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한국식물학회 / v.52, no.4, 2009년, pp.298-302 
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( In Planta Measurements of $Na^+$ Using Fluorescent Dye CoroNa Green ) |
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| 초 록 |
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| Fluorescent indicators of $Na^+$ are valuable tools for nondestructive monitoring of its spatial and temporal distribution in plants. We tested whether CoroNa Green fluorescent dye, a newly developed sodium indicator, is suitable for measuring relative concentrations in planta. To determine the ideal conditions for its use, we incubated NaCl-pretreated Arabidopsis thaliana seedlings with different concentrations of CoroNa Green and visualized fluorescence in each organ with a fluorescein isothiocyanate filter. When $50{mu}M$ of dye was applied, fluorescence was distributed more uniformly and intensely in the root tips than in other tissues. Under those conditions, fluorescence gradually increased in the root tips when $Na^+$ was bound to CoroNa Green for concentrations up to 100 mM NaCl. Confocal fluorescence microscopy revealed that when Arabidopsis seedlings were incubated with the same concentration of NaCl, the sos1 mutant had much stronger fluorescence than the wild type. This report is the first to describe the properties of CoroNa Green for measuring $Na^+$ content in intact plants and demonstrates the usefulness of this technique for investigating the mechanism of $Na^+$ homeostasis. |
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| 키워드 |
| Confocal microscope;CoroNa Green;Fluorescein isothiocyanate (FITC);Sodium indicator; |
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Journal of Plant Biology / v.52, no.4, 2009년, pp.298-302
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200931559972029)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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