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Çѱ¹½Ä¹°ÇÐȸ / v.46, no.2, 2003³â, pp.90-94

( Assessing Competence for Adventitious Shoot Formation in Hypocotyl-Explant Cultures from Catharanthus roseus Cultivars )
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Hypocotyl explants from 22 cultivars of Catharanthus roseus were cultured on various shoot-inducing media to assess their competence for adventitious shoot formation. The Murashige and Skoog (MS) media had been supplemented with 14 $mu$M zeatin and 2.5 $mu$M indole-3-butyric acid (IBA), 4.5 $mu$M BA and 0.5 $mu$M $alpha$-naphthaleneacetic acid (NAA), or 14 $mu$M thidiazuron and 2.5 $mu$M IBA. After eight weeks, the explants from¡®Cooler Raspberry Red¡¯showed the greatest frequency of adventitious shoot formation, followed by¡®Cooler Orchid¡¯and¡®Cooler Treated¡¯. The highest frequency (86.7£¥) for ¡®Cooler Raspberry Red¡¯was attained on the medium enhanced with 14 $mu$M zeatin and 2.5 $mu$M NAA. Excised adventitious shoots were then readily rooted on a half-strength MS basal medium. Afterward, the regenerated plant-lets were transferred to potting soil and grown to maturity in a greenhouse.
 
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Apocynaceae;Catharanthus roseus;indole alkakoids;Madagascar periwinkle;organogenesis;
 
Journal of Plant Biology / v.46, no.2, 2003³â, pp.90-94
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ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200311922037518)
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