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한국식물학회 / v.48, no.2, 2005년, pp.237-244 
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( A Double Mutant for the CYP85A1 and CYP85A2 Genes of Arabidopsis Exhibits a Brassinosteroid Dwarf Phenotype ) |
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| 초 록 |
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| Brassinosteroid (BR)-6-oxidases mediate the bridge reactions that connect the late and early C-6 oxidation pathways by converting 6-deoxoBR to 6-oxoBRs. Two similar genes of Arabidopsis, CYP85A1 (At5g38970) and CYP85A2 (At3g30180), are proposed to encode BR-6-oxidases based on findings that heterologously expressed genes mediate BR-6-oxidation reactions in yeast. However, genetic evidence that both genes are critically involved in the BR-6-oxidation step in Arabidopsis has been limited. Here, we show that a double mutant for the two genes displays dwarfism similar to that of typical BR biosynthesis-deficient mutants, suggesting that they are the major BR-6-oxidases in Arabidopsis. Examination of endogenous BR levels and metabolism monitoring tests using this double mutant revealed a great reduction in the levels of 6-oxoBRs, e.g., TY and CS, due to a lack in the conversion reactions from 6-deoxoCS to CS, and from 6-deoxoTY to TY. Surprisingly, the double mutant accumulated a significant amount of 6-oxocampestanol, suggesting that the upstream C-6 oxidation of campestanol to 6-oxocampestanol is not catalyzed by the two BR-6-oxidases in Arabidopsis, rather, by another enzyme yet to be discovered. |
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| 키워드 |
| brassinosteroid;BR-6-oxidase;C-6 oxidation;cell elongation;CYP85;cytochrome P450; |
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Journal of Plant Biology / v.48, no.2, 2005년, pp.237-244
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200504704008489)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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