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한국식물학회 / v.52, no.1, 2009년, pp.65-72 
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( Identification and Functional Analysis of Phosphoproteins Regulated by Auxin in Arabidopsis Roots ) |
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| 초 록 |
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| The phytohormone auxin modulates diverse aspects of plant growth and development. Protein phosphorylation is believed to play a key role in regulating auxin-mediated responses. To determine the phosphoproteins affected by auxin in Arabidopsis, we used phospho-specific antibodies to analyze their profiles on two-dimensional gels, then identified them by mass spectrometry. We found two phosphoproteins, enolase and the beta subunit of succinyl-CoA synthetase (SCS-beta), and noted that their phosphorylation was increased by auxin. To investigate their importance in auxin-mediated processes, we characterized Arabidopsis knockout mutants of the two genes. A homozygous null mutation in the gene for SCS-beta conferred embryo lethality. The enolase knockout mutants showed defects in root development similar to those of auxin-related mutants such as alf3 and xbat32. Therefore, we suggest that enolase is involved in auxin-regulated processes. |
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| 키워드 |
| Arabidopsis;Auxin;Enolase;Phosphorylation;Succinyl-CoA synthetase; |
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Journal of Plant Biology / v.52, no.1, 2009년, pp.65-72
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200915536395644)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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