ª¹«(Pinus densiflora) »ýÀ°Åä¾çÀÇ ¹Ì»ý¹° ±ºÁý¿¡ ¹ÌÄ¡´Â ³³°ú CO2ÀÇ ¿µÇâ
( Effects of Pb and CO2 on Soil Microbial Community Associated with Pinus densiflora-Lab )
È«¼±È­;±è¼ºÇö;°­È£Á¤;·ùÈñ¿í;ÀÌ»óµ·;ÀÌÀμ÷;Á¶°æ¼÷; ÀÌÈ­¿©ÀÚ´ëÇб³ ȯ°æ°øÇаú;ÀÌÈ­¿©ÀÚ´ëÇб³ »ý¸í°úÇаú;ÀÌÈ­¿©ÀÚ´ëÇб³ ȯ°æ°øÇаú;¼þ½Ç´ëÇб³ ȯ°æÈ­ÇаøÇаú;ÀÌÈ­¿©ÀÚ´ëÇб³ ȯ°æ°øÇаú;ÀÌÈ­¿©ÀÚ´ëÇб³ »ý¸í°úÇаú;ÀÌÈ­¿©ÀÚ´ëÇб³ ȯ°æ°øÇаú;
 
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¿ì¸®³ª¶ó »ê¸²ÀÇ ´ëÇ¥Àû ÀΠħ¿±¼öÀÎ ¼Ò³ª¹«(Pinus densiflora) »ýÀ° Åä¾çÀÇ ¹Ì»ý¹° ±ºÁý¿¡ ¹ÌÄ¡´Â $CO_2$¿Í ³³ÀÇ ¿µÇâÀ» ÆÄ¾ÇÇϱâ À§ÇØ, ±ºÁý ¼öÁØ ±âÁú À̿뵵¸¦ Æò°¡ÇÏ´Â CLPP (community level rhysiological profiles) ¹æ¹ý°ú 165 rDNA PCR-denaturing gradient gel electrophoresis (DGGE) ¹æ¹ýÀ» Ȱ¿ëÇÏ¿© Åä¾ç ¹Ì»ý¹°±ºÁý Ư¼ºÀ» Á¶»çÇÏ¿´´Ù. ³³ ¿À¿° Åä¾ç(500 mg/kg-soil)°ú ºñ¿À¿° Åä¾ç¿¡ 2³â»ý ¼Ò³ª¹«¸¦ ½ÄÀçÇÑ ÈÄ, $CO_2$ ³óµµ¸¦ 380 ppmv ȤÀº 760 ppmvÀ¸·Î Á¶ÀýÇÑ ¹è¾ç±â¿¡¼­ 3°³¿ù°£ »ýÀ°½ÃŲ ÈÄ 6Á¾·ùÀÇ Åä¾Ó ½Ã·áÀÇ ¹Ì»ý¹° ±ºÁýÀ» ºñ±³ ºÐ¼®ÇÏ¿´´Ù. 3°³¿ù ÈÄ ºñ¿À¿° Åä¾ç(CA-3M vs EA-3M)ÀÇ ±âÁú À̿뵵´Â $CO_2$¿¡ ÀÇÇØ Å©°Ô ¿µÇâÀ» ¹ÞÁö ¾Ê¾ÒÀ¸³ª, ³³¿À¿° Åä¾ç(OB-3M vs EB-3M)ÀÇ °æ¿ì¿¡´Â $CO_2$¸¦ 760 ppmv·Î ³ôÀÎ Åä¾ç ½Ã·á(EB-3M)ÀÇ ±âÁú À̿뵵°¡ ³ô¾Ò´Ù. °¢ ½Ã·á°£ÀÇ ±âÁú À̿뵵¸¦ ÀÌ¿ëÇÏ¿© PCA¸¦ ¼öÇàÇÑ °á°ú, °¢ Åä¾ç½Ã·áÀÇ ¹Ì»ý¹° ±ºÁýÀº ³³ÀÇ Á¸Àç À¯¹«¿¡ µû¶ó ±×·ìÈ­ µÇ¾ú´Ù. ºñ¿À¿° Åä¾ç(CA-3M vs EA-3M))»çÀÌÀÇ DGGE fingerprint À¯»ç¼ºÀº 56.3%, ³³ ¿À¿° Åä¾ç(CB-3M vs EB-3M) »çÀÌÀÇ DGGE fingerprint À¯»ç¼ºÀº 71.4%¿´´Ù. µ¿ÀÏ $CO_2$ ³óµµ ½Ã·áÀÎ CA-3M°ú CB-3M»çÀÌÀÇ À¯»ç¼ºÀº 53.3%, EA-3M°ú EB-3M»çÀÌÀÇ À¯»ç¼ºÀº 35.8%À̾ú´Ù. ÀÌ·¯ÇÑ °á°ú´Â ¼Ò³ª¹«¸¦ ½ÄÀçÇÑ Åä¾çÀÇ ¼¼±Õ ±ºÁý ±¸Á¶´Â $CO_2$ ³óµµº¸´Ù´Â ³³ ¿À¿° ¿©ºÎ¿¡ ÀÇÇØ ´õ ¹Î°¨ÇÏ°Ô Æ¯¼ºÈ­µÊÀ» ÀǹÌÇÑ´Ù.
Effects of Pb and $CO_2$ on soil microbial community associated with Pinus densiflora were investigated using community level physiological profiles (CLPP) and 16S rDNA PCR-denaturing gradient gel electrophoresis (DGGE) methods. Two-years pine trees were planted in Pb-contaminated soils and uncontaminated soils, and cultivated for 3 months in the growth chamber where $CO_2$ concentration was controlled at 380 or 760 ppmv. The structure of microbial community was analyzed in 6 kinds of soil samples (CA-0M : $CO_2$ 380 ppmv + Pb 0 mg/kg + initial, CB-0M : $CO_2$ 380 ppmv + Pb 500 mg/kg + initial, CA-3M : $CO_2$ 380 ppmv + Pb 0 mg/kg + after 3 months, CB-3M : $CO_2$ 380 ppmv + Pb 500 mglkg + after 3 months, EA-3M : $CO_2$ 760 ppmv + Pb 0 mg/kg + after 3 months, EB-3M : $CO_2$ 760 ppmv + Pb 500 mg/kg + after 3 months). After 3 months, the substrate utilization in the uncontaminated soil samples (CA-3M vs EA-3M) was not significantly influenced by $CO_2$ concentrations. However, the substrate utilization in the Pb-contaminated soil samples (CB-3M vs EB-3M) was enhanced by the elevated $CO_2$ concentrations. The results of principal component analysis based on substrate utilization activities showed that the structure of microbial community structure in each soil sample was grouped by Pb-contamination. The similarities of DGGE fingerprints were 56.3 % between the uncontaminated soil samples (CA-3M vs EA-3M), and 71.4% between the Pb-contaminated soil samples (CB-3M vs. EB-3M). The similarities between the soil samples under $CO_2$ 380 ppmv (CA-3M vs CB-3M) and $CO_2$, 760 ppmv (EA-3M vs EB-3M) were 53.3% and 35.8%, respectively. These results suggested that the structure of microbial community associated with Pinus densiflora were sensitively specialized by Pb-contamination rather than $CO_2$ concentration.
 
Ű¿öµå
$CO_2$;Forest soil;Heavy metal;Soil microbial community;
 
Journal of Ecology and Field Biology / v.29, no.6, 2006³â, pp.551-558
Çѱ¹»ýÅÂÇÐȸ
ISSN : 1975-020X
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200606142024683)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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