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한국식물학회 / v.30, no.4, 1987년, pp.277-285 
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조직 배양에서의 과당의 능동 수송에 대한 Low Affinity System의 분석
( Analysis of the Low Affinity System of the Uptake of Fructose in Suspension Culture Cells ) |
| 조봉희; 수원대학 생물학과;
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| 초 록 |
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| Undifferentiated suspension cells had the ability to transfer glucose and fructose actively, but the suspension culture cells were unable to transfer saccharide without previously splitting to monosccarides. The uptake of fructose showed the low- and high-affinity system compared to of glucose, which possessed only one saturable uptake system. In this paper, the low affinity system of the uptake of fructose has been studied intensively. Glucose did not inhibit the low affinity system of fructose competitively. The Km value was 47 mM for fructose, 7.4 mM for glucose and Vmax was 69 $mu$mol/h.g fresh weight for fuctose, 9.8 $mu$ mol/h.g fresh weight for glucose. Metabolizer inhibitors, both 50 $mu$M of CCCP and DNP, inhibited 70% of the uptake of the low affinity system of fructose. The proton ions were accompanied by the uptake of fructose. The stoichiometry showed ratio of proton to fructose was 0.17. The mechanism ofthe uptake was fructose-proton-symport. The molecules of fructose accmululated inside 25 times more than outside. Therefore, the low affinity system of fructose was not mere diffusion, but depended on metabolic energy and thus transported actively. The importance of this system was discussed. |
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| 키워드 |
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Journal of Plant Biology / v.30, no.4, 1987년, pp.277-285
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO198711920114239)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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