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한국식물학회 / v.46, no.3, 2003년, pp.187-198 
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( Changes in Gene Expression during Hairy Root Formation by Agrobac-terium rhizogenes Infection in Ginseng ) |
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| 초 록 |
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| Researchers have widely adopted the hairy root culture system as a means for producing secondary metabolites, including ginsenosides from ginseng. Although bacterial genes are involved, the aspects of plant gene expression are unclear. Using a cDNA microarray approach, we identified genes that are differentially expressed in ginseng hairy roots after Agrobacterium rhizogenes infection. Our goal was to gain an initial understanding of the correlation between hairy root morphology and ginsenoside production. Among the 250 genes analyzed here, 63 (including 14 that are unclassified) were differentially expressed in a hairy root line containing a high level of ginsenosides. Of the genes that had been functionally categorized, 29% and 17% were active in metabolism and stress responses, respectively. Most were primarily associated with ribosomal proteins, thereby functioning in protein synthesis and destination. Their expression was down-regulated in hairy roots having less lateral branching. This phenotype may have resulted from the manipulation of metabolic activities by the translational machinery. |
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| 키워드 |
| Araliaceae;ginsenosides;Panax ginseng;ribosomal protein genes; |
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Journal of Plant Biology / v.46, no.3, 2003년, pp.187-198
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200311922994380)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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