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한국식물학회 / v.46, no.3, 2003년, pp.211-214 
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( A Complete Sequence of the pGA1611 Binary Vector ) |
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| 초 록 |
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| We report the nucleotide sequence of the binary vector pGA1611, which is used for the transformation of foreign DNA into rice. This vector is 13,476 bp long. The 5577-bp T-DNA region consists of a 1987-bp ubiquitine promoter region, 45 bp for the multiple cloning site, a 253-bp nos terminator region, and the 2045-bp CaMV35S-hph-T7 chimaeric gene. The vector backbone (7004 bp) carries oriT, traJ, trfA, tetA, tetR, and oriV. An 892-bp RB region and the 489-bp LB region are also present. The T-DNA possesses 15 unique sites, six of which are at the multiple cloning site. This information will be valuable for cloning foreign DNA and modifying the vector. |
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| 키워드 |
| Binary vector;pGA1611;rice;sequence;transformation; |
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Journal of Plant Biology / v.46, no.3, 2003년, pp.211-214
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200311922994429)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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