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한국식물학회 / v.47, no.2, 2004년, pp.111-116 
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( Increased Tolerance to Methyl Viologen by Transgenic Tobacco Plants that Over-express the Cytosolic Glutathione Reductase Gene from Brassica campestris ) |
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| 초 록 |
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| We placed BcGR1, a Chinese cabbage (Brassica campestris var. Pekinensis) gene that encodes cytosolic glutathione reductase (GR), under the control of the cauliflower mosaic virus (CaMV) 35S promoter. This gene was then introduced into tobacco plants via Agrobacterium-mediated transformation. Segregation and northern analyses of the transgenic plants revealed no relationship between the copy number of the introduced gene and levels of BcGR1 transcript. Homozygous lines containing BcGR1 were generated and tested for their acquisition of increased tolerance to oxidative stress. When 10-d-old transgenic tobacco seedlings were treated with 5 to 20 $mu$M methyl viologen (MV), they showed significantly increased tolerance compared with the wild-type seedlings. The most drastic difference was observed at a concentration of 10 $mu$M MV. In addition, when leaf discs were subjected to MV, the transgenic plants were less damaged than the wild types with regard to their electrical conductivity and chlorophyll content. |
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| 키워드 |
| Brassica campestris;glutathione reductase;methyl viologen;oxidative stress;transgenic tobacco plant; |
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Journal of Plant Biology / v.47, no.2, 2004년, pp.111-116
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200411922994922)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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