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한국식물학회 / v.47, no.3, 2004년, pp.244-253 
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( A Study of Cotyledon Senescence in Cucumber (Cucumis sativus L.) Based on Expressed Sequence Tags and Gene Expression ) |
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| 초 록 |
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| Cucumber cotyledons provide an excellent experimental system in which to investigate developmental changes in gene expression, from the early phase of heterotrophism through phototrophic growth to senescence. A cDNA library was prepared from the final stage of senescing cucumber cotyledons (> 95% yellow) for studying the genes responsible for lipid mobilization during germination and senescence. This library had produced numerous senescence-associated clones in a previous study. Here, a total of 365 cDNA clones and their expression levels were examined via semi-quantitative RT-PCR. Up-regulation of expression was detected for several known and unknown genes. These results were used to investigate the possible functions for senescence-related genes during cotyledon development. |
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| 키워드 |
| cotyledon;cucumber;EST;RT-PCR;senescence; |
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Journal of Plant Biology / v.47, no.3, 2004년, pp.244-253
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200411922995259)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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