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한국식물학회 / v.41, no.3, 1998년, pp.193-200 
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( Transformation System of Rice suspension-Cultured Microcolonies by Electroporation ) |
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| 초 록 |
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| For establishing a transformation system of rice (Oryza sativa), after three days of culture embryogenic suspension-cultured cell clusters were enzymatically macerated for 2 hours in electroporation buffer containing 2% cellulase and filtered through 550, 400, 250 and 100 ulcorner stainless mesh. Filtered embryogenic microcolonies of 100-250 ulcorner with pBI121 were electroporated at 400 V/cm for 1.2 ms. Four weeks after the electroporation, stable transformed calli were obtained at a frequency of 72% on the selection medium containing 100 mg/L kanamycin. GUS gene in the genomic DNA among 20 out of 22 putative transformed calli lines were detected by PCR analysis. The expression of GUS gene into the kanamycin-resistance calli was confirmed by spectrophotometric assay and histochemical assay of GUS activity. In ahistochemical study of the transgenic rice regenerants, it was shown that the GUS activity directed by the CaMV 35S promoter was localized mainly in leaf vein and root apex. |
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| 키워드 |
| transformation system;embryogenic microcoloies;electroporation;Oryza sativa;PCR;GUS expression; |
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Journal of Plant Biology / v.41, no.3, 1998년, pp.193-200
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199811920119515)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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