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한국식물학회 / v.52, no.2, 2009년, pp.147-153 
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( Salt Tolerance is Conferred in Arabidopsis by Overexpression of the Vacuolar Na(+)/H(+) Antiporter Gene SsNHX2, an Alternative Splicing Variant of SsNHX1, from Suaeda salsa ) |
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| 초 록 |
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| Vacuolar $Na^+/H^+$ antiporters catalyze the exchange of $Na^+;for;H^+$ across vacuolar membranes and compartmentalize $Na^+$ into vacuoles. They play important roles in cellular pH and $Na^+$ homeostasis. The SsNHX1 gene was previously cloned from a typical euhalophyte, Suaeda salsa. Its cloning revealed another N-terminus truncated transcript, SsNHX2. This potentially alternative splicing variant was truncated from the 49 amino acid residues (aa) of the N terminus of SsNHX1. To compare their degree of salt tolerance, we over-expressed SsNHX1 and SsNHX2 in Arabidopsis. Southern and northern blot analyses showed that both genes had been integrated into that genome and had expressed in several lines. Two types of transgenic plants grew more vigorously than the wild type (WT) under salt stress, but no remarkable differences were found between those SsNHX1 and SsNHX2 transformants. Physiological analyses also supported this phenotype. Both fresh and dry weights of the transgenics as well as their accumulations of $Na^+;and;K^+$ under salinity were much higher than that of WT, but differences were not significant between SsNHX1 and SsNHX2 plants for any of those parameters. These results suggest that SsNHX2 is a functional $Na^+/H^+$ antiporter like SsNHX1 and their levels of salt tolerance are similar |
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| 키워드 |
| Alternative splicing;Overexpression;Salt tolerance;SsNHX1;SsNHX2;Suaeda salsa;Vacuolar$Na^+/H^+$ antiporter; |
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Journal of Plant Biology / v.52, no.2, 2009년, pp.147-153
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200917639073479)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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