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한국식물학회 / v.47, no.2, 2004년, pp.149-159 
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( Molecular Cloning and Expression Pattern Analyses of Heat Shock Protein 70 Genes from Nicotiana tabacum ) |
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| 초 록 |
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| We have isolated three genomic clones that code tobacco HSP70s, using a tobacco hsp70 cDNA clone as a probe. NtHSP70-1, NtHSP70-2, and NtHSP70-3 contain full open reading frames of 653, 653, and 648 amino acid residues, respectively. All share three conserved regions, namely the C-terminal substrate binding domain, oligomerization domain, and N-terminal ATPase domain. In a comparison of their amino acid sequences, NtHSP70-1 and NtHSP70-2 were very similar to each other, while NtHSP70-3 showed significant differences, instead being highly homologous to the cytosolic HSP70 members of Arabidopsis thaliana and other plant species. Therefore, NtHSP70-1 and NtHSP70-3 were chosen for further analyses. RNA blot hybridizations showed typical heat shock-responsive expression patterns, although their signal intensities differed significantly. Transcription of NtHSP70-1 was also induced by dehydration stress and hormone treatments, such as BA, GA, and IAA, but that of NtHSP70-3 was not. |
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| 키워드 |
| abiotic stress;expression pattern;genomic clones;heat shock protein 70;hormone response;Nicotiana tabacum; |
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Journal of Plant Biology / v.47, no.2, 2004년, pp.149-159
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200411922995016)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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