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한국식물학회 / v.51, no.5, 2008년, pp.359-365 
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( A Sterol Biosynthetic Gene AtCYP51A2 Promoter for Constitutive and Ectopic Expression of a Transgene in Plants ) |
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| 초 록 |
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| Arabidopsis CYP51A2 (AtCYP51A2) mediates the sterol $14{alpha}$-demethylation step in de novo sterol biosynthesis, and is constitutively and highly expressed in all plant tissues (Kim et al., 2005). We exploited the molecular features of its expression and the fundamental role of sterol biosynthesis in cells to develop a plant-derived promoter. Our GUS expression analysis between transgenic Arabidopsis lines for AtCYP51A2::GUS and 35S::GUS revealed that activity of the AtCYP51A2 promoter was comparable to that of the 35S promoter, based on enzymatic activities and protein levels. The AtCYP51A2 promoter was also constitutively active in transgenic tobacco, indicating that 5' regulatory elements could be conserved among CYP51 promoters in dicot plants. A homologue of AtCYP51A2 was identified from rape seed, a crop species closely related to Arabidopsis. Its constitutive tissue expression pattern implies that the application of this AtCYP51A2 promoter is possible for that species. Based on these results, we present a new binary vector system with the plant-derived AtCYP51A2 promoter, which is able to constitutively and ectopically drive a transgene in various dicotyledonous plants. |
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| 키워드 |
| Arabidopsis CYP51A2 promoter;$alpha$-glucuronidase;binary vector;CaMV 35S promoter;constitutive overexpression;sterol biosynthesis; |
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Journal of Plant Biology / v.51, no.5, 2008년, pp.359-365
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200812651530038)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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