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한국식물학회 / v.46, no.4, 2003년, pp.263-270 
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( A Type-I Chalcone Isomerase mRNA Is Highly Expressed in the Root Nodules of Elaeagnus umbellata ) |
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| 초 록 |
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| We have used the hybridization-competition method to isolate EuNOD-CHI from a root nodule cDNA library of Elaeagnus umbellata. This cDNA clone encodes chalcone isomerase (CHI) for a protein of 256 amino-acid residues and a mature molecular mass of 28 kDa. Multiple sequence alignment and phylogenetic analysis have demonstrated that EuNOD-CHI can be classified as Type I. Moreover, northern hybridization shows that the EuNOD-CHI gene is highly expressed in root nodules, with levels increasing during nodule development. The highest level of expression is at 6 to 8 weeks after inoculation, decreasing thereafter. Genomic Southern hybridization also demonstrates that EuNOD-CHI has as many as two copies in the E. umbellata genome. Taken together with the previous results, we propose that the higher expression level of the EuNOD-CHI gene in root nodules is likely associated with this species' defense mechanism against infection by Frankia. |
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| 키워드 |
| cDNA;chalcone isomerase;Elaeagnus umbellata;Frankia;root nodule; |
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Journal of Plant Biology / v.46, no.4, 2003년, pp.263-270
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200311922994560)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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