|
|
|
한국식물학회 / v.48, no.1, 2005년, pp.39-46 
|
( Constitutive Expression of Two Endochitinases from Root Nodules of Elaeagnus umbellata Confers Resistance on Transgenic Arabidopsis Plants against the Fungal Pathogen Botrytis cinerea ) |
| ;;;;; ;;;;;
|
|
|
 |
|
| |
| 초 록 |
|
|
| Plant chitinases have been known as pathogenesis-related (PR) proteins, but recent studies suggest that they play functional roles during normal plant growth and development. We previously isolated two cDNA clones encoding endochitinases, EuNOD-CHT1 and -CHT2, from the root nodules of Elaeagnus umbellata. These genes show differential expression patterns, with the EuNOD-CHT1 gene being active in the root nodules and meristems, while EuNODCHT2 is preferentially expressed in the infected cells of those nodules. To elucidate the functional roles of these two endochitinases, we have now constitutively expressed each gene in a heterologous plant system, Arabidopsis thaliana. Stable inheritance and expression of the transgenes were confirmed by genomic Southern hybridization and RT-PCR. Our transgenic plants did not differ morphologically from the wild types. However, constitutive expression of EuNOD-CHT1 and -CHT2 in Arabidopsis resulted in increased resistance against a fungal pathogen, Botrytis cinerea, but not against a bacterial agent, Pseudomonas syringae pv. Tomato DC3000. Expression levels were enhanced by both wounding and jasmonic acid treatments (for EuNOD-CHT1), or by jasmonic acid only (for EuNOD-CHT2). These data suggest that EuNOD-CHTl and -CHT2 primarily play defensive roles during root nodule development in E. umbellata. |
| |
| 키워드 |
|
| |
|
|
|
|
Journal of Plant Biology / v.48, no.1, 2005년, pp.39-46
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200532217900807)
언어 : 영어 |
|
| 논문 제공 : KISTI 한국과학기술정보연구원 |
|
|
|
|
|
|
