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한국식물학회 / v.48, no.1, 2005년, pp.85-95 
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( Low Expression Profiles of Heat Stress-Related Genes in Capsicum annuum ) |
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| 초 록 |
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| A cDNA library was constructed for hot pepper plants that had been heat-shock-treated. We used a modified differential screening method, double negative screening, to isolate 500 cDNA clones that represented genes with low expression levels under conditions of high-temperature stress. Of those 500 clones, 200 were randomly selected for single-read sequencing from the 5' ends. After annotation with Blastx, the sequence was applied to InterProScan to scan for functional motifs of proteins. Among the cDNA clones analyzed, about $41%$ of the ESTs could not be functionally classified. However, of those that could be, the largest portion of the ESTs $(15%)$ were assigned to the category of cell rescue and defense; genes involved in cell cycle/DNA processing constituted the smallest group, comprising $1%$ of the ESTs. Genes related to energy and protein fates constituted the second $(10%)$ and third $(9%)$ largest groups, respectively. Finally, $3%$ of the ESTs were assigned to transcription, and $2%$ to signal transduction. The high portion of unclassified ESTs probably resulted from the screening method, which was designed for low-expression messages. Likewise, the high number of ESTs for cell rescue and defense suggests that many genes with low levels of expression are associated with the stress response. |
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| 키워드 |
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Journal of Plant Biology / v.48, no.1, 2005년, pp.85-95
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200532217901095)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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