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한국식물학회 / v.52, no.1, 2009년, pp.41-48 
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( Flavonoid O-Diglucosyltransferase from Rice: Molecular Cloning and Characterization ) |
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| 초 록 |
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| Among more than 100 rice uridine diphosphate glycosyltransferases (UGTs), OsUGT-3 was selected as a candidate for producing flavonoid O-diglycosyltransferases based on phylogenetic analysis and molecular docking. This gene was functionally expressed in Escherichia coli. Analysis of kaempferol, luteolin, quercetin, and tricin reaction products using liquid chromatography-mass spectrometry revealed that these were diglucosylated. The glucosylation positions of kaempferol, which was the best substrate, were determined to be the 3- and 7-hydroxyl groups. This is the first flavonoid O-diglucosyltransferase described from rice. |
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| 키워드 |
| Flavonoids;Flavonoid O-diglucoside;Glycosyltransferase;Oryza sativa;UGT; |
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Journal of Plant Biology / v.52, no.1, 2009년, pp.41-48
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200915536395638)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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